CAJ | 학술논문

目的初步探讨骨髓间充质干细胞诱导为神经细胞,及其移植对大鼠脊髓半横断损伤神经功能恢复和运动的影响.方法贴壁培养法分离培养大鼠骨髓间充质干细胞(mesenchymal stem cells,MSCs),大鼠脊髓匀浆上清诱导第3代向神经细胞分化,经免疫组化鉴定分化后细胞的性质.制备大鼠半横断脊髓损伤模型,脊髓损伤局部注射BrdU标记诱导后的神经细胞.细胞移植5周后观察移植细胞在脊髓内存活分布情况.结果倒置显微镜下可见MSCs呈纺锤形和多角形,有1～2个核仁,经脊髓匀浆上清诱导后,发出数个细长突起,并交织成网,诱导后的细胞表达Nestin,可推测诱导后的细胞为MSCs源神经细胞.5周后移植的MSCs在宿主损伤脊髓内聚集并存活,表达MAP-2、NF、GFAP与对照组比较有统计学意义(P<0.05).大鼠运动功能较移植前有所改善.结论 MSCs经脊髓匀浆上清诱导后移植治疗大鼠半横断脊髓损伤可使运动功能得到改善.
목적 초보탐토골수간충질간세포유도위신경세포,급기이식대대서척수반횡단손상신경공능회복화운동적영향.방법 첩벽배양법분리배양대서골수간충질간세포(mesenchymal stem cells,MSCs),대서척수균장상청유도제3대향신경세포분화,경면역조화감정분화후세포적성질.제비대서반횡단척수손상모형,척수손상국부주사BrdU표기유도후적신경세포.세포이식5주후관찰이식세포재척수내존활분포정황.결과 도치현미경하가견MSCs정방추형화다각형,유1～2개핵인,경척수균장상청유도후,발출수개세장돌기,병교직성망,유도후적세포표체Nestin,가추측유도후적세포위MSCs원신경세포.5주후이식적MSCs재숙주손상척수내취집병존활,표체MAP-2、NF、GFAP여대조조비교유통계학의의(P<0.05).대서운동공능교이식전유소개선.결론 MSCs경척수균장상청유도후이식치료대서반횡단척수손상가사운동공능득도개선.
objective To investigate the differentiation of bone marrow-derived mesenchymal stem cells into neurons and transplantation of the stem ceils to repair rat hemisection spinal cord injury.Methods Adherent culture was used to isolate and culture rat bone marrow mesenchymal stem cells(MSCs).The rat spinal cord homogenate supernatant was used to induce neural differentiation of the 3rd generation ceils.The nature of ceil differentiation was identified by immunohistochemistry.The rat model of hemisection spinal cord injury was prepared and BrdU was locally injected to label the induced neurons.The distribution of living cells in the injuried spinal cord was observed at 5 weeks after cell transplantation.Results MSCs were spindle and polygonal,with 1-2 nucleoli seen under the inverted microscope.After induction with spinal cord homogenate supernatant there were a number of slender cytoplasmic projections forming interwined network and showing nestin expression,therefore,indicating the neuronal nature.MSCs at 5 weeks after transplantation into the spinal cord injury were surviving and their expression of MAP-2,NF,GFAP was significantly higher than that in the control rats(P<0.05).The rat motor function was improved than before transplantation.Conclusion MSCs induced by spinal cord homogenate supernatant can be transplanted into hemisection spinal cord injury and improve the motor function of the injuries lesions.