中国中西医结合杂志
中國中西醫結閤雜誌
중국중서의결합잡지
CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE
2009年
10期
905-909
,共5页
肖青川%兑丹华%兰天罡%李开伦
肖青川%兌丹華%蘭天罡%李開倫
초청천%태단화%란천강%리개륜
急性坏死性胰腺炎%细菌移位%高迁徙率族蛋白-1%一氧化氮%内皮素-1%清胰Ⅱ号颗粒剂
急性壞死性胰腺炎%細菌移位%高遷徙率族蛋白-1%一氧化氮%內皮素-1%清胰Ⅱ號顆粒劑
급성배사성이선염%세균이위%고천사솔족단백-1%일양화담%내피소-1%청이Ⅱ호과립제
acute necrotizing pancreatitis%bacterial translocation%high mobility group box 1%nitric oxide%endothelin-1%Qingyi Ⅱ granule
目的 观察清胰Ⅱ号颗粒剂对急性坏死性胰腺炎(acute necrotizing pancreatitis,ANP)大鼠肠道细菌移位的影响并探讨其机理.方法 将18只SD大鼠随机等分为假手术组、ANP组及治疗组,每组6只;ANP组及治疗组以30 g/L牛磺胆酸钠逆行注入胰胆管制作大鼠ANP模型,造模术后1 h,治疗组以250 g/L清胰Ⅱ号颗粒剂灌胃给药(10 mL/kg),6 h 1次,共3次,假手术组及ANP组用同等剂量生理盐水以相同方式灌胃.造模后24 h,取大鼠肝脏、胰腺、脾脏、肠系膜淋巴结组织及回肠内容物行细菌培养及菌种鉴定,用real-time PCR检测回肠组织中高迁徙率族蛋白-1(high mobility group box 1,Hmgbl)mRNA表达,ELISA测定回肠组织中一氧化氮(nitric oxide,NO)、内皮素-1(endothelin-1,ET-1)浓度,同时观察胰腺、回肠组织病理改变,测定回肠组织湿/干重比值.结果 ANP组胰腺、回肠组织损伤明显,回肠组织Hmgb1 mRNA的表达较假手术组上调(P<0.01),NO、ET-1浓度较假手术组升高(P<0.01),分别为(1.67±0.21)μmol/L、(102.18±9.19)ng/L,细菌移位至肝脏、胰腺、脾脏及肠系膜淋巴结;治疗组回肠组织中Hmgb1 mRNA的表达较ANP组下调(P<0.01),NO、ET-1浓度较ANP组降低(P<0.05),分别为(1.39±0.23)μmol/L、(83.15±5.39)ng/L,回肠病理损害程度减轻,细菌移位减少.结论 Hmgb1、NO及ET-1浓度在ANP模型大鼠肠道细菌移位中可能具有重要作用.清胰Ⅱ号颗粒剂可能通过下调回肠组织中Hmgb1 mRNA的表达,降低NO、ET-1浓度,减轻胰腺、回肠组织的病理改变,从而抑制肠道细菌移位.
目的 觀察清胰Ⅱ號顆粒劑對急性壞死性胰腺炎(acute necrotizing pancreatitis,ANP)大鼠腸道細菌移位的影響併探討其機理.方法 將18隻SD大鼠隨機等分為假手術組、ANP組及治療組,每組6隻;ANP組及治療組以30 g/L牛磺膽痠鈉逆行註入胰膽管製作大鼠ANP模型,造模術後1 h,治療組以250 g/L清胰Ⅱ號顆粒劑灌胃給藥(10 mL/kg),6 h 1次,共3次,假手術組及ANP組用同等劑量生理鹽水以相同方式灌胃.造模後24 h,取大鼠肝髒、胰腺、脾髒、腸繫膜淋巴結組織及迴腸內容物行細菌培養及菌種鑒定,用real-time PCR檢測迴腸組織中高遷徙率族蛋白-1(high mobility group box 1,Hmgbl)mRNA錶達,ELISA測定迴腸組織中一氧化氮(nitric oxide,NO)、內皮素-1(endothelin-1,ET-1)濃度,同時觀察胰腺、迴腸組織病理改變,測定迴腸組織濕/榦重比值.結果 ANP組胰腺、迴腸組織損傷明顯,迴腸組織Hmgb1 mRNA的錶達較假手術組上調(P<0.01),NO、ET-1濃度較假手術組升高(P<0.01),分彆為(1.67±0.21)μmol/L、(102.18±9.19)ng/L,細菌移位至肝髒、胰腺、脾髒及腸繫膜淋巴結;治療組迴腸組織中Hmgb1 mRNA的錶達較ANP組下調(P<0.01),NO、ET-1濃度較ANP組降低(P<0.05),分彆為(1.39±0.23)μmol/L、(83.15±5.39)ng/L,迴腸病理損害程度減輕,細菌移位減少.結論 Hmgb1、NO及ET-1濃度在ANP模型大鼠腸道細菌移位中可能具有重要作用.清胰Ⅱ號顆粒劑可能通過下調迴腸組織中Hmgb1 mRNA的錶達,降低NO、ET-1濃度,減輕胰腺、迴腸組織的病理改變,從而抑製腸道細菌移位.
