大连理工大学学报
大連理工大學學報
대련리공대학학보
JOURNAL OF DALIAN UNIVERSITY OF TECHNOLOGY
2009年
6期
812-816
,共5页
王竞%王静%郑春莉%周集体%吕红%曲媛媛
王競%王靜%鄭春莉%週集體%呂紅%麯媛媛
왕경%왕정%정춘리%주집체%려홍%곡원원
硝基苯%盐度%苯酚%苯胺%好氧生物降解
硝基苯%鹽度%苯酚%苯胺%好氧生物降解
초기분%염도%분분%분알%호양생물강해
nitrobenzene%salinity%phenol%aniline%aerobic biodegradation
选取本实验室分离的3株硝基苯高效降解菌--Rhodotorula mucilaginosa Z1、Streptomyces albidoflavus Z2和Micrococcus luteus Z3,将其混合并进行正交实验,得出Z1、Z2、Z3的最佳配比为1 : 3:3,在此基础上构建了混合菌.与单菌相比,混合菌能够在较为苛刻的环境条件下降解硝基苯,并具有较宽的底物范围.同时结合实际硝基苯工业废水特点,考察了混合菌在高盐度下以及苯酚或苯胺和硝基苯共存时对硝基苯的降解.结果表明:混合菌具备较强的耐盐能力,可在5%的高盐(NaCl)条件下有效降解硝基苯;当苯酚或苯胺和硝基苯(200 mg/L)共存,初始浓度分别为100和50 mg/L时,混合菌对硝基苯的降解不受影响.
選取本實驗室分離的3株硝基苯高效降解菌--Rhodotorula mucilaginosa Z1、Streptomyces albidoflavus Z2和Micrococcus luteus Z3,將其混閤併進行正交實驗,得齣Z1、Z2、Z3的最佳配比為1 : 3:3,在此基礎上構建瞭混閤菌.與單菌相比,混閤菌能夠在較為苛刻的環境條件下降解硝基苯,併具有較寬的底物範圍.同時結閤實際硝基苯工業廢水特點,攷察瞭混閤菌在高鹽度下以及苯酚或苯胺和硝基苯共存時對硝基苯的降解.結果錶明:混閤菌具備較彊的耐鹽能力,可在5%的高鹽(NaCl)條件下有效降解硝基苯;噹苯酚或苯胺和硝基苯(200 mg/L)共存,初始濃度分彆為100和50 mg/L時,混閤菌對硝基苯的降解不受影響.
선취본실험실분리적3주초기분고효강해균--Rhodotorula mucilaginosa Z1、Streptomyces albidoflavus Z2화Micrococcus luteus Z3,장기혼합병진행정교실험,득출Z1、Z2、Z3적최가배비위1 : 3:3,재차기출상구건료혼합균.여단균상비,혼합균능구재교위가각적배경조건하강해초기분,병구유교관적저물범위.동시결합실제초기분공업폐수특점,고찰료혼합균재고염도하이급분분혹분알화초기분공존시대초기분적강해.결과표명:혼합균구비교강적내염능력,가재5%적고염(NaCl)조건하유효강해초기분;당분분혹분알화초기분(200 mg/L)공존,초시농도분별위100화50 mg/L시,혼합균대초기분적강해불수영향.
Three effective nitrobenzene degrading strains, Rhodotorula mucilaginosa Z1, Streptomyces albidoflavus Z2 and Micrococcus luteus Z3 were isolated and identified. A defined microbial consortium was constructed through orthogonal experiments by using Z1, Z2 and Z3. The optimal ratio of Z1 to Z2 to Z3 is 1:3 :3. Compared with pure strains, the consortium can degrade nitrobenzene under severe conditions and has a wider substrates range. Considering the characteristics of actual nitrobenzene wastewater, nitrobenzene degradation was investigated by the consortium under high salinity, as well as phenol or aniline co-existence with nitrobenzene. The consortium can degrade nitrobenzene effectively in high salinity of 5% (NaCl). The degradation of nitrobenzene (200 mg/L) by the consortium is not influenced in the presence of 100 mg/L phenol or 50 mg/L aniline.