中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2010年
6期
735-737
,共3页
洪清琦%褚忠华%徐鋆耀%何传超%叶华%曾丽萍%王捷
洪清琦%褚忠華%徐鋆耀%何傳超%葉華%曾麗萍%王捷
홍청기%저충화%서윤요%하전초%협화%증려평%왕첩
肝细胞癌%乙型肝炎病毒%乙型肝炎病毒X蛋白%肿瘤病理分级
肝細胞癌%乙型肝炎病毒%乙型肝炎病毒X蛋白%腫瘤病理分級
간세포암%을형간염병독%을형간염병독X단백%종류병리분급
Hepatocellular carcinoma%Hepatitis B virus%Hepatitis B virus X protein%Tumor pathological grading
目的 检测乙型肝炎病毒x蛋白(HBx)在肝细胞癌中的表达并探讨其与肝癌病理分级的关系.方法 用免疫组织化学法检测41例肝癌患者肿瘤组织中HBx蛋白的表达,与临床资料进行相关分析,并应用逆转录-聚合酶链反应(RT-PCR)、PCR及Western blot法检测肝癌患者肿瘤组织及血清中HBx的表达.结果 肝癌组织中HBx的总体阳性检出率为27/41(65.85%),HBx+与HBx-者肿瘤组织低分化比例分别为11/27(40.74%)及2/14(14.29%),差异有统计学意义(P<0.01),且肿瘤组织低分化比例随HBx阳性表达程度的增加而增加,HBx强阳性表达者肿瘤组织低分化比例高达4/6(66.67%);RT-PCR对肿瘤组织HBx mRNA的检出率为10/16(62.50%),PCR对血清中HBx拷贝的检出率为1/7(14.29%),Western blot对血清中HBx蛋白的检出率则为6/7(85.71%).结论 肝癌肿瘤组织中HBx的表达与肿瘤的病理分级呈负相关,肿瘤组织低分化比例随HBx阳性表达程度的增加而增加.
目的 檢測乙型肝炎病毒x蛋白(HBx)在肝細胞癌中的錶達併探討其與肝癌病理分級的關繫.方法 用免疫組織化學法檢測41例肝癌患者腫瘤組織中HBx蛋白的錶達,與臨床資料進行相關分析,併應用逆轉錄-聚閤酶鏈反應(RT-PCR)、PCR及Western blot法檢測肝癌患者腫瘤組織及血清中HBx的錶達.結果 肝癌組織中HBx的總體暘性檢齣率為27/41(65.85%),HBx+與HBx-者腫瘤組織低分化比例分彆為11/27(40.74%)及2/14(14.29%),差異有統計學意義(P<0.01),且腫瘤組織低分化比例隨HBx暘性錶達程度的增加而增加,HBx彊暘性錶達者腫瘤組織低分化比例高達4/6(66.67%);RT-PCR對腫瘤組織HBx mRNA的檢齣率為10/16(62.50%),PCR對血清中HBx拷貝的檢齣率為1/7(14.29%),Western blot對血清中HBx蛋白的檢齣率則為6/7(85.71%).結論 肝癌腫瘤組織中HBx的錶達與腫瘤的病理分級呈負相關,腫瘤組織低分化比例隨HBx暘性錶達程度的增加而增加.
목적 검측을형간염병독x단백(HBx)재간세포암중적표체병탐토기여간암병리분급적관계.방법 용면역조직화학법검측41례간암환자종류조직중HBx단백적표체,여림상자료진행상관분석,병응용역전록-취합매련반응(RT-PCR)、PCR급Western blot법검측간암환자종류조직급혈청중HBx적표체.결과 간암조직중HBx적총체양성검출솔위27/41(65.85%),HBx+여HBx-자종류조직저분화비례분별위11/27(40.74%)급2/14(14.29%),차이유통계학의의(P<0.01),차종류조직저분화비례수HBx양성표체정도적증가이증가,HBx강양성표체자종류조직저분화비례고체4/6(66.67%);RT-PCR대종류조직HBx mRNA적검출솔위10/16(62.50%),PCR대혈청중HBx고패적검출솔위1/7(14.29%),Western blot대혈청중HBx단백적검출솔칙위6/7(85.71%).결론 간암종류조직중HBx적표체여종류적병리분급정부상관,종류조직저분화비례수HBx양성표체정도적증가이증가.
Objective We aim to detect the expression of Hepatitis B virus X protein (HBx) in hepatocellular carcinoma (HCC) and investigate its relationship with tumor pathological grading. Methods Immunohistochemical method was utilized to detect the expression of HBx protein in tumor tissues of fortyone HCC patients,and the result was analyzed with the clinical data. Reverse transcription-polymerase chain reaction (RT-PCR) ,PCR,and Western blotting were also applied to detect the expression of HBx in tumor tissues and serum of HCC patients. Results The total ratio of HBx positive expression was 27/41 (65. 85%). The poorly differentiated ratios of HBx positive and negative tumor tissues were 11/27 (40. 74%) and 2/14 (14. 29%) respectively, the difference was statistically significant (P<0. 01). And the poorly differentiated ratio of tumor tissues increased with the expression level of HBx, the positive ratio reached 4/6(66. 67%) when HBx was strongly positively expressed. RT-PCR could detect the mRNA of HBx in tumor tissues and the positive ratio was 10/16(62. 50%). Although PCR could detect the HBx copy in the serum of HCC patients,the positive ratio was only 1/7(14. 29%). And Western blotting could efficientlydetect HBx protein in HCC patients' serum,with the highest positive ratio 6/7(85.71%). Conclusion HBx can be detected in both tumor tissues and serum of HCC patients. The expression of HBx is negatively correlated with pathological grading of HCC tumor tissues and the poorly differentiated ratio of tumor tissues increases with the expression level of HBx.
