CAJ | 학술논문

[目的]研究VB1对甲基紫精(MV)介导的油菜幼苗氧化胁迫伤害的影响.[方法] 以油菜种子为试验材料,用4种不同浓度的MS、MV、VB1及MV+VB1培养液处理油菜种子,通过测定不同处理下的油菜幼苗鲜重和植物体内SOD、CAT和APX活性以及丙二醛(MDA)和脯氨酸(Pro)的含量,研究VB1对缓解油菜幼苗氧化胁迫作用的影响.[结果] MV可抑制油菜植株的生长,诱导植株体内丙二醛(MDA)和脯氨酸(Pro)含量的增高以及CAT、SOD和APX酶活性的增加;VB1在一定程度上缓解MV介导的油菜幼苗氧化胁迫的伤害,其缓解程度大小与VB1的浓度有关,当VB1的浓度为2.0 mmol/L时,缓解作用最大.[结论] VB1对MV引起的油菜幼苗氧化胁迫有一定的调节作用,其作用大小与MV和VB1的浓度有关.
[목적]연구VB1대갑기자정(MV)개도적유채유묘양화협박상해적영향.[방법] 이유채충자위시험재료,용4충불동농도적MS、MV、VB1급MV+VB1배양액처리유채충자,통과측정불동처리하적유채유묘선중화식물체내SOD、CAT화APX활성이급병이철(MDA)화포안산(Pro)적함량,연구VB1대완해유채유묘양화협박작용적영향.[결과] MV가억제유채식주적생장,유도식주체내병이철(MDA)화포안산(Pro)함량적증고이급CAT、SOD화APX매활성적증가;VB1재일정정도상완해MV개도적유채유묘양화협박적상해,기완해정도대소여VB1적농도유관,당VB1적농도위2.0 mmol/L시,완해작용최대.[결론] VB1대MV인기적유채유묘양화협박유일정적조절작용,기작용대소여MV화VB1적농도유관.
[Objective]The purpose was to study the effect of VB1 on methylviologen (MV)-mediated oxidative stress injury of seedlings of Brassica campestris L. [Method] With the seeds of B. Campestris as the tested materials, they were treated by 4 kinds of medium of MS, MV, VB1 and MV+VB1 with different concn.. Fresh weight of seedlings and SOD, CAT and APX activity, as well as content of malondialdehyde (MDA) and proline (Pro) in B. Campestris plants dealt with different treatments were measured to study the effect of VB1 on alleviating the oxidative stress in seedlings of B. Campestris. [Result] MV could inhibit the growth of B. Campestris plants, induce the increase of contents of MDA and Pro as well as contents of CAT, SOD and APX enzyme activity in plants; VB1 could alleviate the MV-mediated oxidative stress injury in B. Campestris seedlings to some extent. The remission extent was related to concn. Of VB1 and the alleviating effect was the best when VB1 was 2.0 mmol/L. [Conclusion] VB1 had certain regulation on MV-mediated oxidative stress in seedlings of B. Campestris and the remission extent was related to concn. Of MV and VB1.