CAJ | 학술논문

目的建立鉴定长角血蜱和微小扇头蜱的形态学和分子生物学相结合的方法.方法采集自湖北和河南省布尼亚新病毒疫区家养动物体表寄生的蜱及草丛、灌木中的蜱;先以形态学鉴定,后用PCR方法扩增得到蜱的12S rDNA,测序后进行同源性和系统进化分析.结果形态学方法鉴定采集到两种蜱:长角血蜱(Haemaphysalis longicornis)和微小扇头蜱(Rhipicephalus microplus).蜱的12S rDNA经克隆、测序,用PAUP 4.0软件构建系统发生树,湖北、河南省两种蜱的12S rDNA序列分别与长角血蜱和微小扇头蜱聚类,与形态学鉴定结果一致.结论在传统形态学分类的基础上结合分子生物学鉴定方法能更准确地鉴定蜱的种类.
목적 건립감정장각혈비화미소선두비적형태학화분자생물학상결합적방법.방법 채집자호북화하남성포니아신병독역구가양동물체표기생적비급초총、관목중적비;선이형태학감정,후용PCR방법확증득도비적12S rDNA,측서후진행동원성화계통진화분석.결과 형태학방법감정채집도량충비:장각혈비(Haemaphysalis longicornis)화미소선두비(Rhipicephalus microplus).비적12S rDNA경극륭、측서,용PAUP 4.0연건구건계통발생수,호북、하남성량충비적12S rDNA서렬분별여장각혈비화미소선두비취류,여형태학감정결과일치.결론 재전통형태학분류적기출상결합분자생물학감정방법능경준학지감정비적충류.
Objective To establish a method combined morphology and molecular marker for identifying Haemaphysalis longicomis and Rhipicephalus microplus. Methods Ticks were collected from domestic animals and wild environment in epidemic area of Hubei and Henan provinces where cases of fever with thrombocytopenia syndrome were prevalent. We classified the ticks by morphology characteristics before 12S rDNA of ticks were amplified by PCR and subsequently sequenced. Phylogenetic tree was constructed by PAUP4.0. Results The ticks belonged to Haemaphysalis longicomis and Rhipicephalus microplus through observation and analysed by the morphological characteristics of the ticks. 12S rDNA was cloned and sequenced while data confirmed the morphological identification of the results. Conclusion The method based on morphology that combined with molecular marker seemed a good method for the identificaton of ticks.