CAJ | 학술논문

建立了柱前衍生-反相高效液相色谱法测定人血白蛋白中辛酸钠含量的方法.用正己烷萃取人血白蛋白中的辛酸钠,与ω-溴苯乙酮和18-冠-6醚在50 ℃反应30 min,然后用甲醇-水(75∶ 25, V/V)于Nova-Park C_(18)柱(150 mm×3.9 mm, 4 μm)上分离,在262 nm检测衍生物,流速为1.0 mL/min,庚酸为内标.辛酸和庚酸的萃取率可分别达到98.2%和97.9%,RSD小于0.9%.方法的线性范围为9.00×10~(-4)～1.44×10~(-2) mol/L(r=0.9995),平均加样回收率为99.7%,RSD≤0.9%.本方法准确、重现性好,可用于人血白蛋白中辛酸钠含量的测定.
건립료주전연생-반상고효액상색보법측정인혈백단백중신산납함량적방법.용정기완췌취인혈백단백중적신산납,여ω-추분을동화18-관-6미재50 ℃반응30 min,연후용갑순-수(75∶ 25, V/V)우Nova-Park C_(18)주(150 mm×3.9 mm, 4 μm)상분리,재262 nm검측연생물,류속위1.0 mL/min,경산위내표.신산화경산적췌취솔가분별체도98.2%화97.9%,RSD소우0.9%.방법적선성범위위9.00×10~(-4)～1.44×10~(-2) mol/L(r=0.9995),평균가양회수솔위99.7%,RSD≤0.9%.본방법준학、중현성호,가용우인혈백단백중신산납함량적측정.
A method was developed for the determination of sodium caprylate in human serum albumin by reversed phase high performance liquid chromatography(RP-HPLC) after pre-column derivatization. The caprylic acid, extracted from human serum albumin by hexane, was treated with ω-bromoacetophenone and 18-crown-6 for 30 min at 50 ℃, and analyzed on a Nova-Park C_(18)(150 mm×3.9 mm, 4 μm) column with methanol-water(75∶ 25, V/V) as the mobile phase. The internal standard was enanthic acid, the flow rate was 1.0 mL/min and the detection wavelength was 262 nm. The extraction yield of caprylic acid was 97.9% and that of enanthic acid was 98.2%. The linear range of sodium caprylate was 9.00×10~(-4)-1.44×10~(-2) mol/L(r=0.9995). The average recovery of caprylic acid was 99.7%, RSD was less than 0.9%. The present method is reliable and relatively simple and can be used for the determination of sodium caprylate in human serum albumin.