CAJ | 학술논문

目的探讨白细胞介素(IL)-32在神经胶质瘤中的表达及调节机制. 方法 RT-PCR、Western blotting检测体外培养3d的神经胶质瘤细胞株CHG-5、U251 IL-32 mRNA和蛋白的表达;应用10 ng/mL IL-1β、IL-4、IL-6、IL-10、IL-17、肿瘤坏死因子(TNF)-α、TNF-β、干扰素(IFN)-γ作用U251细胞24 h后RT-PCR检测细胞IL-32 mRNA表达的变化;RT-PCR检测不同浓度IL-1β、TNF-α、IFN-γ作用U251细胞不同时间后IL-32 mRNA表达的变化. 结果 U251细胞IL-32 mRNA表达水平高于CHG-5细胞,IL-32蛋白表达水平(0.95±0.42)高于CHG-5细胞(0.28±0.13),差异均有统计学意义(P＜0.05); IL-1β、TNF-α、IFN-γ作用U251细胞24 h后IL-32 mRNA表达量增加,差异有统计学意义(P＜0.05),且IL-32 mRNA的表达量对IL-1β、TNF-α、IFN-γ刺激的浓度和时间有依赖性. 结论 IL-32在神经胶质瘤中高表达,IL-1β、TNF-α、IFN-γ对IL-32 mRNA的表达具有调节作用,且存在时间与剂量依赖性.
목적 탐토백세포개소(IL)-32재신경효질류중적표체급조절궤제. 방법 RT-PCR、Western blotting검측체외배양3d적신경효질류세포주CHG-5、U251 IL-32 mRNA화단백적표체;응용10 ng/mL IL-1β、IL-4、IL-6、IL-10、IL-17、종류배사인자(TNF)-α、TNF-β、간우소(IFN)-γ작용U251세포24 h후RT-PCR검측세포IL-32 mRNA표체적변화;RT-PCR검측불동농도IL-1β、TNF-α、IFN-γ작용U251세포불동시간후IL-32 mRNA표체적변화. 결과 U251세포IL-32 mRNA표체수평고우CHG-5세포,IL-32단백표체수평(0.95±0.42)고우CHG-5세포(0.28±0.13),차이균유통계학의의(P＜0.05); IL-1β、TNF-α、IFN-γ작용U251세포24 h후IL-32 mRNA표체량증가,차이유통계학의의(P＜0.05),차IL-32 mRNA적표체량대IL-1β、TNF-α、IFN-γ자격적농도화시간유의뢰성. 결론 IL-32재신경효질류중고표체,IL-1β、TNF-α、IFN-γ대IL-32 mRNA적표체구유조절작용,차존재시간여제량의뢰성.
Objective To discuss the expression of interleukin-32 (IL-32) in glioma and its regulatory mechanism. Methods RT-PCR and Western blotting were used to detect the mRNA and protein expression levels of IL-32 in CHG-5 and U251 cell lines in vitro cultured for 3 d.And then, 10 ng/mL IL-1β, IL-4, IL-6, IL-10, IL-17, tumour necrosis factor alpha (TNF-α), TNF-β and interferon-gamma(IFN-γ) were put into U251 cell lines for 24 h,and RT-PCR was employed to detect the mRNA expression of IL-32; meanwhile,RT-PCR was also employed to detect the mRNA expression of IL-32 after the treatment of IL- 1 β,TNF-α and IFN-γ for different times,respectively. Results The mRNA expression of IL-32 in U251 cells was (6.41±1.12)-fold higher than that in CHG-5 cells (P＜0.05);the protein expression level of IL-32 in U251 cells (0.95±0.42) was significantly higher than that in CHG-5 (0.28±0.13,P＜0.05).IL-1β,TNF-α and IFN-γ markedly enhanced the IL-32 mRNA expression at 24 h after treatment as compared with that before treatment, and dose- and time-dependent manners of IL-32 mRNA expression were noted (P＜0.05). Conclusion High expression of IL-32 is noted in glioma; IL-1β, TNF-α and IFN-γ can regulate the IL-32 mRNA expression with dose- and time-dependent manners.