CAJ | 학술논문

[目的]研究鹊肾树(Streblus asper Lour.)叶石油醚萃取物及其组分的体外抗肿瘤活性.[方法]采用MTT法检测鹊肾树叶石油醚萃取物以及经硅胶柱层析分离各组分的体外抗肿瘤活性.[结果]80 μg/ml鹊肾树叶石油醚萃取物对宫颈癌细胞Hela、人胃癌细胞SGC-7901、口腔上皮癌细胞KB作用48 h的抑制百分率分别达到58.6%、53.5%和75.5%.进一步分离纯化鹊肾树叶的石油醚萃取物,所得的FrⅡ组分(80 μg/ml) 对Hela、SGC-7901和卵巢癌细胞Ca-cell的48 h抑制率分别达到69.7%、64.6%和60.2%,FrⅢ组分对Hela、SGC-7901和Ca-cell的48 h抑制率分别达到73.1%、66.1%和52.8%.[结论]鹊肾树叶石油醚萃取物具有一定的抗肿瘤作用,经进一步分离所得的FrⅡ、FrⅢ组分对体外肿瘤细胞增殖呈明显的抑制作用.
[목적]연구작신수(Streblus asper Lour.)협석유미췌취물급기조분적체외항종류활성.[방법]채용MTT법검측작신수협석유미췌취물이급경규효주층석분리각조분적체외항종류활성.[결과]80 μg/ml작신수협석유미췌취물대궁경암세포Hela、인위암세포SGC-7901、구강상피암세포KB작용48 h적억제백분솔분별체도58.6%、53.5%화75.5%.진일보분리순화작신수협적석유미췌취물,소득적FrⅡ조분(80 μg/ml) 대Hela、SGC-7901화란소암세포Ca-cell적48 h억제솔분별체도69.7%、64.6%화60.2%,FrⅢ조분대Hela、SGC-7901화Ca-cell적48 h억제솔분별체도73.1%、66.1%화52.8%.[결론]작신수협석유미췌취물구유일정적항종류작용,경진일보분리소득적FrⅡ、FrⅢ조분대체외종류세포증식정명현적억제작용.
[Objective] The aim was to investigate anticancer effect in vitro of petroleum ether extract from leaves of Streblus asper Lour. and Its Components.[Method]MTT method was adopted to detect petroleum ether extract and anticancer effect in vitro of each component was separated by silica gel column chromatography.[Result]The inhibitive percentage of 80 μg/ml petroleum ether extract on Hela, SGC-7901 and KB cells were 58.6%, 53.5%and 75.5%after 48 hours respectively.After further separation and purification of petroleum ether extract, the inhibitive percentage of fraction FrⅡ(80 μg/ml) on Hela, SGC-7901 and Ca-cell cells were 69.7%,64.6% and 60.2%after 48 hours respectively.And the inhibitive percentage of fraction FrⅢ(80 μg/ml) on Hela, SGC-7901 and Ca-cell cells were 73.1%,66.1% and 52.8%after 48 hours respectively.[Conclusion] Petroleum ether extract from leaves of Streblus asper Lour.has certain anticancer effect.And inhibitory effect of the fraction FrⅡ and FrⅢ seperated from petroleum ether extract on tumor cell proliferation is also obvious.