CAJ | 학술논문

目的以细菌胞外多糖黏附素(PIA)产生作为毒力指标,鉴定凝固酶阴性葡萄球菌(CNS)的致病性.方法用刚果红琼脂培养法分离CNS,用刚果红-阿里新蓝对PIA进行联合染色,同时,与采自皮肤的CNS样本作阴性对照.结果致病性CNS在刚果红琼脂培养基中菌落呈黑色,阴性对照组的菌落为红色.染色镜下观察,致病性CNS菌体着色较深,菌体表面PIA显深红色;对照组菌体无PIA而为淡红色.结论刚果红琼脂培养法和刚果红-阿里新蓝联合染色,对CNS的分离和PIA的检测有重要意义,能为CNS致病性的确定提供可靠依据.
목적 이세균포외다당점부소(PIA)산생작위독력지표,감정응고매음성포도구균(CNS)적치병성.방법 용강과홍경지배양법분리CNS,용강과홍-아리신람대PIA진행연합염색,동시,여채자피부적CNS양본작음성대조.결과 치병성CNS재강과홍경지배양기중균락정흑색,음성대조조적균락위홍색.염색경하관찰,치병성CNS균체착색교심,균체표면PIA현심홍색;대조조균체무PIA이위담홍색.결론 강과홍경지배양법화강과홍-아리신람연합염색,대CNS적분리화PIA적검측유중요의의,능위CNS치병성적학정제공가고의거.
Objective With polysaccharide intercellular adhesion (PIA) as a potential indicator of virulence,the pathogenicity of coagulase-negative staphylococci (CNS) was determined. Methods CNS strains were isolated with Congo red agar culture. Then Congo red and Alcian blue stains were used to perform combined staining for PIA with the skin CNS sample as negative control. Results A positive result of pathogenic CNS on Congo red agar (CRA) was shown as black colonies and the nega-tive control usually remained pink. After combined staining, pathogenic CNS strains with PIA positive showed as cardinal red and the negative control strain as salmon pink observed under light microscopy. Conclusion Isolation of CNS with Congo red agar culture method and detection of PIA with Congo red-Alcian blue combined staining might offer reliable experimental basis for definiting pathogenicity of CNS.