解剖学杂志
解剖學雜誌
해부학잡지
CHINESE JOURNAL OF ANATOMY
2010年
1期
23-25,44
,共4页
鼻咽肿瘤%细胞增殖%潜伏膜蛋白1%信号转导与转录活化因子3%生存素%人鼻咽癌细胞
鼻嚥腫瘤%細胞增殖%潛伏膜蛋白1%信號轉導與轉錄活化因子3%生存素%人鼻嚥癌細胞
비인종류%세포증식%잠복막단백1%신호전도여전록활화인자3%생존소%인비인암세포
nasopharyngeal neoplasm%cell proliferation%latent membrane protein 1%signal transducer and activator of transcription 3%survivin%human nasopharyngeal carcinoma cell
目的:探讨EB病毒编码的潜伏膜蛋白1(LMP1)对人鼻咽癌细胞增殖及信号转导与转录活化因子3(STAT3)信号通路的影响.方法:体外培养人高分化鼻咽癌细胞系CNE1及转染了LMP1基因质粒的CNE1细胞(CNE1-GL细胞),通过绘制细胞生长曲线及平板克隆形成实验比较转染LMP1基因前后细胞增殖能力的变化;RT-PCR及免疫印迹方法检测STAT3及其下游基因survivin表达的变化.结果:转染LMP1基因后,CNE1细胞生长加快、克隆形成率增加;STAT3活化程度增加,survivin表达增强.结论:LMP1可促进CNE1细胞增殖,其机制可能是通过活化STAT3信号通路,进而促进抗凋亡基因survivin表达.
目的:探討EB病毒編碼的潛伏膜蛋白1(LMP1)對人鼻嚥癌細胞增殖及信號轉導與轉錄活化因子3(STAT3)信號通路的影響.方法:體外培養人高分化鼻嚥癌細胞繫CNE1及轉染瞭LMP1基因質粒的CNE1細胞(CNE1-GL細胞),通過繪製細胞生長麯線及平闆剋隆形成實驗比較轉染LMP1基因前後細胞增殖能力的變化;RT-PCR及免疫印跡方法檢測STAT3及其下遊基因survivin錶達的變化.結果:轉染LMP1基因後,CNE1細胞生長加快、剋隆形成率增加;STAT3活化程度增加,survivin錶達增彊.結論:LMP1可促進CNE1細胞增殖,其機製可能是通過活化STAT3信號通路,進而促進抗凋亡基因survivin錶達.
목적:탐토EB병독편마적잠복막단백1(LMP1)대인비인암세포증식급신호전도여전록활화인자3(STAT3)신호통로적영향.방법:체외배양인고분화비인암세포계CNE1급전염료LMP1기인질립적CNE1세포(CNE1-GL세포),통과회제세포생장곡선급평판극륭형성실험비교전염LMP1기인전후세포증식능력적변화;RT-PCR급면역인적방법검측STAT3급기하유기인survivin표체적변화.결과:전염LMP1기인후,CNE1세포생장가쾌、극륭형성솔증가;STAT3활화정도증가,survivin표체증강.결론:LMP1가촉진CNE1세포증식,기궤제가능시통과활화STAT3신호통로,진이촉진항조망기인survivin표체.
Objective: To investigate the role of latent membrane protein 1 (LMP1) in the proliferation and STAT3 signaling pathway of nasopharyngeal carcinoma (NPC) cell line CNE1. Methods: CNE1 cells and CNE1-GL cells (CNE1 cells transfected with an eukaryotic LMP1-expression plasmid) were cultured in vitro. Cell growth curves and clone-formation assay were performed to measure the cell proliferation. The expressions of STAT3 and survivin were measured by RT-PCR and Western blot. Results: Transfection of LMP1 gene promoted the proliferation and activation of STAT3 in CNE1 cells, and up-regulated clony forming and expression of phoshated STAT3 (p-STAT3) and survivin. Conclusion: LMP1 may promote the proliferation of CNE1 cells by activating STAT3 signaling pathway.
