检验医学
檢驗醫學
검험의학
LABORATORY MEDICINE
2009年
12期
874-877
,共4页
陆怡德%杨帆%陆秋涯%施新民%樊绮诗
陸怡德%楊帆%陸鞦涯%施新民%樊綺詩
륙이덕%양범%륙추애%시신민%번기시
氨基末端B型钠尿肽原%参考区间%表面健康人群
氨基末耑B型鈉尿肽原%參攷區間%錶麵健康人群
안기말단B형납뇨태원%삼고구간%표면건강인군
N-terminal pro-B-type natriuretic peptide%Reference interval%Apparently healthy individual
目的 调查表面健康人群血清氨基末端B型钠尿肽原(NT-proBNP)的参考区间.方法 参照美国临床实验室标准化委员会(NCCLS)C28-A2文件对德灵公司Dimension X-PAND生化分析仪及NT-proBNP原装配套试剂(简称X-PAND NT-proBNP检测系统)的性能进行验证和评价.检测261名表面健康人群(男127名、女134名)的NT-proBNP水平,通过Z检验,按照性别分组,以第95百分位数分别建立男、女的参考区间.结果 X-PAND NT-proBNP检测系统性能良好,符合临床要求.NT-proBNP随着年龄的增长呈上升趋势,且女性的水平明显高于男性.检测结果 呈偏态分布,＜75岁男性的参考区间为＜64.4 ng/L,＜75岁女性的参考区间为＜122.4 ng/L.结论 对于存在明显种族差异、生物变异的检测项目,每个实验室应参照NCCLS C28-A2对参考区间进行调查或采取纠正措施.建立合适的NT-proBNP参考区间对心力衰竭的诊断、鉴别诊断及预后分析具有临床应用价值.
目的 調查錶麵健康人群血清氨基末耑B型鈉尿肽原(NT-proBNP)的參攷區間.方法 參照美國臨床實驗室標準化委員會(NCCLS)C28-A2文件對德靈公司Dimension X-PAND生化分析儀及NT-proBNP原裝配套試劑(簡稱X-PAND NT-proBNP檢測繫統)的性能進行驗證和評價.檢測261名錶麵健康人群(男127名、女134名)的NT-proBNP水平,通過Z檢驗,按照性彆分組,以第95百分位數分彆建立男、女的參攷區間.結果 X-PAND NT-proBNP檢測繫統性能良好,符閤臨床要求.NT-proBNP隨著年齡的增長呈上升趨勢,且女性的水平明顯高于男性.檢測結果 呈偏態分佈,＜75歲男性的參攷區間為＜64.4 ng/L,＜75歲女性的參攷區間為＜122.4 ng/L.結論 對于存在明顯種族差異、生物變異的檢測項目,每箇實驗室應參照NCCLS C28-A2對參攷區間進行調查或採取糾正措施.建立閤適的NT-proBNP參攷區間對心力衰竭的診斷、鑒彆診斷及預後分析具有臨床應用價值.
목적 조사표면건강인군혈청안기말단B형납뇨태원(NT-proBNP)적삼고구간.방법 삼조미국림상실험실표준화위원회(NCCLS)C28-A2문건대덕령공사Dimension X-PAND생화분석의급NT-proBNP원장배투시제(간칭X-PAND NT-proBNP검측계통)적성능진행험증화평개.검측261명표면건강인군(남127명、녀134명)적NT-proBNP수평,통과Z검험,안조성별분조,이제95백분위수분별건립남、녀적삼고구간.결과 X-PAND NT-proBNP검측계통성능량호,부합림상요구.NT-proBNP수착년령적증장정상승추세,차녀성적수평명현고우남성.검측결과 정편태분포,＜75세남성적삼고구간위＜64.4 ng/L,＜75세녀성적삼고구간위＜122.4 ng/L.결론 대우존재명현충족차이、생물변이적검측항목,매개실험실응삼조NCCLS C28-A2대삼고구간진행조사혹채취규정조시.건립합괄적NT-proBNP삼고구간대심력쇠갈적진단、감별진단급예후분석구유림상응용개치.
Objective To investigate the distribution of reference intervals of serum N-terminal pro-B-type natriuretic peptide (NT-proBNP). Methods According to the National Committee for Clinical Laboratory Standards(NCCLS)C28-A2 Document, the performance of X-PAND NT-proBNP determination system (including the Dade Behring Dimension X-PAND biochemistry analyzer and NT-proBNP original reagent) was analyzed and evaluated. The serum NT-proBNP levels in 261 apparently healthy individuals (127 males, 134 females) were determined. All individuals were classified according to sex. The NT-proBNP reference intervals of males and females were established by 95~(th) percentile respectively. Results The X-PAND NT-proBNP determination system had good analytical performance. Following the increasing age, the NT-proBNP level also increased, and the level in females was higher than that in males. The results of serum NT-proBNP were presented as skewness distribution. The reference interval of NT-proBNP was less than 64.4 ng/L for males and less than 122.4 ng/L for females under the age of 75. Conclusions Clinical laboratories should investigate or establish the appropriate reference intervals for the tests items with ethnic distinction and biomutation. There is a clinical application value to establish the appropriate reference interval for serum NT-proBNP in the diagnosis, differential diagnosis and prognosis analysis of heart failure.
