CAJ | 학술논문

目的检测龈沟液(gingival cervical fluid,GCF)中核因子活化因子受体配体(receptor activator of nuclear factor kappaB ligand,RANKL)和骨保护素(osteoprotegerin,OPG)水平,探讨GCF中RANKL、OPG及RANKL/OPG与慢性牙周炎的关系.方法采用滤纸条法收集牙周健康者(health,H)和慢性牙周炎患者(chronic periodontitis,CP)GCF样本,用ELISA法检测GCF中RANKL和OPG浓度,同时对检测牙的根尖片进行灰度分析.结果CP组GCF中RANKL浓度(118.93±41.91)pg/μL明显高于H组(39.15±14.83)pg/μL(P＜0.01);CP组GCF中OPG浓度(70.73±23.47)pg/μL明显低于H组(261.28±99.13)pg/μL(P＜0.01);CP组GCF中RANKL/OPG的比值(1.95±1.07)高于H组(0.19±0.13)(P＜0.01).CP组GCF中RANKL和OPG浓度及RANKL/OPG比值与菌斑指数(plaque index,PLI)和龈沟出血指数(sulcus bleeding index,SBI)无明显相关;OPG浓度与牙周探诊深度(probing depth,PD)和牙周附着丧失(attachment loss,AL)呈负相关关系;RANKL浓度和RANKL/OPG比值与患牙根尖片灰度呈负相关关系.结论GCF中RAN-KL和OPG水平及RANKL/OPG的比值与牙周组织的炎症状态相关性不明显,但与慢性牙周炎患者牙周组织的骨破坏有明显相关,提示GCF中RANKL、OPG及RANKL/OPG的比值可作为慢性牙周炎牙槽骨组织破坏的一项较敏感和客观的指标.
목적검측간구액(gingival cervical fluid,GCF)중핵인자활화인자수체배체(receptor activator of nuclear factor kappaB ligand,RANKL)화골보호소(osteoprotegerin,OPG)수평,탐토GCF중RANKL、OPG급RANKL/OPG여만성아주염적관계.방법채용려지조법수집아주건강자(health,H)화만성아주염환자(chronic periodontitis,CP)GCF양본,용ELISA법검측GCF중RANKL화OPG농도,동시대검측아적근첨편진행회도분석.결과CP조GCF중RANKL농도(118.93±41.91)pg/μL명현고우H조(39.15±14.83)pg/μL(P＜0.01);CP조GCF중OPG농도(70.73±23.47)pg/μL명현저우H조(261.28±99.13)pg/μL(P＜0.01);CP조GCF중RANKL/OPG적비치(1.95±1.07)고우H조(0.19±0.13)(P＜0.01).CP조GCF중RANKL화OPG농도급RANKL/OPG비치여균반지수(plaque index,PLI)화간구출혈지수(sulcus bleeding index,SBI)무명현상관;OPG농도여아주탐진심도(probing depth,PD)화아주부착상실(attachment loss,AL)정부상관관계;RANKL농도화RANKL/OPG비치여환아근첨편회도정부상관관계.결론GCF중RAN-KL화OPG수평급RANKL/OPG적비치여아주조직적염증상태상관성불명현,단여만성아주염환자아주조직적골파배유명현상관,제시GCF중RANKL、OPG급RANKL/OPG적비치가작위만성아주염아조골조직파배적일항교민감화객관적지표.