CAJ | 학술논문

犬瘟热(CD)发病率近年来在我国呈明显上升趋势,对早期CD诊断方法的发展和完善,有利于指导临床及时治疗,对于控制CD的蔓延有重要意义.根据GenBank中CDV核衣壳蛋白(N)基因序列,设计合成一对特异性寡聚核苷酸引物,提取CDV疫苗株的总RNA进行反转录和PCR,建立RT-PCR检测方法,并应用于临床病例的诊断,同时和CDV抗原快速诊断试纸进行比较.结果显示:RT-PCR有很好的敏感性和特异性,适合对CDV进行早期快速诊断和流行病学调查.
견온열(CD)발병솔근년래재아국정명현상승추세,대조기CD진단방법적발전화완선,유리우지도림상급시치료,대우공제CD적만연유중요의의.근거GenBank중CDV핵의각단백(N)기인서렬,설계합성일대특이성과취핵감산인물,제취CDV역묘주적총RNA진행반전록화PCR,건립RT-PCR검측방법,병응용우림상병례적진단,동시화CDV항원쾌속진단시지진행비교.결과현시:RT-PCR유흔호적민감성화특이성,괄합대CDV진행조기쾌속진단화류행병학조사.
In recent years, the incidence of Canine Distemper (CD)disease increases obviously.Developing and perfecting CD diagnostic methods is in favor of guiding clinic therapy in time and is important to control the propagation of CD.According to the reported gene sequence of N protein of Canine Distemper Virus (CDV) in Gene Bank, a special pair of oligonucleotide primers were designed and using the extracted total RNA of CD vaccine strain as a template to set up RT-PCR testing process.Compared the clinic diagnosis effect of RT-PCR testing process with CDV antigen rapid diagnosis test paper,it showed that RT-PCR testing process has good sensitivity and specificity and was adapt to early rapid diagnosis and epidemiological investigation of CDV.