中国实用眼科杂志
中國實用眼科雜誌
중국실용안과잡지
CHINESE JOURNAL OF PRACTICAL OPHTHALMOLOGY
2009年
9期
1055-1058
,共4页
孟根托娅%闫元奎%赵海霞
孟根託婭%閆元奎%趙海霞
맹근탁아%염원규%조해하
视网膜脱离%碱性成纤维细胞生长因子(bFGF)%细胞凋亡
視網膜脫離%堿性成纖維細胞生長因子(bFGF)%細胞凋亡
시망막탈리%감성성섬유세포생장인자(bFGF)%세포조망
Retinal detachment%Basic fibroblast growth factor%Apoptosis
目的 研究实验性视网膜脱离(RD)后家兔视网膜细胞凋亡情况,探讨RD后视功能损伤机制,观察碱性成纤维细胞生长因子(bFGF)对细胞凋亡的干预作用.方法 从42只青紫兰兔中随机选取40只兔,将每只兔的左、右眼分为实验对照组和治疗组.视网膜下注射玻璃酸钠(爱维)建立RD动物模型后,取右眼在玻璃体腔内注射10IU/20μl bFGF溶液,作为治疗组;左眼注射平衡盐溶液(BSS)20μl作为实验对照组.剩余2只家兔设为正常对照组,不作任何处理,在实验观察结束时取眼球.三组均采用HE染色、原位末端标记法(TUNEL)和透射电子显微镜观察视网膜细胞凋亡情况,并进行细胞计数和统计学检验.结果 RD早期就有细胞凋亡的发生.视网膜各层细胞均可见凋亡细胞,内核层、节细胞层分布最多.凋亡细胞数随脱离时间延长而变化(P<0.01).bFGF组的凋亡细胞数比BSS组少(P<0.01).电镜下可见除正常对照组基本无凋亡细胞外,其余两组均观察到凋亡细胞,.bFGF组中典型凋亡细胞明显少于实验对照组.结论 实验性RD时视网膜组织细胞中存在异常凋亡,可能是RD后视功能损伤机制之一.玻璃体腔内给予bFGF可以抑制视网膜细胞凋亡,为临床治疗视网膜脱离提供参考.
目的 研究實驗性視網膜脫離(RD)後傢兔視網膜細胞凋亡情況,探討RD後視功能損傷機製,觀察堿性成纖維細胞生長因子(bFGF)對細胞凋亡的榦預作用.方法 從42隻青紫蘭兔中隨機選取40隻兔,將每隻兔的左、右眼分為實驗對照組和治療組.視網膜下註射玻璃痠鈉(愛維)建立RD動物模型後,取右眼在玻璃體腔內註射10IU/20μl bFGF溶液,作為治療組;左眼註射平衡鹽溶液(BSS)20μl作為實驗對照組.剩餘2隻傢兔設為正常對照組,不作任何處理,在實驗觀察結束時取眼毬.三組均採用HE染色、原位末耑標記法(TUNEL)和透射電子顯微鏡觀察視網膜細胞凋亡情況,併進行細胞計數和統計學檢驗.結果 RD早期就有細胞凋亡的髮生.視網膜各層細胞均可見凋亡細胞,內覈層、節細胞層分佈最多.凋亡細胞數隨脫離時間延長而變化(P<0.01).bFGF組的凋亡細胞數比BSS組少(P<0.01).電鏡下可見除正常對照組基本無凋亡細胞外,其餘兩組均觀察到凋亡細胞,.bFGF組中典型凋亡細胞明顯少于實驗對照組.結論 實驗性RD時視網膜組織細胞中存在異常凋亡,可能是RD後視功能損傷機製之一.玻璃體腔內給予bFGF可以抑製視網膜細胞凋亡,為臨床治療視網膜脫離提供參攷.
목적 연구실험성시망막탈리(RD)후가토시망막세포조망정황,탐토RD후시공능손상궤제,관찰감성성섬유세포생장인자(bFGF)대세포조망적간예작용.방법 종42지청자란토중수궤선취40지토,장매지토적좌、우안분위실험대조조화치료조.시망막하주사파리산납(애유)건립RD동물모형후,취우안재파리체강내주사10IU/20μl bFGF용액,작위치료조;좌안주사평형염용액(BSS)20μl작위실험대조조.잉여2지가토설위정상대조조,불작임하처리,재실험관찰결속시취안구.삼조균채용HE염색、원위말단표기법(TUNEL)화투사전자현미경관찰시망막세포조망정황,병진행세포계수화통계학검험.결과 RD조기취유세포조망적발생.시망막각층세포균가견조망세포,내핵층、절세포층분포최다.조망세포수수탈리시간연장이변화(P<0.01).bFGF조적조망세포수비BSS조소(P<0.01).전경하가견제정상대조조기본무조망세포외,기여량조균관찰도조망세포,.bFGF조중전형조망세포명현소우실험대조조.결론 실험성RD시시망막조직세포중존재이상조망,가능시RD후시공능손상궤제지일.파리체강내급여bFGF가이억제시망막세포조망,위림상치료시망막탈리제공삼고.
Objective To investigate the interference effect of basic fibroblast growth factor (bFGF) on apoptosis of retinal cells in experimental retinal detachment (RD).At the same time to observe the changes of apoptosis of retinal cells, and explore the pathoganesis of RD.Methods Forty cyanotic blue rabbits were selected randomly from 42 rabbits, and the left and right eyes were in the experimental control group and bFGF group, respectively.After the RD model was set up by subretinal injection with 20μ 1 of sodium byahironate; bFGF (10IU / 20μ 1) was injected into the vitreous body of the right eyes as the bFGF group; 20μ 1 BSS (balanced salt solution) was injected into vitreous body of left eyes as experimental control group.Another 2 rabbits were selected as the normal control group, which underwent none of the injections.At the end of the observation period eyeballs of the two rabbits were extracted.Routine pathological examination, TUNEL (TdT-mediated dUTP nick end labeling) and Transmission electron microscopy were used to detect the apoptosis of the retinal cells.Cell counts and statistical analysis were used to assess the results.Results Typical apoptosis cells were observed in the early time of RD.Apoptosis cells was found in each retinal layers, especially in inner nuclear layers and Ganglion cell layer.The number of apoptosis cells changed as the time of RD was prolonged (P<0.01).It was also found that apoptosis cells in bFGF group were less than that in the experimental control group (P<0.01).Typical apoptotic cells in bFGF group were less than experimental control group.Canclusions In the experiment of RD, abnormal apoptosis was observed in the retinal cells.Apoptosis may be one of the mechanisms of visual function injury after RD. Intravitreous injection of exogenous bFGF may inhibit the apoptosis of retinal cells in experimental RD.
