中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2012年
7期
466-469
,共4页
华方方%夏永华%吴大鹏%陈瑞香%王玉红%潘莹%杨君%梁武风
華方方%夏永華%吳大鵬%陳瑞香%王玉紅%潘瑩%楊君%樑武風
화방방%하영화%오대붕%진서향%왕옥홍%반형%양군%량무풍
宫颈肿瘤%组蛋白去乙酰基酶2%细胞增殖%细胞周期%细胞周期蛋白类%细胞系,肿瘤
宮頸腫瘤%組蛋白去乙酰基酶2%細胞增殖%細胞週期%細胞週期蛋白類%細胞繫,腫瘤
궁경종류%조단백거을선기매2%세포증식%세포주기%세포주기단백류%세포계,종류
Uterine cervical neoplasms%Histone deacetylase 2%Cell proliferation%Cell cycle%Cyclins%Cell line,tumor
目的 探讨组蛋白去乙酰化酶2(HDAC2)表达下调对宫颈癌细胞增殖和细胞周期的影响,并分析其可能的分子机制.方法 将HDAC2小干扰RNA(siRNA)和对照siRNA转染宫颈癌HeLa细胞,采用细胞增殖与活性检测试剂盒(CCK-8)分析转染前后细胞增殖的变化,利用流式细胞术检测转染前后细胞周期分布的变化,采用Western blot方法分析与细胞增殖和细胞周期相关蛋白表达的变化.结果 HDAC2 siRNA能明显下调宫颈癌细胞中HDAC2蛋白的表达,其表达下调能明显抑制宫颈癌细胞的增殖.HDAC2 siRNA组中HeLa细胞在G0/G1期的细胞比率为63.3％±2.0％,显著高于未处理组(29.3％±1.7％)和对照siRNA组(29.4％±1.7％),F=354.181,P=0.000.Western blot结果显示,HDAC2表达下调能明显抑制cyclin D1、cyclin E和CDK2蛋白的表达,增加p21蛋白的表达.结论 HDAC2表达下调介导的细胞增殖抑制和周期阻滞可能与cyclin D1、cyclin E和CDK2蛋白表达的下调和p21蛋白表达的升高密切相关.
目的 探討組蛋白去乙酰化酶2(HDAC2)錶達下調對宮頸癌細胞增殖和細胞週期的影響,併分析其可能的分子機製.方法 將HDAC2小榦擾RNA(siRNA)和對照siRNA轉染宮頸癌HeLa細胞,採用細胞增殖與活性檢測試劑盒(CCK-8)分析轉染前後細胞增殖的變化,利用流式細胞術檢測轉染前後細胞週期分佈的變化,採用Western blot方法分析與細胞增殖和細胞週期相關蛋白錶達的變化.結果 HDAC2 siRNA能明顯下調宮頸癌細胞中HDAC2蛋白的錶達,其錶達下調能明顯抑製宮頸癌細胞的增殖.HDAC2 siRNA組中HeLa細胞在G0/G1期的細胞比率為63.3％±2.0％,顯著高于未處理組(29.3％±1.7％)和對照siRNA組(29.4％±1.7％),F=354.181,P=0.000.Western blot結果顯示,HDAC2錶達下調能明顯抑製cyclin D1、cyclin E和CDK2蛋白的錶達,增加p21蛋白的錶達.結論 HDAC2錶達下調介導的細胞增殖抑製和週期阻滯可能與cyclin D1、cyclin E和CDK2蛋白錶達的下調和p21蛋白錶達的升高密切相關.
목적 탐토조단백거을선화매2(HDAC2)표체하조대궁경암세포증식화세포주기적영향,병분석기가능적분자궤제.방법 장HDAC2소간우RNA(siRNA)화대조siRNA전염궁경암HeLa세포,채용세포증식여활성검측시제합(CCK-8)분석전염전후세포증식적변화,이용류식세포술검측전염전후세포주기분포적변화,채용Western blot방법분석여세포증식화세포주기상관단백표체적변화.결과 HDAC2 siRNA능명현하조궁경암세포중HDAC2단백적표체,기표체하조능명현억제궁경암세포적증식.HDAC2 siRNA조중HeLa세포재G0/G1기적세포비솔위63.3％±2.0％,현저고우미처리조(29.3％±1.7％)화대조siRNA조(29.4％±1.7％),F=354.181,P=0.000.Western blot결과현시,HDAC2표체하조능명현억제cyclin D1、cyclin E화CDK2단백적표체,증가p21단백적표체.결론 HDAC2표체하조개도적세포증식억제화주기조체가능여cyclin D1、cyclin E화CDK2단백표체적하조화p21단백표체적승고밀절상관.
Objective To study the effect of down-regulation of histone deacetylase 2(HDAC2) expression on cell proliferation and cell cycle in cervical carcinoma cell lines HeLa,Methods HDAC2 siRNA and control siRNA were transfected to HeLa cells.CCK-8 and flow cytometry were used to analyze the changes of cell proliferation and cell cycle,respectively.Western blot was employed to detect the changes of cell proliferation and cell cycle-related proteins.Results HDAC2 siRNA significantly down-regulated the expression of HDAC2 protein in HeLa cells,resulting in marked inhibition of cell proliferation.In addition,the percentage of cells in G0/G1 phase in HDAC2 siRNA group(63.3％ ± 2.0％) was significantly higher than that in untreated group(29.3 ％ ± 1.7％) or control siRNA group(29.4％ ± 1.7％),F =354.181,P =0.000.Furthermore,Western blot demonstrated that down-regulation of HDAC2 expression decreased the expression of cyclin D1,cyclin E and CDK2 proteins but increased the expression of p21 protein.Conclusions Down-regulation of ttDAC2 expression mediates proliferation inhibition and cell cycle arrest.It is associated with decrease in cyclin D1,cyclin E and CDK2 protein expression and increase in p21 protein expression.
