CAJ | 학술논문

外源5.0 mmol/L H2O2和0.1 mmol/L 水杨酸(salicylic acid, SA)处理均可明显提高陈化24 h的马铃薯切片的交替呼吸途径容量(Valt)及其与总呼吸的比值(Valt/Vt).应用交替氧化酶的单克隆抗体进行Western杂交的结果表明,H2O2和SA处理均可明显提高陈化马铃薯切片中交替氧化酶的表达水平.用氧同位素分辨法研究,结果表明:H2O2处理对陈化马铃薯切片中交替呼吸途径的实际运行没有影响,而SA处理对交替呼吸途径的实际运行具有明显的促进作用.上述结果表明,H2O2和SA对植物组织交替呼吸途径的影响存在差异,二者均可促进交替氧化酶的表达从而诱导交替呼吸途径容量的发生,但H2O2不影响其实际运行,而SA还可同时诱导其实际运行.
외원5.0 mmol/L H2O2화0.1 mmol/L 수양산(salicylic acid, SA)처리균가명현제고진화24 h적마령서절편적교체호흡도경용량(Valt)급기여총호흡적비치(Valt/Vt).응용교체양화매적단극륭항체진행Western잡교적결과표명,H2O2화SA처리균가명현제고진화마령서절편중교체양화매적표체수평.용양동위소분변법연구,결과표명:H2O2처리대진화마령서절편중교체호흡도경적실제운행몰유영향,이SA처리대교체호흡도경적실제운행구유명현적촉진작용.상술결과표명,H2O2화SA대식물조직교체호흡도경적영향존재차이,이자균가촉진교체양화매적표체종이유도교체호흡도경용량적발생,단H2O2불영향기실제운행,이SA환가동시유도기실제운행.
The influences of exogenous H2O2 and salicylic acid (SA) treatments on the alternative respiratory pathway (ARP) in aged potato (Solanum tuberosum L.) tuber slices were compared. The results showed that both H2O2 (5.0 mmol/L) and SA (0.1 mmol/L) treatments had a significant inducing effect on ARP capacity (Valt) and its ratio to total respiration (Valt/Vt) in potato slices aged for 24 h. With a monoclonal antibody against the alternative oxidase (AOX), Western blotting results showed that both H2O2 and SA treatments increased the AOX expression levels in aged potato tuber slices. However, the results of oxygen isotope discrimination experiments showed that H2O2 had no influence on the in vivo ARP activity (ρValt) and its contribution to Vt (expressed as ρValt/Vt) in potato slices aged for 24 h, but SA had a significant influence on the ρValt and ρValt/Vt values of the aged potato tuber slices. These results indicate that there are differences between the effects of H2O2 and SA on ARP in plant tissues. Both of them possess the ability to induce ARP capacity through inducing AOX expression. However, SA can simultaneously stimulate the operation of ARP, but H2O2 can not.