목적 관찰청이Ⅱ호과립제대급성배사성이선염(acute necrotizing pancreatitis,ANP)대서장도세균이위적영향병탐토기궤리.방법 장18지SD대서수궤등분위가수술조、ANP조급치료조,매조6지;ANP조급치료조이30 g/L우광담산납역행주입이담관제작대서ANP모형,조모술후1 h,치료조이250 g/L청이Ⅱ호과립제관위급약(10 mL/kg),6 h 1차,공3차,가수술조급ANP조용동등제량생리염수이상동방식관위.조모후24 h,취대서간장、이선、비장、장계막림파결조직급회장내용물행세균배양급균충감정,용real-time PCR검측회장조직중고천사솔족단백-1(high mobility group box 1,Hmgbl)mRNA표체,ELISA측정회장조직중일양화담(nitric oxide,NO)、내피소-1(endothelin-1,ET-1)농도,동시관찰이선、회장조직병리개변,측정회장조직습/간중비치.결과 ANP조이선、회장조직손상명현,회장조직Hmgb1 mRNA적표체교가수술조상조(P<0.01),NO、ET-1농도교가수술조승고(P<0.01),분별위(1.67±0.21)μmol/L、(102.18±9.19)ng/L,세균이위지간장、이선、비장급장계막림파결;치료조회장조직중Hmgb1 mRNA적표체교ANP조하조(P<0.01),NO、ET-1농도교ANP조강저(P<0.05),분별위(1.39±0.23)μmol/L、(83.15±5.39)ng/L,회장병리손해정도감경,세균이위감소.결론 Hmgb1、NO급ET-1농도재ANP모형대서장도세균이위중가능구유중요작용.청이Ⅱ호과립제가능통과하조회장조직중Hmgb1 mRNA적표체,강저NO、ET-1농도,감경이선、회장조직적병리개변,종이억제장도세균이위.
Objective To observe the effects and mechanism of Qingyi Ⅱ Granules(QYG)on the bacterial translocation in rats with acute necrotizing pancreatitis(ANP).Methods Eighteen Sprague-Dawley rats were randomized equally into 3 groups,the sham-operated group(A),the ANP model group(B)and the treated group(C).Rats in Group B and C were established into ANP model by retrograde injection of 30 g/L sodium taucrocholate into pancreatobiliary duct.QYG was administered,beginning from 1 h after modeling,for three times(every 6 h)per day via intragastric infusion to Group C in dose of 10 mL/kg(250 g/L),while to the other two groups,equal volume of saline was infused instead.All animals were sacrificed 24 h after modeling.The contents in mesenteric lymph nodes and distant sites(liver,spleen,pancreas)were taken for bacterial culture and strain identification,the expression of high mobility group box 1(Hmgb1)mRNA in ileal tissue was assayed by real-time PCR;the levels of nitric oxide(NO)and endothelin-1(ET-1)were determined by ELISA;the wet/dry ratio of ileum was measured;and the pathologic features of pancreas and ileum were examined respectively.Results In Group B,evident pathological injury in pancreas and ileum was shown,expression of Hmgb1 mRNA up-regulated,levels of NO and ET-1 in ileum tissues increased to 1.67±0.21 μmol/L and 102.18±9.19 ng/L respectively,and the bacterial counts in the mesenteric lymph nodes and distant sites increased significantly.Compared with Group B,the level of NO and ET-1 reduced to 1.39±0.23 μmol/L and 83.15±5.39 ng/L,respectively in Group C,with all the above-mentioned abnormal changes alleviated significantly.Conclusion Levels of Hmgb1,NO and ET-1 might play important roles in the ANP model rats with intestinal bacterial translocation.QYG shows effects on preventing the intestinal bacterial translocation by way of down-regulate the Hmgb1 mRNA expression,lowering the concentration of NO and ET,and ameliorating the injury of pancreatic and ileum tissues.
