中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
CHINESE JOURNAL OF BIOCHEMICAL PHARMACEUTICS
2010年
2期
126-127
,共2页
杨小明%赵冬梅%杜秋江%李语如
楊小明%趙鼕梅%杜鞦江%李語如
양소명%조동매%두추강%리어여
高效液相色谱法%比伐卢定%三氟乙酸%含量测定
高效液相色譜法%比伐盧定%三氟乙痠%含量測定
고효액상색보법%비벌로정%삼불을산%함량측정
HPLC%bivalirudin%trifluoroacetic acid%content determination
目的 建立高效液相色谱法测定比伐卢定中三氟乙酸的含量.方法 色谱柱:Kromasil 100-5 C_(18)柱(4.6mm×200 mm,5μm),流动相:0.07%磷酸溶液(pH 3.0)-甲醇(98:2),流速:1.0 mL/min,柱温:30℃,检测波长:210nm,进样量:20μL.结果 三氟乙酸浓度在198.4～992.0 μg/mL范围内,线性关系良好,r=0.999 9;检测限为40 ng;加样回收率平均为99.9%,RSD为0.41%.结论 此法可用于比伐卢定中三氟乙酸含量的测定,准确度高.
目的 建立高效液相色譜法測定比伐盧定中三氟乙痠的含量.方法 色譜柱:Kromasil 100-5 C_(18)柱(4.6mm×200 mm,5μm),流動相:0.07%燐痠溶液(pH 3.0)-甲醇(98:2),流速:1.0 mL/min,柱溫:30℃,檢測波長:210nm,進樣量:20μL.結果 三氟乙痠濃度在198.4～992.0 μg/mL範圍內,線性關繫良好,r=0.999 9;檢測限為40 ng;加樣迴收率平均為99.9%,RSD為0.41%.結論 此法可用于比伐盧定中三氟乙痠含量的測定,準確度高.
목적 건립고효액상색보법측정비벌로정중삼불을산적함량.방법 색보주:Kromasil 100-5 C_(18)주(4.6mm×200 mm,5μm),류동상:0.07%린산용액(pH 3.0)-갑순(98:2),류속:1.0 mL/min,주온:30℃,검측파장:210nm,진양량:20μL.결과 삼불을산농도재198.4～992.0 μg/mL범위내,선성관계량호,r=0.999 9;검측한위40 ng;가양회수솔평균위99.9%,RSD위0.41%.결론 차법가용우비벌로정중삼불을산함량적측정,준학도고.
Purpose To establish a HPLC method for determination of the content of trifluoroacetic acid in bivalirudin.Methods Kromasil 100-5 C_(18) column(4.6 mm×200 mm,5 μm)and 0.07% phosphoric acid solution(pH 3.0)-methanol(98:2)as the mobile phase at a flow rate of 1.0 mL/min were used with a column temperature of 30 ℃,a detection wavelength of 210 nm and a injection volume of 20 μL.Results The standard curve of trifluomacetic acid was linear in the range of 198.4-992.0 μg/mL,r=0.999 9.The detection limit Was 40 ng.The average recovery rate of method was 99.9%,and RSD was 0.41%.Conclusion The method of content determination was practicable and accurate.It can be used for content determination of trifluoroacetic acid in bivalirudin.
