线粒体新变异与中国北方人群中2型糖尿病的关联性的关联性
선립체신변이여중국북방인군중2형당뇨병적관련성적관련성
Association between novel mitochondrial variation and type 2 diabetes in northern Chinese
  • 万方数据
    中国组织工程研究与临床康复 중국조직공정연구여림상강복
    JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
    2009年 46期 9187-9191 ,共5页

    孙飞%唐雷%史晓红%孙亮%冯洁%于君%王晓霞%罗兰%吴迪%万钢%高政南%相蕾%陈静%齐科研%杨泽

    손비%당뢰%사효홍%손량%풍길%우군%왕효하%라란%오적%만강%고정남%상뢰%진정%제과연%양택

    线粒体DNA%2型糖尿病%多态性 線粒體DNA%2型糖尿病%多態性
    선립체DNA%2형당뇨병%다태성
    目的:探讨线粒体DNA 3537A/G、5351A/G位点突变与中国北方人群2型糖尿病发生之间的关系.方法:全部对象均来自大连市,2型糖尿病患者包括大连地区2型糖尿病家系调查收集的患者61例,大连市中心医院内分泌科收治的患者497例及国家"95"攻关"中老年糖尿病普查"大连地区散发患者56例.另选国家"95"攻关"中老年糖尿病普查"中糖耐量正常的344人作为正常对照.在614例2型糖尿病患者和334名非糖尿病对照群体中检测线粒体DNA 3537A/G、5351A/G位点的突变情况.在测序基础上,从线粒体DNA中排序选择2个有较高可能性与2型糖尿病相关的候选SNP,然后使用限制性片断长度多态性聚合酶链反应技术进行基因分型.结果:线粒体DNA 3537A/G、5351A/G在2型糖尿病患者中的突变频率分别为2.0%和2.6%,在对照人群中则分别为2.1%和4.2%,两者之间的差异无显著性意义(P＞0.05).按照体质量指数和血压进行分层之后,发现5351A/G在肥胖的2型糖尿病患者中突变频率为1.61%,在肥胖的对照中频率为15.38%,两者之间的差异具有显著性意义(P_(Fisher)=0.02,OR=2.76);但是A 3537G在各组中的差异无显著性意义(P＞0.05).结论:线粒体DNA ND2基因5351A/G可能与中国北方人群中2型糖尿病发病风险相关.
    목적:탐토선립체DNA 3537A/G、5351A/G위점돌변여중국북방인군2형당뇨병발생지간적관계.방법:전부대상균래자대련시,2형당뇨병환자포괄대련지구2형당뇨병가계조사수집적환자61례,대련시중심의원내분비과수치적환자497례급국가"95"공관"중노년당뇨병보사"대련지구산발환자56례.령선국가"95"공관"중노년당뇨병보사"중당내량정상적344인작위정상대조.재614례2형당뇨병환자화334명비당뇨병대조군체중검측선립체DNA 3537A/G、5351A/G위점적돌변정황.재측서기출상,종선립체DNA중배서선택2개유교고가능성여2형당뇨병상관적후선SNP,연후사용한제성편단장도다태성취합매련반응기술진행기인분형.결과:선립체DNA 3537A/G、5351A/G재2형당뇨병환자중적돌변빈솔분별위2.0%화2.6%,재대조인군중칙분별위2.1%화4.2%,량자지간적차이무현저성의의(P＞0.05).안조체질량지수화혈압진행분층지후,발현5351A/G재비반적2형당뇨병환자중돌변빈솔위1.61%,재비반적대조중빈솔위15.38%,량자지간적차이구유현저성의의(P_(Fisher)=0.02,OR=2.76);단시A 3537G재각조중적차이무현저성의의(P＞0.05).결론:선립체DNA ND2기인5351A/G가능여중국북방인군중2형당뇨병발병풍험상관.
    OBJECTIVE:To explore the association between the mitochondrial DNA(mtDNA)3537A/G,5351A/G variant and type 2diabetes mellitus(T2DM)in northem Chinese population.METHODS:The subjects including 614 patients with T2DM in Dalian City,61 of them were collected from family survey,497 of them were collected from Department of Endocrine,Dalian Municipal Central Hospital,and the remained 56 were selected from diverging T2DM patients in Dalian City.Additional 344 cases with normal carbohydrate toierance were served as controls.The mtDNA 3537A/G.5351A/G variants in 614 patients with T2DM and 334 healthy control subjects were examined.By sequencing the mtDNA in 24 cases and 26 controls,2 candidate SNPs in mtDNA were determined,and then genotyping was carried out by using PCR restriction fragment length polymorphism(PCR-RFLP)analysis.RESULTS:The frequency of mtDNA A3537G and A5351G mutation was 2.0%and 2.6% in T2DM patients,respectively,which was 2.1%and 4.2% in the control group.No significant difference had been observed between case and control(P＞0.05).After stratifying by body mass index and blood pressure,we found that the frequency of A5351 G in obesity patients with T2DM was 1.61%,and in obesity control was 15.38%,which had significant difference(P_(Fisher)=0.02,OR=2.76),however,A3537G stili showed no significant difference in all groups(P＞0.05).CONCLUSION:5351A/G in mtDNA ND2 gene may be a variance associated with T2DM in northem Chinese.
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