中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2001年
1期
5-8
,共4页
钟学宽%周令望%高彦辉%迟月明%刘阳%万汇娟%刘红%刘艺%张智毅%曾宪惠
鐘學寬%週令望%高彥輝%遲月明%劉暘%萬彙娟%劉紅%劉藝%張智毅%曾憲惠
종학관%주령망%고언휘%지월명%류양%만회연%류홍%류예%장지의%증헌혜
心肌损伤%柯萨奇病毒%重叠感染%低硒
心肌損傷%柯薩奇病毒%重疊感染%低硒
심기손상%가살기병독%중첩감염%저서
目的 探讨Se缺乏、CVB4、CVB3m重叠感染与心肌损伤的关系。方法 采用低Se及相对低蛋白合成饲料、饮用含Se元素水与不含硒的水的方法，饲养小鼠及其所产的幼鼠，于出生7d，幼鼠先后感染CVB4，CVB3m，2型病毒感染间隔为7d，感染CVB3m病毒后7、14、21d，分别处死小鼠，取心脏做光镜、电镜检测。结果 Se缺乏组小鼠，心肌可见轻度损伤，心肌细胞色素氧化酶活力下降。病毒感染7d后，低Se+病毒组小鼠与补Se+病毒组小鼠比较，低硒病毒组血清CVB3m抗体效价1∶256，补硒病毒组中和抗体效价为1∶512，低硒组感染病毒的毒力增强，心肌中病毒分离滴度低硒组明显高于补硒组，心肌细胞色素氧化酶进一步下降，心肌损伤检出率高达63.6%，心肌变性坏死的数量多，损伤面积大。结论 Se缺乏是克山病心肌损害因素之一，而柯萨奇B组2型病毒重叠感染在克山病心肌损伤过程中的作用也不能忽视。硒具有某种程度的抗病毒作用或抑病毒作用。
目的 探討Se缺乏、CVB4、CVB3m重疊感染與心肌損傷的關繫。方法 採用低Se及相對低蛋白閤成飼料、飲用含Se元素水與不含硒的水的方法，飼養小鼠及其所產的幼鼠，于齣生7d，幼鼠先後感染CVB4，CVB3m，2型病毒感染間隔為7d，感染CVB3m病毒後7、14、21d，分彆處死小鼠，取心髒做光鏡、電鏡檢測。結果 Se缺乏組小鼠，心肌可見輕度損傷，心肌細胞色素氧化酶活力下降。病毒感染7d後，低Se+病毒組小鼠與補Se+病毒組小鼠比較，低硒病毒組血清CVB3m抗體效價1∶256，補硒病毒組中和抗體效價為1∶512，低硒組感染病毒的毒力增彊，心肌中病毒分離滴度低硒組明顯高于補硒組，心肌細胞色素氧化酶進一步下降，心肌損傷檢齣率高達63.6%，心肌變性壞死的數量多，損傷麵積大。結論 Se缺乏是剋山病心肌損害因素之一，而柯薩奇B組2型病毒重疊感染在剋山病心肌損傷過程中的作用也不能忽視。硒具有某種程度的抗病毒作用或抑病毒作用。
목적 탐토Se결핍、CVB4、CVB3m중첩감염여심기손상적관계。방법 채용저Se급상대저단백합성사료、음용함Se원소수여불함서적수적방법，사양소서급기소산적유서，우출생7d，유서선후감염CVB4，CVB3m，2형병독감염간격위7d，감염CVB3m병독후7、14、21d，분별처사소서，취심장주광경、전경검측。결과 Se결핍조소서，심기가견경도손상，심기세포색소양화매활력하강。병독감염7d후，저Se+병독조소서여보Se+병독조소서비교，저서병독조혈청CVB3m항체효개1∶256，보서병독조중화항체효개위1∶512，저서조감염병독적독력증강，심기중병독분리적도저서조명현고우보서조，심기세포색소양화매진일보하강，심기손상검출솔고체63.6%，심기변성배사적수량다，손상면적대。결론 Se결핍시극산병심기손해인소지일，이가살기B조2형병독중첩감염재극산병심기손상과정중적작용야불능홀시。서구유모충정도적항병독작용혹억병독작용。
Objective To investigate relation between Se-deficiency andsuperinfection of both CVB4 and CVB3m in mouse myocardial injury.Methods The mice (Kunming line) were divided into 4 groups on the Se contents and superinfection of Coxsackie virus B (CVB). Group1:Se-deficient,without CVB; Group 2: Se-rich without CVB; Group 3:, Se-deficient with CVB; and Group 4: Se-rich with CVB. The mice were fed with Se-deficient food containing lower protein and Se was applied by adding the Se into the drinking water for Se-rich group. For the CVB superinfection groups, the 7-day-old baby mice were inoculated with CVB4. After 1 week interval, the mice inoculated with CVB4 were superinfected with CVB3m .The mice were killed and their myocardial tissue sections were examined under lightmicroscope and electronic microscope at day 7,day 14 and day 21,respectively after CVB3m superinfection.Results The result showed that the myocardium was lightly damaged and the activity of cytochrome oxidase of myocardium decreased in Group 2 compared with those in Group 1.The result also indicated that myocardial injury rate was as high as 63.6%,and the myocardial injury and necrosis area was larger in Grope 4 than those in Group 2 and Group 3.The serological test showed that the titer of neutralizing antibody against CVB3m was 1:256 and 1:512 in Group 4 and in Group 3,respectively.All the results suggested that the interaction between Se-deficiency and superinfection of CVB4and CVB3m intensified the mouse myocardial damage. Conclusions Se-deficiency was one of the factors for myocardial injury in Keshan Disease and the superinfection of two types of CVBs might play a role in the incidence of Keshan Disease and selenium might play roles of antivirus and virus repression to some extent.
