色谱
色譜
색보
CHINESE JOURNAL OF CHROMATOGRAPHY
2009年
6期
815-819
,共5页
梁春来%程林丽%沈建忠%张玉洁%张素霞
樑春來%程林麗%瀋建忠%張玉潔%張素霞
량춘래%정림려%침건충%장옥길%장소하
液相色谱-电喷雾串联质谱法%聚醚类药物%鸡肉%鸡肝
液相色譜-電噴霧串聯質譜法%聚醚類藥物%鷄肉%鷄肝
액상색보-전분무천련질보법%취미류약물%계육%계간
liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI MS/MS)%polyether antibiotics%chicken muscle%chicken liver
建立了鸡组织中聚醚类药物多残留检测的高效液相色谱-电喷雾串联质谱方法.采用甲醇提取鸡组织中的拉沙洛菌素、盐霉素、莫能菌素、甲基盐霉素和马杜霉素,经硅胶柱净化,以乙腈(含0.1%甲酸)-0.1%甲酸水溶液(体积比为97∶3)为流动相,Symmetry Shield RP18作为色谱分析柱,多反应监测(MRM)正离子扫描方式进行质谱检测.当5种聚醚类药物的添加水平为鸡肉0.1~1 500 μg/kg、鸡肝0.2~4 500 μg/kg 时,平均回收率为71.6% ~99.1%,日内测定的相对标准偏差(RSD)(n=5)为3.2% ~10.7%,日间RSD(n=3)为4.6% ~14.7% .2种鸡组织中5种聚醚类药物的定量限为0.1~1.0 μg/kg.该方法的灵敏度、准确度和精密度均符合兽药残留分析技术的要求,适用于鸡肉和鸡肝中5种聚醚类药物的多残留检测.
建立瞭鷄組織中聚醚類藥物多殘留檢測的高效液相色譜-電噴霧串聯質譜方法.採用甲醇提取鷄組織中的拉沙洛菌素、鹽黴素、莫能菌素、甲基鹽黴素和馬杜黴素,經硅膠柱淨化,以乙腈(含0.1%甲痠)-0.1%甲痠水溶液(體積比為97∶3)為流動相,Symmetry Shield RP18作為色譜分析柱,多反應鑑測(MRM)正離子掃描方式進行質譜檢測.噹5種聚醚類藥物的添加水平為鷄肉0.1~1 500 μg/kg、鷄肝0.2~4 500 μg/kg 時,平均迴收率為71.6% ~99.1%,日內測定的相對標準偏差(RSD)(n=5)為3.2% ~10.7%,日間RSD(n=3)為4.6% ~14.7% .2種鷄組織中5種聚醚類藥物的定量限為0.1~1.0 μg/kg.該方法的靈敏度、準確度和精密度均符閤獸藥殘留分析技術的要求,適用于鷄肉和鷄肝中5種聚醚類藥物的多殘留檢測.
건립료계조직중취미류약물다잔류검측적고효액상색보-전분무천련질보방법.채용갑순제취계조직중적랍사락균소、염매소、막능균소、갑기염매소화마두매소,경규효주정화,이을정(함0.1%갑산)-0.1%갑산수용액(체적비위97∶3)위류동상,Symmetry Shield RP18작위색보분석주,다반응감측(MRM)정리자소묘방식진행질보검측.당5충취미류약물적첨가수평위계육0.1~1 500 μg/kg、계간0.2~4 500 μg/kg 시,평균회수솔위71.6% ~99.1%,일내측정적상대표준편차(RSD)(n=5)위3.2% ~10.7%,일간RSD(n=3)위4.6% ~14.7% .2충계조직중5충취미류약물적정량한위0.1~1.0 μg/kg.해방법적령민도、준학도화정밀도균부합수약잔류분석기술적요구,괄용우계육화계간중5충취미류약물적다잔류검측.
A liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI MS/MS) method for the determination of 5 polyether antibiotics (lasalocid, salinomycin, mon-ensin, narasin and maduramicin) in chicken tissues was developed. The polyether antibiotics were extracted from chicken tissues with methanol. The extract was evaporated to dry, and re-dissolved in hexane, then cleaned up on a Sep-Pak Silica solid-phase extraction cartridge. The target drugs were eluted with 6 mL methylene chloride-methanol (90= 10, v/v), and the eluate was collected and dried under a gentle stream of nitrogen gas, then the residue was dissolved with 1 mL acetonitrile (containing 0. 1% formic acid) and analyzed by LC-MS/MS. The LC sep-aration was performed on a Symmetry Shield reversed phase C18 bonded silica column with acetonitrile (containing 0. 1% formic acid) - 0. 1% formic acid (97:3, v/v) as mobile phase. The quantification was carried out by positive electrospray ionization and multiple reaction mo-nitoring (MRM) mode. The validation was carried out on spiked chicken muscle (spiked at 0. 1 -1 500 μg/kg) and chicken liver (spiked at 0. 2-4 500 μg/kg), the average recoveries of tar-get drugs ranged from 71. 6%-99. 1% with intra-day relative standard deviations (RSDs) of 3. 2%-10. 7% and inter-day RSDs of 4. 6%-14. 7%. The limits of quantification ( LOQs) in chicken muscle and liver were 0. 1 - 1. 0 μg/kg. The results demonstrated that the sensitivity, accuracy and precision of this method meet the requirements of veterinary drug residue analy-sis. The method is applicable to detect 5 polyether antibiotics in chicken muscle and liver.