CAJ | 학술논문

从马尾松林中筛选出的解淀粉芽孢杆菌Bacillus amyloliquefaciens JK-JS3菌株对松材线虫Bursaphelenchus xylophilus具有较高杀线活性.本试验对JK-JS3菌株分泌的杀线活性物质的基本性质进行了研究.结果表明:该菌株产生的杀线组分是可随水分一同蒸发的非蛋白质类物质;其吸收峰在190nm到200nm之间;常温25℃和冷藏4℃下保存60d仍能保持杀线活性;不同温度和pH条件对其杀线组分的活性有影响,加热会降低其活性,碱性条件下具有较高的杀线活性;石油醚、氯仿、乙酸乙酯等有机溶剂不能将其活性物质从粗提液中提取.
종마미송림중사선출적해정분아포간균Bacillus amyloliquefaciens JK-JS3균주대송재선충Bursaphelenchus xylophilus구유교고살선활성.본시험대JK-JS3균주분비적살선활성물질적기본성질진행료연구.결과표명:해균주산생적살선조분시가수수분일동증발적비단백질류물질;기흡수봉재190nm도200nm지간;상온25℃화랭장4℃하보존60d잉능보지살선활성;불동온도화pH조건대기살선조분적활성유영향,가열회강저기활성,감성조건하구유교고적살선활성;석유미、록방、을산을지등유궤용제불능장기활성물질종조제액중제취.
Bacillus amyloliquefaciens JK-JS3, isolated from the leaf of the Pinus massoniana in Mount Zijin, has high nematicidal activity to Bursaphelenchus xylophilus. In order to isolate and purify the ne-maticidal components, the elementary characteristics of the nematicidal crude toxin have been studied in our experiment. The results indicated that the nematicidal toxin is not a protein which can be evaporated with the water and its maximum optical absorption is from 190 to 220 nm. The nematicidal activity of the toxin can sustain for a long period at 25 ℃ and 4 ℃. High temperature and pH condition can affect its nematicidal activity. It has strong nematicidal activity at the alkalinity condition. The nematicidal components can not be dissolved in the organic solvents such as petroleum ether, chloroform and ethyl acetate.