中华内分泌外科杂志
中華內分泌外科雜誌
중화내분비외과잡지
CHINESE JOURNAL OF ENDOCRINE SURGERY
2009年
1期
19-21
,共3页
重症胰腺炎%动物模型%创面封闭胶%顺行穿刺
重癥胰腺炎%動物模型%創麵封閉膠%順行穿刺
중증이선염%동물모형%창면봉폐효%순행천자
Severe acute pancreatitis%Animal model%Medical sealant glue%Antegrade puncture
目的 探讨制备大鼠重症胰腺炎模型方法的改进.方法 将36只健康SD大鼠随机分为模型组(n=18)和假手术组(n=18),在胰胆管的近端(肝总管)用细针顺行穿刺胰胆管,注入4%牛磺胆酸钠,诱导重症急性胰腺炎动物模型,创面封闭胶封闭穿刺孔;两组均在模型诱导3、6、12 h后检测腹水量及血淀粉酶,观察胰腺组织形态变化,光镜下行胰腺病变程度评分.结果 模型组在模型诱导后腹水量(8.52±1.05)ml、血淀粉酶(5247.17±547.07)u/L、胰腺组织学评分(13.60±3.92)较假手术组腹水量(1.21±0.32)ml、血淀粉酶(1289.5±176.67)u/L、胰腺组织学评分(0.83±0.58)在各时间点均明显升高(P<0.05).结论 此法损伤小,实验器材易于获取,操作简单,不会损伤胰腺和发生胆漏,可重复性好,模型稳定,成功率高,是较理想的重症胰腺炎模型制备方法.
目的 探討製備大鼠重癥胰腺炎模型方法的改進.方法 將36隻健康SD大鼠隨機分為模型組(n=18)和假手術組(n=18),在胰膽管的近耑(肝總管)用細針順行穿刺胰膽管,註入4%牛磺膽痠鈉,誘導重癥急性胰腺炎動物模型,創麵封閉膠封閉穿刺孔;兩組均在模型誘導3、6、12 h後檢測腹水量及血澱粉酶,觀察胰腺組織形態變化,光鏡下行胰腺病變程度評分.結果 模型組在模型誘導後腹水量(8.52±1.05)ml、血澱粉酶(5247.17±547.07)u/L、胰腺組織學評分(13.60±3.92)較假手術組腹水量(1.21±0.32)ml、血澱粉酶(1289.5±176.67)u/L、胰腺組織學評分(0.83±0.58)在各時間點均明顯升高(P<0.05).結論 此法損傷小,實驗器材易于穫取,操作簡單,不會損傷胰腺和髮生膽漏,可重複性好,模型穩定,成功率高,是較理想的重癥胰腺炎模型製備方法.
목적 탐토제비대서중증이선염모형방법적개진.방법 장36지건강SD대서수궤분위모형조(n=18)화가수술조(n=18),재이담관적근단(간총관)용세침순행천자이담관,주입4%우광담산납,유도중증급성이선염동물모형,창면봉폐효봉폐천자공;량조균재모형유도3、6、12 h후검측복수량급혈정분매,관찰이선조직형태변화,광경하행이선병변정도평분.결과 모형조재모형유도후복수량(8.52±1.05)ml、혈정분매(5247.17±547.07)u/L、이선조직학평분(13.60±3.92)교가수술조복수량(1.21±0.32)ml、혈정분매(1289.5±176.67)u/L、이선조직학평분(0.83±0.58)재각시간점균명현승고(P<0.05).결론 차법손상소,실험기재역우획취,조작간단,불회손상이선화발생담루,가중복성호,모형은정,성공솔고,시교이상적중증이선염모형제비방법.
Objective To improve the establishment of severe acute pancreatitis model in rat. Methods 36 Sprague-Dawley rats were randomly divided into experiment or shame operation group(n=18,each). Severe acute pancreatitis was induced by injection of 4% sodium taurocholate through puncturing common bile duct with a fine needle, puncture hole was closed by medical sealant glue. Ascites volume and blood amylase were measured at 3, 6 and 12 hours after injection. Gross and histological changes of pancreas were evaluated by a scoring system. Results Pancreatic changes in the experiment group was hemorrhagic and necrosis.The ascites volume(8.52±1.05)ml,serum amylase activity(5247.17±547.07)u/L, gross and histological scores(13.6±3.92) in experiment group was significantly higher than ascites volume(1.21±0.32)ml,serum amylase activity(1289.5±176.67)u/L, gross and histological scores((0.83±0.58) in shame operation group at every time point(P<0.05). Conclusions Combined injection through common bile duct and medical sealant glue in the experiment can minimize the trauma and simplize the procedure. This method produces a reliable model with high success rate and it is an ideal severe acute pancreatitis animal model.
