CAJ | 학술논문

利用30个微卫星分子标记对海南鲤(HN)、长江鲤(CY)、月亮湖鲤(YL)、黑龙江鲤(FY)、呼伦湖鲤(ZL)、贝尔湖鲤(BR) 6个野生鲤鱼群体的观测杂合度(Ho)、期望杂合度(He)、多态信息含量(PIC)和有效等位基因数(Ae)等进行了遗传检测,根据基因频率计算遗传相似系数和Nei氏标准遗传距离,χ2检验估计Hardy-Weinberg平衡,用近交系数(FST)和基因流(Nm)分析群体的遗传分化及其来源.同时,使用PHYLIP3.63软件绘制基于Nei氏标准遗传距离的UPGMA进化树.6个群体共检测到8,136个扩增片段,长度在125bp ～ 414bp,30个基因座扩增出等位基因数从3 ～ 13个不等,共计210个等位基因,平均每个基因座扩增得到7个等位基因.结果显示:(1) 6个野鲤群体的多态性指标均适中,多态信息含量依次0.44、0.52、0.53、0.57、0.63和0.64,有效等位基因数1.04 ～ 4.72个不等,平均有效等位基因数依次为2.19、2.60、2.42、2.43、2.45和2.33,无偏期望杂合度平均值为0.50、0.59、0.56、0.56、0.57和0.54;(2) 遗传相似系数BR与ZL最高(0.8511),BR与HN最低(0.6688),聚类结果与地理分布呈一定相关性.
이용30개미위성분자표기대해남리(HN)、장강리(CY)、월량호리(YL)、흑룡강리(FY)、호륜호리(ZL)、패이호리(BR) 6개야생리어군체적관측잡합도(Ho)、기망잡합도(He)、다태신식함량(PIC)화유효등위기인수(Ae)등진행료유전검측,근거기인빈솔계산유전상사계수화Nei씨표준유전거리,χ2검험고계Hardy-Weinberg평형,용근교계수(FST)화기인류(Nm)분석군체적유전분화급기래원.동시,사용PHYLIP3.63연건회제기우Nei씨표준유전거리적UPGMA진화수.6개군체공검측도8,136개확증편단,장도재125bp ～ 414bp,30개기인좌확증출등위기인수종3 ～ 13개불등,공계210개등위기인,평균매개기인좌확증득도7개등위기인.결과현시:(1) 6개야리군체적다태성지표균괄중,다태신식함량의차0.44、0.52、0.53、0.57、0.63화0.64,유효등위기인수1.04 ～ 4.72개불등,평균유효등위기인수의차위2.19、2.60、2.42、2.43、2.45화2.33,무편기망잡합도평균치위0.50、0.59、0.56、0.56、0.57화0.54;(2) 유전상사계수BR여ZL최고(0.8511),BR여HN최저(0.6688),취류결과여지리분포정일정상관성.
Thirty microsatellite loci were used for analyzing six wild populations of common carp (Cyprinus carpio L.).Observed (Ho) and expected (He) heterozygosity values,polymorphic information content (PIC),and number of effective alleles (Ae) were all detected.Genetic similarity index and genetic distance were computed based on the allele frequency.The Hardy-Weinberg Equilibrium was checked according to the test of χ2.Genetic differentiation and hierarchical partition of genetic diversity were evaluated by FST and Nm.A clustering dendrogram was made based on the results of UPGMA methods using the PHYLIP software package (version 3.63).There were totally 8,136 fragments ranging from 125 bp to 414 bp in length.Three to thirteen alleles were amplified in 30 loci and 210 alleles in all six populations.The average number of alleles in each locus was seven.The result showed that 1) the level of genetic variability was moderate in the six populations.Polymorphic information contents of the six wild common carp populations were 0.44,0.52,0.53,0.57,0.63,and 0.64 respectively.Effective alleles were from 1.04 to 4.72,the average numbers in each population were 2.19,2.60,2.42,2.43,2.45,and 2.33.The average expected heterozygosity values were 0.50,0.59,0.56,0.56,0.57,and 0.54 respectively;2) the highest genetic similarity index that came from the populations of BR and ZL was 0.8511 and the lowest index was 0.6688,and it came from the populations of BR and HN.There was a correlation between the clustering result and the geographical distribution.