CAJ | 학술논문

以钛网电极和铂网电极对培养瓶中大肠杆菌生长过程进行加电刺激,考察其在直流电场作用下的生长情况,并结合盐桥、循环伏安扫描、恒电流等技术对电场作用机理进行研究.结果表明,在电流密度为0.2275mA/cm~2时,直流电场能有效促进大肠杆菌的生长,且随着电流密度的增大,菌体的生长速度逐步加快;当使用盐桥屏蔽电极反应产物时,单纯的离子电流对细胞的生长没有显著影响,水的阴极电解产物是促进微生物生长的主要因素.通过对析氢活性不同的铂网电极与钛网电极加相同电流密度0.0455mA/cm2的电场后,起促进生长作用的主要是阴极电解产物吸附氢和氢气,其中对摇瓶培养的细菌氢气的促进作用显著.
이태망전겁화박망전겁대배양병중대장간균생장과정진행가전자격,고찰기재직류전장작용하적생장정황,병결합염교、순배복안소묘、항전류등기술대전장작용궤리진행연구.결과표명,재전류밀도위0.2275mA/cm~2시,직류전장능유효촉진대장간균적생장,차수착전류밀도적증대,균체적생장속도축보가쾌;당사용염교병폐전겁반응산물시,단순적리자전류대세포적생장몰유현저영향,수적음겁전해산물시촉진미생물생장적주요인소.통과대석경활성불동적박망전겁여태망전겁가상동전류밀도0.0455mA/cm2적전장후,기촉진생장작용적주요시음겁전해산물흡부경화경기,기중대요병배양적세균경기적촉진작용현저.
Growth behavior of Escherichia coli(E.coil) under the exposure to direct current(DC) was investigated with electrolytic stimulation on the culture flask of E.coli using gauze titanium and platinum electrodes.The electric field-activated mechanism was also studied by salt bridge,cyclic voltammestry technique,and constant direct current technique as well as enzyme activity assay of ATPase.The results showed that DC can promote the growth of E.coli under the current density of 0.2275mA/cm~2,and the growth speed is gradually accelerated with increasing current density.When salt bridge electrodes were applied to shield the electrode reactions,the implementation of DC led to neglectable increases in cell growth,and the cathode electrolysis products of water were the primary factor to improve cell growth.The cathode electrolysis products included adsorbed hydrogen and H2 which takes an important role in shaking culture.