CAJ | 학술논문

为了研究人类自然抵抗相关巨噬细胞蛋白NRAMP1基因3'UTR多态性与新疆哈萨克族结核病易感性的关系.研究选取新疆哈萨克族活动性结核病患者213例,新疆哈萨克族正常对照者211人,用聚合酶链反应.限制性片断长度多态性(PCR-RFLP)分析的方法对NRAMP1基因3'UTR多态性进行基因分型,比较不同基因型及等位基因的频率,经统计学分析,探讨NRAMP1基因3'UTR多态性与新疆哈萨克族结核病易感性的关系.结果显示,在新疆哈萨克族活动性结核病患者组中NRAMPl基因3'UTRTGTG+/TGTG+基因型138例(64.8%),TGTG+/del基因型63例(29.6%),del/del基因型12例(5.6%);新疆哈萨克族正常对照组TGTG+/TGTG+基因型则为167例(79.1%).TGTG+/del基因型41例(19.5%),del/del基因型3例(1.4%).新疆哈萨克族结核病惠者组TGTG+/del基因型和del/del基因型频率明显高于新疆哈萨克族正常对照组,差异有统计学意义(χ~2=10.8,P<0.01);在新疆哈萨克族结核病患者组的TGTG+等位基因频率为79.6%,del等位基因频率为20.4%.新疆哈萨克族正常对照组中TGTG+和del等住基因频率分别为88.9%和11.1%,del等位基因在新疆哈萨克族结核病患者组中的分布频率高.差异有统计学意义(χ~2:13.7,P<0.01).研究结果提示TGTG缺失等位基因可能是新疆哈萨克族结核病的易感基因.携带TGTG+/del和del/del基因型的新疆哈萨克族人群可能更易患结核病.
위료연구인류자연저항상관거서세포단백NRAMP1기인3'UTR다태성여신강합살극족결핵병역감성적관계.연구선취신강합살극족활동성결핵병환자213례,신강합살극족정상대조자211인,용취합매련반응.한제성편단장도다태성(PCR-RFLP)분석적방법대NRAMP1기인3'UTR다태성진행기인분형,비교불동기인형급등위기인적빈솔,경통계학분석,탐토NRAMP1기인3'UTR다태성여신강합살극족결핵병역감성적관계.결과현시,재신강합살극족활동성결핵병환자조중NRAMPl기인3'UTRTGTG+/TGTG+기인형138례(64.8%),TGTG+/del기인형63례(29.6%),del/del기인형12례(5.6%);신강합살극족정상대조조TGTG+/TGTG+기인형칙위167례(79.1%).TGTG+/del기인형41례(19.5%),del/del기인형3례(1.4%).신강합살극족결핵병혜자조TGTG+/del기인형화del/del기인형빈솔명현고우신강합살극족정상대조조,차이유통계학의의(χ~2=10.8,P<0.01);재신강합살극족결핵병환자조적TGTG+등위기인빈솔위79.6%,del등위기인빈솔위20.4%.신강합살극족정상대조조중TGTG+화del등주기인빈솔분별위88.9%화11.1%,del등위기인재신강합살극족결핵병환자조중적분포빈솔고.차이유통계학의의(χ~2:13.7,P<0.01).연구결과제시TGTG결실등위기인가능시신강합살극족결핵병적역감기인.휴대TGTG+/del화del/del기인형적신강합살극족인군가능경역환결핵병.
To determine whether 3'UTR polymorphisms of NRAMP1 gene are asocciated with tuberculosis in Hazakhs of Xinjiang, 3'UTR polymorphisms of NRAMPl gene was typed by PCR-RLFP(polymerase chain reaction-restriction fragment length polymorphisms) among 213 patients with active tuberculosis in Hazakhs of Xinjiang and 211 healthy individuals in Hazakhs of Xinjiang. The relationship of 3' UTR polymorphisms and susceptibility to tuberculosis was studied. Results showed that the genotype TGTG+/ TGTG+, TGTG + /del and del /del were observed in 138, 63 and 12 cases among tuberculosis group while in 167, 41 and 3 cases among control group. The frequency of genotype TGTG + /del and del/del were found more often among patients than that in controls (χ~2=10.8, P<0.01). The TGTG+ and del allele frequencies were 0.796, 0.204 respectively in tuberculosis group and 0.889, 0.111 in normal group. Theallele frequencies of del were statisticly significant in tuberculosis group compared with the control group(χ~2=13.7, P<0.01) in Hazakhs of Xinjiang. The TGTGdel allele may be a risk factor for the tuberculosis ofHazakhs in Xinjiang and the Hazakhs carrying TGTG + / del and del / del genotype are apt to suffertuberculosis.