CAJ | 학술논문

以马铃薯紫花白品种为试验材料,采用组织培养方法,在离体条件下研究了NaCl胁迫下氮素对马铃薯生理生化特性的影响.主要结果如下:NaCl胁迫下马铃薯叶片脯氨酸含量、叶绿素含量、可溶性蛋白含量及根系活力随氮水平的提高表现为先增加后降低的趋势,在 (N2) 4.17 mmol/L NH4NO3水平下达到最大值,分别为521.21 μg/g、1.96 mg/g、11.08 mg/g、5619.44 μg/(g·h),其中叶绿素含量、可溶性蛋白含量及根系活力在N2水平下显著的高于对照,分别较对照高69.88%、13.07%和59.29%; NaCl胁迫下马铃薯叶片SOD和POD的活性随氮水平的增加逐渐上升,且在 (N3) 6.25 mmol/L NH4NO3水平下达到最大值,其值分别为111.83 U/g和25.467 U/(g·min),较对照增加了37.73%和35.46%.
이마령서자화백품충위시험재료,채용조직배양방법,재리체조건하연구료NaCl협박하담소대마령서생리생화특성적영향.주요결과여하:NaCl협박하마령서협편포안산함량、협록소함량、가용성단백함량급근계활력수담수평적제고표현위선증가후강저적추세,재 (N2) 4.17 mmol/L NH4NO3수평하체도최대치,분별위521.21 μg/g、1.96 mg/g、11.08 mg/g、5619.44 μg/(g·h),기중협록소함량、가용성단백함량급근계활력재N2수평하현저적고우대조,분별교대조고69.88%、13.07%화59.29%; NaCl협박하마령서협편SOD화POD적활성수담수평적증가축점상승,차재 (N3) 6.25 mmol/L NH4NO3수평하체도최대치,기치분별위111.83 U/g화25.467 U/(g·min),교대조증가료37.73%화35.46%.
The effects of nitrogen on physiological and biochemical characteristics of potato cultivar Zihuabai under NaCl stress were studied in culture condition.The results show that, under NaCl stress, the contents of proline, chlorophyll and protein and root system vitality first increase and then decrease with the increase of nitrogen level, and reach the top under 4.17 mmol/L NH4NO3 level.Wherein, the contents of chlorophyll, protein and root system vitality are respectively 69.88%, 13.07% and 59.29% higher than that of the control under 4.17 mmol/L NH4NO3 level; the activities of superoxide dismutase (SOD) and peroxidase (POD) increase generally under NaCl stress with the increase of nitrogen level, and reach the peaks [ 111.83 U/g and 25.467 U/( g·min) ], which are 37.73% and 35.46% higher than that of control, at 6.25 mmol/ LNH4NO3 level.