中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2010年
4期
358-362
,共5页
吴燕峰%任明亮%黄晓明%王潇娉%梁新军%杨睿%黄霖%唐勇%王鹏%沈慧勇
吳燕峰%任明亮%黃曉明%王瀟娉%樑新軍%楊睿%黃霖%唐勇%王鵬%瀋慧勇
오연봉%임명량%황효명%왕소빙%량신군%양예%황림%당용%왕붕%침혜용
嗅鞘细胞%嗅黏膜%嗅球
嗅鞘細胞%嗅黏膜%嗅毬
후초세포%후점막%후구
Olfactory ensheathing cells%Olfactory mucosa%Olfactory bulb
目的 比较成人嗅球与嗅黏膜来源的嗅神经鞘细胞(OECs)的生物学与免疫学特性,探讨后者取代前者行细胞或组织移植治疗的可行性. 方法 分别分离、培养、纯化两种来源的OECs,应用MTT法测定细胞活力,结合对比骨髓间充质干细胞(BMSCs)的免疫特性,应用免疫细胞化学染色、定量技术、流式细胞术、双向混合淋巴细胞反应等方法来比较分析不同组织来源的人OECs的生物学与免疫学特性.结果 两种OECs开始分化时间相同,细胞形态基本一致:生长曲线与活力曲线均走行一致,曲线大部分重合;免疫细胞化学p75~(NTR)染色两者均呈阳性;流式分析两种OECs均不表达CD34与B7-1(CD80)、B7-2(CD86)、HLA-DR、CD40、CD40L等移植免疫标志:嗅球源性嗅神经鞘细胞组双向混合淋巴细胞反应抑制率(51.18%±6.01%)与嗅黏膜源性嗅神经鞘细胞组(51.00%±5.87%)、BMSCs组(52.79%±3.12%)比较,差异均无统计学意义(P<0.05).结论 成人嗅球与嗅黏膜来源的OECs在生物学与免疫学特性方面基本一致,可考虑使用来源更为便捷的成人嗅黏膜源性OECs代替嗅球源性OECs行细胞或组织移植治疗.
目的 比較成人嗅毬與嗅黏膜來源的嗅神經鞘細胞(OECs)的生物學與免疫學特性,探討後者取代前者行細胞或組織移植治療的可行性. 方法 分彆分離、培養、純化兩種來源的OECs,應用MTT法測定細胞活力,結閤對比骨髓間充質榦細胞(BMSCs)的免疫特性,應用免疫細胞化學染色、定量技術、流式細胞術、雙嚮混閤淋巴細胞反應等方法來比較分析不同組織來源的人OECs的生物學與免疫學特性.結果 兩種OECs開始分化時間相同,細胞形態基本一緻:生長麯線與活力麯線均走行一緻,麯線大部分重閤;免疫細胞化學p75~(NTR)染色兩者均呈暘性;流式分析兩種OECs均不錶達CD34與B7-1(CD80)、B7-2(CD86)、HLA-DR、CD40、CD40L等移植免疫標誌:嗅毬源性嗅神經鞘細胞組雙嚮混閤淋巴細胞反應抑製率(51.18%±6.01%)與嗅黏膜源性嗅神經鞘細胞組(51.00%±5.87%)、BMSCs組(52.79%±3.12%)比較,差異均無統計學意義(P<0.05).結論 成人嗅毬與嗅黏膜來源的OECs在生物學與免疫學特性方麵基本一緻,可攷慮使用來源更為便捷的成人嗅黏膜源性OECs代替嗅毬源性OECs行細胞或組織移植治療.
목적 비교성인후구여후점막래원적후신경초세포(OECs)적생물학여면역학특성,탐토후자취대전자행세포혹조직이식치료적가행성. 방법 분별분리、배양、순화량충래원적OECs,응용MTT법측정세포활력,결합대비골수간충질간세포(BMSCs)적면역특성,응용면역세포화학염색、정량기술、류식세포술、쌍향혼합림파세포반응등방법래비교분석불동조직래원적인OECs적생물학여면역학특성.결과 량충OECs개시분화시간상동,세포형태기본일치:생장곡선여활력곡선균주행일치,곡선대부분중합;면역세포화학p75~(NTR)염색량자균정양성;류식분석량충OECs균불표체CD34여B7-1(CD80)、B7-2(CD86)、HLA-DR、CD40、CD40L등이식면역표지:후구원성후신경초세포조쌍향혼합림파세포반응억제솔(51.18%±6.01%)여후점막원성후신경초세포조(51.00%±5.87%)、BMSCs조(52.79%±3.12%)비교,차이균무통계학의의(P<0.05).결론 성인후구여후점막래원적OECs재생물학여면역학특성방면기본일치,가고필사용래원경위편첩적성인후점막원성OECs대체후구원성OECs행세포혹조직이식치료.
Objective To compare the biological and immunological properties of human olfactory ensheathing cells (OECs) derived froom human adult olfactory bulb and olfactory mucosa, and discuss the feasibility that the latter takes the place of the former in the utilization of cell or tissue transplantation therapy. Methods Human OECs derived from different tissues were isolated, cultured and purified; MTT assay was employed to observe the cell viability. With the characteristics of bone mesenchymal stem cells (BMSCs) as comparison, immunocytochemical staining, quantitative techniques,flow cytometry and two-way mixed lymphocytes reaction method were used to compare and analyze the biological and immunological properties of OECs derived from human adult olfactory bulb and olfactory mucosa. Results The 20ECs derived from human adult olfactory bulb and olfactory mucosa started to differentiate at the same time and had the same cell morphology basically. The growth curve was in accordance with the vitality curve and had many coincident points; positive immunohistochemistry P75~(NTR) staining was found in both 2 kinds of OECs. Flow cytometry showed no expressions of CD34, B7-1 (CDS0), B7-2(CD86), HLA-DR, CD40 and CD40L. No significant differences on the rate of two-way mixed lymphocytes reaction were found among HAOBOECs group, HAOMOECs group and BMSCsgroup (51.18%±6.01%, 51.00%±5.87% and 51.00%±5.87%, respectively, P>0.05) Conclusion Human adult olfactory bulb-derived and olfactory mucosa-derived OECs are basically the same biological and immunological properties. Human adult olfactory mucosa-derived OECs, a more convenient source,can take the place of human adult olfactory bulb-derived OECs in the utilization of cell or tissue transplantation therapy.
