中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2012年
10期
3-5
,共3页
异甘草酸镁%肝部分切除%肝再生%肝损伤
異甘草痠鎂%肝部分切除%肝再生%肝損傷
이감초산미%간부분절제%간재생%간손상
Magnesium isoglycyrrhizina%Partial hepatectomy%Liver regeneration%Hepatic injury
目的 研究异甘草酸镁(MgIG)对70％肝部分切除小鼠的保护作用.方法 建立小鼠70％肝部分切除模型后,小鼠被分为生理水10 ml/(kg·d)对照组、MgIG低剂量30 mg/(kg·d)组、MgIG中剂量50 mg/(kg·d)组、MgIG高剂量70 mg/(kg·d)组,每组25只.测定各组术后1、2、3、5、7d的血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)活性,以评估肝功能;并测小鼠肝脏质量及体质量,以计算肝脏体质量比来评估肝再生.另以HE染色,采用光镜观察各组小鼠肝脏组织变化.结果 与生理盐水对照组比,MgIG可降低术后第1、2、3天的血清转氨酶水平(P＜0.05),并提高术后第2、3天的肝脏体质量比(P＜0.05).结论 在小鼠70％肝脏部分切除后,MgIG能维护肝细胞完整,并促进肝再生.
目的 研究異甘草痠鎂(MgIG)對70％肝部分切除小鼠的保護作用.方法 建立小鼠70％肝部分切除模型後,小鼠被分為生理水10 ml/(kg·d)對照組、MgIG低劑量30 mg/(kg·d)組、MgIG中劑量50 mg/(kg·d)組、MgIG高劑量70 mg/(kg·d)組,每組25隻.測定各組術後1、2、3、5、7d的血清中丙氨痠氨基轉移酶(ALT)、天門鼕氨痠氨基轉移酶(AST)活性,以評估肝功能;併測小鼠肝髒質量及體質量,以計算肝髒體質量比來評估肝再生.另以HE染色,採用光鏡觀察各組小鼠肝髒組織變化.結果 與生理鹽水對照組比,MgIG可降低術後第1、2、3天的血清轉氨酶水平(P＜0.05),併提高術後第2、3天的肝髒體質量比(P＜0.05).結論 在小鼠70％肝髒部分切除後,MgIG能維護肝細胞完整,併促進肝再生.
목적 연구이감초산미(MgIG)대70％간부분절제소서적보호작용.방법 건립소서70％간부분절제모형후,소서피분위생리수10 ml/(kg·d)대조조、MgIG저제량30 mg/(kg·d)조、MgIG중제량50 mg/(kg·d)조、MgIG고제량70 mg/(kg·d)조,매조25지.측정각조술후1、2、3、5、7d적혈청중병안산안기전이매(ALT)、천문동안산안기전이매(AST)활성,이평고간공능;병측소서간장질량급체질량,이계산간장체질량비래평고간재생.령이HE염색,채용광경관찰각조소서간장조직변화.결과 여생리염수대조조비,MgIG가강저술후제1、2、3천적혈청전안매수평(P＜0.05),병제고술후제2、3천적간장체질량비(P＜0.05).결론 재소서70％간장부분절제후,MgIG능유호간세포완정,병촉진간재생.
Objective To investigate the protective effect of magnesium isoglycyrrhizina(MgIG)on hepatic injury induced by 70％ partial hepatectomy in mice.Methods Mter suffered from 70％ partial hepatectomy,the mice were divided into control group[treated with saline 10 ml/(kg · d) ],MgIG low dose group [30 mg/(kg · d)],MgIG middle dose group [50 mg/(kg · d)] and MgIG high dose group [ 70 mg/( kg · d) ].There were 25 mice in each group.On 1,2,3,5 and 7 days after 70％ partial hepatectomy,serum activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in each group were measured to assess liver function.Meanwhile,liver weight and body weight were measured and the ratio of liver weight to 100 g body weight was caculated to assess the hepatic regenerative activity.Histological changes in livers of each group were observed by hematoxylin and eosin (HE) staining.Results Compared with saline-treated control group,magnesium isoglycyrrhizina significantly decreased the serum ALT and AST activities on 1st,2nd and 3rd day after hepatectomy( P ＜0.05 ),significantly increased liver weight/100 g body weight ratio on 2nd and 3rd day after hepatectomy ( P ＜ 0.05).Conclusions Magnesium isoglyeyrrhizina can keep hepatocytes intact and enhance hepatic regenerative activity in mice after 70％ partial hepatectomy.
