天然产物研究与开发
天然產物研究與開髮
천연산물연구여개발
NATURAL PRODUCT RESEARCH AND DEVELOPMENT
2005年
2期
179-181
,共3页
向海艳%周春山%雷启福%肖建波%陈龙胜
嚮海豔%週春山%雷啟福%肖建波%陳龍勝
향해염%주춘산%뢰계복%초건파%진룡성
高效液相色谱法%花生根%白藜芦醇
高效液相色譜法%花生根%白藜蘆醇
고효액상색보법%화생근%백려호순
HPLC%resveratrol%peanut roots
本文采用反相高效液相色谱法测定了花生根中白藜芦醇的含量.选用ODS-C18色谱柱(200 mm×4.6mm,5μm),以甲醇-水(45:55)为流动相,流速为0.5 mL/min,紫外检测波长为303nm.结果表明,白藜芦醇峰面积与其浓度之间呈现良好的线性关系,线性回归方程为:Y=15879.28X+12350.2,相关系数为0.9996.方法简单,快速,精密度好,回收率为98.8%,相对标准偏差为1.79%.
本文採用反相高效液相色譜法測定瞭花生根中白藜蘆醇的含量.選用ODS-C18色譜柱(200 mm×4.6mm,5μm),以甲醇-水(45:55)為流動相,流速為0.5 mL/min,紫外檢測波長為303nm.結果錶明,白藜蘆醇峰麵積與其濃度之間呈現良好的線性關繫,線性迴歸方程為:Y=15879.28X+12350.2,相關繫數為0.9996.方法簡單,快速,精密度好,迴收率為98.8%,相對標準偏差為1.79%.
본문채용반상고효액상색보법측정료화생근중백려호순적함량.선용ODS-C18색보주(200 mm×4.6mm,5μm),이갑순-수(45:55)위류동상,류속위0.5 mL/min,자외검측파장위303nm.결과표명,백려호순봉면적여기농도지간정현량호적선성관계,선성회귀방정위:Y=15879.28X+12350.2,상관계수위0.9996.방법간단,쾌속,정밀도호,회수솔위98.8%,상대표준편차위1.79%.
A resversed-phase high perrormance liquid chromatography(HPLC)method was developed to determine resveratrol in peanut root. The separation was performed on an ODS column(200 × 4.6 mm,5 μm), with the mobile phase of CH3OH-H2O(45:55,v/v).The flow rate was 0.5 mL/min and the detection wavelength was 303 nm. The result showed that there was a good linear relationship between the area and the concentration of resveratrol. The corresponding regression equation is Y =15879.28X + 12350.2,R= 0.9996.The proposed method is simple and rapid with good precion. The average recovery is 98.8% and RSD is 1.79%.
