CAJ | 학술논문

目的：建立在唾液标本中检出EB病毒IgA抗体的方法，以快速、特异诊断鼻咽癌。方法：人工合成EBV-DNA酶及EA-D多肽片段，分别作为捕捉抗原，采用ELISA法检测鼻咽癌患者和健康人血清及唾液中IgA/EBV-DNA酶和EA-D抗体。结果：通过检出O.D值，经统计学处理，选出鼻咽癌诊断阈值为：血清O.D值≥0.3,唾液O.D值≥0.45（DNA酶与EA-D两者阈值一致）。鼻咽癌与健康者两组间DNA酶与EA-D的IgA滴度均为P<0.0001,有非常显著性差异，与病理确诊相比，阳性符合率为72.6％～77.3％。结论：证实EBV合成肽链作为捕捉抗原，唾液为标本，用ELISA法是可行的，方法简便，重复性好，价廉，但诊断符合率需进一步提高。
목적：건립재타액표본중검출EB병독IgA항체적방법，이쾌속、특이진단비인암。방법：인공합성EBV-DNA매급EA-D다태편단，분별작위포착항원，채용ELISA법검측비인암환자화건강인혈청급타액중IgA/EBV-DNA매화EA-D항체。결과：통과검출O.D치，경통계학처리，선출비인암진단역치위：혈청O.D치≥0.3,타액O.D치≥0.45（DNA매여EA-D량자역치일치）。비인암여건강자량조간DNA매여EA-D적IgA적도균위P<0.0001,유비상현저성차이，여병리학진상비，양성부합솔위72.6％～77.3％。결론：증실EBV합성태련작위포착항원，타액위표본，용ELISA법시가행적，방법간편，중복성호，개렴，단진단부합솔수진일보제고。
Objective：This study was designed to establish a salivary EBV-EA IgA and DNase IgA test technique, and seek a fast and specific diagnostic technique for nasopharyngeal carcinoma (NPC). Methods：Polypeptides of EBV-DNase(ED) and EA-D was synthesized as catching antigens. With ELISA technique, IgA/ED and IgA/EA-D were evaluated respectively in saliva and serum from NPC patients and healthy volunteers. Results：After statistic analysis of the optical density(OD) values of samples, the diagnostic criteria of NPC in the examination of either IgA/ED or IgA/EA-D was defined as following：OD≥ 0.3 for serum and OD≥ 0.45 for saliva. Significantly statistical difference existed between the values of either IgA/ED or IgA/EA-D titer in patients with NPC and the values in healthy volunteers,P<0.0001. The coincidence rates between the diagnosis of above IgA/ED or IgA/EA-D titer testes and corresponding histological diagnosis were 72.6％－ 77.3％ . Conclusion： The Elisa test to detect salivary IgA/ED and IgA/EA-D with synthesized polypeptides is a simple, repeatable, and cheap technique with stability and sensitivity. However its coincidence rate with histology should be improved.?