临床心血管病杂志
臨床心血管病雜誌
림상심혈관병잡지
JOURNAL OF CLINICAL CARDIOLOGY
2009年
11期
809-813
,共5页
杨新玮%张存泰%周洪莲%冯达应%曾秀丽%徐仁德%全小庆%姚震%李天发%钟江华
楊新瑋%張存泰%週洪蓮%馮達應%曾秀麗%徐仁德%全小慶%姚震%李天髮%鐘江華
양신위%장존태%주홍련%풍체응%증수려%서인덕%전소경%요진%리천발%종강화
急性冠状动脉综合征%淋巴细胞%钾离子通道%膜片钳
急性冠狀動脈綜閤徵%淋巴細胞%鉀離子通道%膜片鉗
급성관상동맥종합정%림파세포%갑리자통도%막편겸
acute coronary syndrome%lymphocyte%potassium ion channel%patch clamp
目的:探讨急性冠状动脉综合征(ACS)患者外周血CD_4~+CD28~(null)T细胞和CD_4~+CD28~+T细胞上电压依赖性钾离子通道(Kv1.3)和钙依赖激活的钾离子通道(IKCa1)的表达.方法:入选对象共75例,分为3组:其中ACS组27例,稳定型心绞痛组20例,对照组28例.分离外周血单个核细胞(PBMCs),用ELISA法测定PBMCs中NF-κB的活性,流式细胞仪计数CD_4~+CD28~(null)T细胞占CD4+T细胞的比例.免疫荧光染色标记结合膜片钳技术记录ACS组CD_4~+CD28~(null)T细胞和CD_4~+CD28~+T细胞上Kv1.3和IKCa1的电流,计算单细胞上通道的数目.结果:ACS组NF-κB的活性和CD_4~+CD28~(null)T细胞的比例显著高于稳定型心绞痛组和对照组(P<0.05).NF-κB的活性和CD4+CD28null T细胞的比例具有正相关关系(r=0.369,P<0.05).CD_4~+CD28~(null)T细胞上Kv1.3的数目和电流密度显著高于CD_4~+CD28~+T细胞(P<0.05).CD_4~+CD28~(null)T细胞、CD_4~+CD28~+T细胞上IKCa1的数目差异无统计学意义(P>0.05).结论:Kv1.3通道对CD_4~+CD28~(null)T细胞的功能具有重要作用,Kv1.3通道有可能成为AS治疗的新靶点.
目的:探討急性冠狀動脈綜閤徵(ACS)患者外週血CD_4~+CD28~(null)T細胞和CD_4~+CD28~+T細胞上電壓依賴性鉀離子通道(Kv1.3)和鈣依賴激活的鉀離子通道(IKCa1)的錶達.方法:入選對象共75例,分為3組:其中ACS組27例,穩定型心絞痛組20例,對照組28例.分離外週血單箇覈細胞(PBMCs),用ELISA法測定PBMCs中NF-κB的活性,流式細胞儀計數CD_4~+CD28~(null)T細胞佔CD4+T細胞的比例.免疫熒光染色標記結閤膜片鉗技術記錄ACS組CD_4~+CD28~(null)T細胞和CD_4~+CD28~+T細胞上Kv1.3和IKCa1的電流,計算單細胞上通道的數目.結果:ACS組NF-κB的活性和CD_4~+CD28~(null)T細胞的比例顯著高于穩定型心絞痛組和對照組(P<0.05).NF-κB的活性和CD4+CD28null T細胞的比例具有正相關關繫(r=0.369,P<0.05).CD_4~+CD28~(null)T細胞上Kv1.3的數目和電流密度顯著高于CD_4~+CD28~+T細胞(P<0.05).CD_4~+CD28~(null)T細胞、CD_4~+CD28~+T細胞上IKCa1的數目差異無統計學意義(P>0.05).結論:Kv1.3通道對CD_4~+CD28~(null)T細胞的功能具有重要作用,Kv1.3通道有可能成為AS治療的新靶點.
목적:탐토급성관상동맥종합정(ACS)환자외주혈CD_4~+CD28~(null)T세포화CD_4~+CD28~+T세포상전압의뢰성갑리자통도(Kv1.3)화개의뢰격활적갑리자통도(IKCa1)적표체.방법:입선대상공75례,분위3조:기중ACS조27례,은정형심교통조20례,대조조28례.분리외주혈단개핵세포(PBMCs),용ELISA법측정PBMCs중NF-κB적활성,류식세포의계수CD_4~+CD28~(null)T세포점CD4+T세포적비례.면역형광염색표기결합막편겸기술기록ACS조CD_4~+CD28~(null)T세포화CD_4~+CD28~+T세포상Kv1.3화IKCa1적전류,계산단세포상통도적수목.결과:ACS조NF-κB적활성화CD_4~+CD28~(null)T세포적비례현저고우은정형심교통조화대조조(P<0.05).NF-κB적활성화CD4+CD28null T세포적비례구유정상관관계(r=0.369,P<0.05).CD_4~+CD28~(null)T세포상Kv1.3적수목화전류밀도현저고우CD_4~+CD28~+T세포(P<0.05).CD_4~+CD28~(null)T세포、CD_4~+CD28~+T세포상IKCa1적수목차이무통계학의의(P>0.05).결론:Kv1.3통도대CD_4~+CD28~(null)T세포적공능구유중요작용,Kv1.3통도유가능성위AS치료적신파점.
Objective:To investigate the expression of voltage-gated potassium channel 1.3(Kv1.3) and calcium-activated potassium channel (IKCa1) on the subset of CD4+T lymphocytes in peripheral blood of patients with acute coronary syndrome(ACS). Method:Peripheral blood mononuclear cells (PBMCs) were obtained from 27 patients with ACS, 20 patients with stable angina and 28 matched controls. The proportion of CD_4~+CD28~(null)T lymphocytes was detected by flow cytometry. The activity of nuclear factor kappaB in PBMCs was quantified in an ELISA based essays. To calculate the number of the channel on a single cell the current of Kv1.3 and IKCa1 in T cells were recorded by patch clamp technique combined with immunofluorescence. Result:The proportion of CD_4~+CD28~(null)T lymphocytes and the activity of NF-κB were significantly higher in patients with ACS than in the other groups (P<0.05). There was a positive correlation between the number of CD_4~+CD28~(null)T lymphocyte and the activity of NF-κB(r=0.369,P＜0.05). Patch clamp revealed that the number of IKCa1 in the two subsets of CD4+T cell was similar, Whereas the expression of Kv1.3 appeared to be great different. CD_4~+CD28~(null)T lymphocyte had far more Kv1.3 channels and higher current density than CD_4~+CD28~+T lymphocyte (P<0.05). Conclusion:CD_4~+CD28~(null)T lymphocytes in patients with ACS exhibit Kv1.3 high IKCa1lowphenotype, which may confer the possibility of using Kv1.3 blocker as a new therapy for atherosclerosis.
