CAJ | 학술논문

考察了不同pH值的碱性溶液中甲醇分子对诱导肌血球素构象变化的影响. 电喷雾电离质谱(ESI-MS)测量结果表明, 当pH分别为11.0, 11.4, 12.0时, 不同体积分数的甲醇(30%, 50%, 70%及90%)和水混合溶液将诱导肌血球素的构象变化. 当pH=11.0时, 肌血球素在溶液中均以hMb为主要形态存在(即血红素稳定存在于肌红蛋白中);当pH=11.4时, 30%甲醇中的肌血球素仍以hMb为主, 但升高甲醇比例会导致hMb上的辅基脱落生成aMb(蛋白质分子);当pH=12.0时, 溶液中的肌血球素均以aMb为主. 但当应用负离子质谱测量模式时, 检测出的肌血球素负离子均以aMb构象为主. 同时采用圆二色谱(CD)及紫外-可见(UV-Vis)光谱等研究发现, pH值和甲醇含量分别决定蛋白质分子的二级和三级结构的变化.
고찰료불동pH치적감성용액중갑순분자대유도기혈구소구상변화적영향. 전분무전리질보(ESI-MS)측량결과표명, 당pH분별위11.0, 11.4, 12.0시, 불동체적분수적갑순(30%, 50%, 70%급90%)화수혼합용액장유도기혈구소적구상변화. 당pH=11.0시, 기혈구소재용액중균이hMb위주요형태존재(즉혈홍소은정존재우기홍단백중);당pH=11.4시, 30%갑순중적기혈구소잉이hMb위주, 단승고갑순비례회도치hMb상적보기탈락생성aMb(단백질분자);당pH=12.0시, 용액중적기혈구소균이aMb위주. 단당응용부리자질보측량모식시, 검측출적기혈구소부리자균이aMb구상위주. 동시채용원이색보(CD)급자외-가견(UV-Vis)광보등연구발현, pH치화갑순함량분별결정단백질분자적이급화삼급결구적변화.
This paper reports the methanol-induced conformation change of myoglobin in basic solution. According to the results of mass spectrometry, we found that the conformation of myoglobin( Mb) changed in different volume fractions of methanol at pH =11.0, 11.4, 12.0. And both of the methanol concentration and pH value have some effect. Mb exists as hMb at pH =11.0, aMb at pH=12.0, aMb at pH=11.4 except in 30% volume fraction of methanol solution. Using negative ion mass spectrometry mode, Mb all show mainly as aMb. More information of Fe ion's coordination and electron spin state were found via CD spectrometry and UV-Vis Soret absorption.