CAJ | 학술논문

目的研究缺氧缺血性脑损伤(HIBD)新生大鼠脑组织低氧诱导因子1α(HIF-1α)mRNA的表达及参麦注射液的干预作用.方法新生7 d龄SD大鼠随机分为假手术组、生理盐水组、参麦组,又各分为缺血缺氧后2、12、24 h,3、7、14 d 6个哑组,每亚组9只.采用反转录(RT)-PCR法检测鼠脑HIF-1α mRNA;流式细胞仪膜联蛋白V/碘化丙啶双染色法检测细胞凋亡.结果 (1)缺氧缺血后2 h,生理盐水组和参麦组右侧海马神经元凋亡率即均高于假手术组(均P<0.05),于24 h达到峰值后开始下降[(16.80±1.44)%、(11.95±1.13)%],14 d各组差异均无统计学意义(均P>0.05).参麦组海马神经元凋亡率在缺血缺氧后12、24 h,3和7 d均明显低于生理盐水组(均P<0.05).(2)缺血缺氧后2 h生理盐水组、参麦组新生大鼠右侧大脑中HIF-1α mRNA的表达即均高于假手术组(均P<0.05),于24 h达峰值后开始下降[(她32±4.03)%、(35.63±3.73)%],14 d各组表达差异均无统计学意义(均P>0.05);参麦组大脑中HIF-1α mBNA的表达在缺血缺氧后12、24 h,3和7 d均明显高于生理盐水组(均P<0.05).结论新生大鼠HIBD时HIF-1α mBNA表达增强,参麦注射液可提高新生大鼠HIBD后的HIF-1α mRNA的表达,减少新生大鼠缺氧缺血后神经元凋亡的发生.
목적 연구결양결혈성뇌손상(HIBD)신생대서뇌조직저양유도인자1α(HIF-1α)mRNA적표체급삼맥주사액적간예작용.방법 신생7 d령SD대서수궤분위가수술조、생리염수조、삼맥조,우각분위결혈결양후2、12、24 h,3、7、14 d 6개아조,매아조9지.채용반전록(RT)-PCR법검측서뇌HIF-1α mRNA;류식세포의막련단백V/전화병정쌍염색법검측세포조망.결과 (1)결양결혈후2 h,생리염수조화삼맥조우측해마신경원조망솔즉균고우가수술조(균P<0.05),우24 h체도봉치후개시하강[(16.80±1.44)%、(11.95±1.13)%],14 d각조차이균무통계학의의(균P>0.05).삼맥조해마신경원조망솔재결혈결양후12、24 h,3화7 d균명현저우생리염수조(균P<0.05).(2)결혈결양후2 h생리염수조、삼맥조신생대서우측대뇌중HIF-1α mRNA적표체즉균고우가수술조(균P<0.05),우24 h체봉치후개시하강[(저32±4.03)%、(35.63±3.73)%],14 d각조표체차이균무통계학의의(균P>0.05);삼맥조대뇌중HIF-1α mBNA적표체재결혈결양후12、24 h,3화7 d균명현고우생리염수조(균P<0.05).결론 신생대서HIBD시HIF-1α mBNA표체증강,삼맥주사액가제고신생대서HIBD후적HIF-1α mRNA적표체,감소신생대서결양결혈후신경원조망적발생.
Objective To investigate the effects of Shenmai injection containing active principles of Ginseng and ophiopogon root on the expression of hypoxia—inducible factor 1-α(HIF-1 α)in brain after hypoxic-ischernic brain damage(HIBD)．Methods 108 neonatal SD rats were randomly divided into 2 equal groups：①Shenmai group(Group SM)，undergoing ligation of the right common carotid artery to establish HIBD models,breathing immediately a mixed gas with 8％oxygen and 92％nitrogen for 2 h to cause HI insuh,and then injected intraperitoneally with Shenmal injection 10 ms／kg once a day for 7 d，and ②normal saline group(Group NS)undergoing ligation ofthe right common carotid artery to establish HIBD medels,breathing immediately a mixed gas with 8％oxygen and 92％nitrogen for 2 h，and then injected intraperitoneally with NS 10 ms／ks once a day for 7 d Another 54 neonatal rats underwent sham operation but did not undergo hypoxia as control group(Group C)，2，12，and 24 h，and 3，7，and 14 d after HI insult 9 rats from each group were killed with their right hippecampal tissues taken out Flow cytometry was used to examine the apoptotie rate of the hippocampal neurons．RT-PCR was used to detect the mRNA expression of HIF-1α. Results(1)The apoptesis rate of the-right hippoeampaI tissues began increase 2 h after HI insuh．peaked 24 h after HI，then gradually decreased，and almost returned to the original levels 14 d after HI．There was no significant differences in apoptosis rates 14 d after HI among the 3 groups(all P>0．05)．The neuron apoptosis rates 12 h，24 h，3 d，and 7 d after Hl of Group SM were all significantly Iower than those ofGroup NS(e．g 24 h：(11．95±1．13)％ vs(16．80±1．44)％，all P<0．05)．(2)The HIF-1α mRNA expression level in right brain began to increase 2 h after HI，peaked 24 h after HI，then gradually decreases，and returned to the original level 14 d after HI in both Group SM and Group NS；the HIF-1α mRNA expression in right brain 12 h，24 h，3 d，and 7 d after HI of Group SM were all significantly higher than those of Group NS ( e.g 24 h: ( 44. 32±4.03 ) % vs ( 35.63±3.73 ) %, all P < 0.05 ).Conclusion The HIF-1α mRNA expression in brain tissue is up-regulated after HI insult. Shenmai injection helps increase the mRNA expression of HIF-1α in brain and reduces the apoptosis of hippocampus neurons after HI insult.