癌症
癌癥
암증
CHINESE JOURNAL OF CANCER
2001年
3期
235-238
,共4页
曾益新%李济忠%简少文%李端%李满枝%宋立兵%张玲%汪慧民
曾益新%李濟忠%簡少文%李耑%李滿枝%宋立兵%張玲%汪慧民
증익신%리제충%간소문%리단%리만지%송립병%장령%왕혜민
EBV-DNA酶%EA-D%肽链%鼻咽癌%唾液%诊断
EBV-DNA酶%EA-D%肽鏈%鼻嚥癌%唾液%診斷
EBV-DNA매%EA-D%태련%비인암%타액%진단
目的:建立在唾液标本中检出EB病毒IgA抗体的方法,以快速、特异诊断鼻咽癌。方法:人工合成EBV-DNA酶及EA-D多肽片段,分别作为捕捉抗原,采用ELISA法检测鼻咽癌患者和健康人血清及唾液中IgA/EBV-DNA酶和EA-D抗体。结果:通过检出O.D值,经统计学处理,选出鼻咽癌诊断阈值为:血清O.D值≥0.3,唾液O.D值≥0.45(DNA酶与EA-D两者阈值一致)。鼻咽癌与健康者两组间DNA酶与EA-D的IgA滴度均为P<0.0001,有非常显著性差异,与病理确诊相比,阳性符合率为72.6%~77.3%。结论:证实EBV合成肽链作为捕捉抗原,唾液为标本,用ELISA法是可行的,方法简便,重复性好,价廉,但诊断符合率需进一步提高。
目的:建立在唾液標本中檢齣EB病毒IgA抗體的方法,以快速、特異診斷鼻嚥癌。方法:人工閤成EBV-DNA酶及EA-D多肽片段,分彆作為捕捉抗原,採用ELISA法檢測鼻嚥癌患者和健康人血清及唾液中IgA/EBV-DNA酶和EA-D抗體。結果:通過檢齣O.D值,經統計學處理,選齣鼻嚥癌診斷閾值為:血清O.D值≥0.3,唾液O.D值≥0.45(DNA酶與EA-D兩者閾值一緻)。鼻嚥癌與健康者兩組間DNA酶與EA-D的IgA滴度均為P<0.0001,有非常顯著性差異,與病理確診相比,暘性符閤率為72.6%~77.3%。結論:證實EBV閤成肽鏈作為捕捉抗原,唾液為標本,用ELISA法是可行的,方法簡便,重複性好,價廉,但診斷符閤率需進一步提高。
목적:건립재타액표본중검출EB병독IgA항체적방법,이쾌속、특이진단비인암。방법:인공합성EBV-DNA매급EA-D다태편단,분별작위포착항원,채용ELISA법검측비인암환자화건강인혈청급타액중IgA/EBV-DNA매화EA-D항체。결과:통과검출O.D치,경통계학처리,선출비인암진단역치위:혈청O.D치≥0.3,타액O.D치≥0.45(DNA매여EA-D량자역치일치)。비인암여건강자량조간DNA매여EA-D적IgA적도균위P<0.0001,유비상현저성차이,여병리학진상비,양성부합솔위72.6%~77.3%。결론:증실EBV합성태련작위포착항원,타액위표본,용ELISA법시가행적,방법간편,중복성호,개렴,단진단부합솔수진일보제고。
Objective:This study was designed to establish a salivary EBV-EA IgA and DNase IgA test technique, and seek a fast and specific diagnostic technique for nasopharyngeal carcinoma (NPC). Methods:Polypeptides of EBV-DNase(ED) and EA-D was synthesized as catching antigens. With ELISA technique, IgA/ED and IgA/EA-D were evaluated respectively in saliva and serum from NPC patients and healthy volunteers. Results:After statistic analysis of the optical density(OD) values of samples, the diagnostic criteria of NPC in the examination of either IgA/ED or IgA/EA-D was defined as following:OD≥ 0.3 for serum and OD≥ 0.45 for saliva. Significantly statistical difference existed between the values of either IgA/ED or IgA/EA-D titer in patients with NPC and the values in healthy volunteers,P<0.0001. The coincidence rates between the diagnosis of above IgA/ED or IgA/EA-D titer testes and corresponding histological diagnosis were 72.6% - 77.3% . Conclusion: The Elisa test to detect salivary IgA/ED and IgA/EA-D with synthesized polypeptides is a simple, repeatable, and cheap technique with stability and sensitivity. However its coincidence rate with histology should be improved.?