中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2009年
9期
549-552
,共4页
刘仁臣%夏若虹%程和平%周贞洁
劉仁臣%夏若虹%程和平%週貞潔
류인신%하약홍%정화평%주정길
电磁场%兰尼碱受体钙释放通道%NADH%NADPH氧化还原酶类
電磁場%蘭尼堿受體鈣釋放通道%NADH%NADPH氧化還原酶類
전자장%란니감수체개석방통도%NADH%NADPH양화환원매류
Electromagnetic fields%Ryanodine receptor calcium release channel%NADH,NADPH oxidoreductases
目的 探讨正弦磁场对提取的骨骼肌肌质网(SR)囊泡钙释放通道(RyR1)的影响.方法 采用动态光谱法和同位素标记方法 ,分别检测了正弦磁场对SR囊泡Ca2+释放初速率、[3H]-Ryanodine平衡结合度、还原型辅酶(NADH)的氧化初速率和超氧(O·2-)产率的影响.结果 0.4 mT、50 Hz正弦磁场辐照SR囊泡30 min,由1 mmol/L NADH与1 mmol/L NAD调控的Ca2+释放初速率Con组为(10.82±0.89)pmol·mg-1 pro·s-1、MF组为(14.69±1.21)pmol·mg-1pro·s-1;上调了35%,[3H]-Ryan-odine平衡结合度上调15%,由(2.13±0.05)pmol/mg pro上升到(2.45±0.07)pmol/mg pro.另外,该磁场辐照SR囊泡30 min,导致NADH的氧化速率上调22%,由(0.88±0.11)×10-4FI/s上升到(1.07±0.13)×10-4FI/s;O·2-产率上调32%,由(0.99±0.09)×10-5FI/s上升到(1.31±0.06)×10-5FI/s. 结论 0.4 mT正弦磁场在低氧化还原电势环境下对RyR1有显著的激活,且该磁场促进了NADH氧化酶的活力和O·2-产率的增加.
目的 探討正絃磁場對提取的骨骼肌肌質網(SR)囊泡鈣釋放通道(RyR1)的影響.方法 採用動態光譜法和同位素標記方法 ,分彆檢測瞭正絃磁場對SR囊泡Ca2+釋放初速率、[3H]-Ryanodine平衡結閤度、還原型輔酶(NADH)的氧化初速率和超氧(O·2-)產率的影響.結果 0.4 mT、50 Hz正絃磁場輻照SR囊泡30 min,由1 mmol/L NADH與1 mmol/L NAD調控的Ca2+釋放初速率Con組為(10.82±0.89)pmol·mg-1 pro·s-1、MF組為(14.69±1.21)pmol·mg-1pro·s-1;上調瞭35%,[3H]-Ryan-odine平衡結閤度上調15%,由(2.13±0.05)pmol/mg pro上升到(2.45±0.07)pmol/mg pro.另外,該磁場輻照SR囊泡30 min,導緻NADH的氧化速率上調22%,由(0.88±0.11)×10-4FI/s上升到(1.07±0.13)×10-4FI/s;O·2-產率上調32%,由(0.99±0.09)×10-5FI/s上升到(1.31±0.06)×10-5FI/s. 結論 0.4 mT正絃磁場在低氧化還原電勢環境下對RyR1有顯著的激活,且該磁場促進瞭NADH氧化酶的活力和O·2-產率的增加.
목적 탐토정현자장대제취적골격기기질망(SR)낭포개석방통도(RyR1)적영향.방법 채용동태광보법화동위소표기방법 ,분별검측료정현자장대SR낭포Ca2+석방초속솔、[3H]-Ryanodine평형결합도、환원형보매(NADH)적양화초속솔화초양(O·2-)산솔적영향.결과 0.4 mT、50 Hz정현자장복조SR낭포30 min,유1 mmol/L NADH여1 mmol/L NAD조공적Ca2+석방초속솔Con조위(10.82±0.89)pmol·mg-1 pro·s-1、MF조위(14.69±1.21)pmol·mg-1pro·s-1;상조료35%,[3H]-Ryan-odine평형결합도상조15%,유(2.13±0.05)pmol/mg pro상승도(2.45±0.07)pmol/mg pro.령외,해자장복조SR낭포30 min,도치NADH적양화속솔상조22%,유(0.88±0.11)×10-4FI/s상승도(1.07±0.13)×10-4FI/s;O·2-산솔상조32%,유(0.99±0.09)×10-5FI/s상승도(1.31±0.06)×10-5FI/s. 결론 0.4 mT정현자장재저양화환원전세배경하대RyR1유현저적격활,차해자장촉진료NADH양화매적활력화O·2-산솔적증가.
Objective To investigate the effects of sinusoidal magnetic field on isolated sarcoplas-mic reticulum (SR) calcium release channel (RyR1) function. Methods With the Ca2+ dynamic spectrum and isotope labeled methods, the Ca2+ release and [3H]-Ryanodine binding, the initial rates of NADH oxida-tion and the production of superoxide of SR exposed to 50 Hz sinusoidal magnetic field (MF) were investi-gated respectively. Results 0.4 mT, 50 Hz sinusoidal MF exposure for 30 min increased SR Ca2+ release initial rate about 35% from (10.82±0.89)pmol· mg-1 pro·s-1 to (14.69±1.21)pmol·mg-1 pro·s-1; and the [3H]-Ryanodine binding by about 15% from (2.13±0.05)pmol/mg pro to (2.45±0.07)pmol/mg pro, which regu-lated by 1 mmol/L NADH with 1 mmol/L NAD+. Meanwhile MF upregnlated the rate of NADH oxidation by about 22% from (0.88±0.11)×10-4 FI/s to (1.07±0.13)×10-4 FI/s and upregulated the production of super-oxide by about 32% from (0.99±0.09)×10-5 FI/s to (1.31±0.06)×10-5 FI/s. Conclusion 0.4 mT sinu-soidal MF increases the activity of RyR1 within the low redox potential environment, and promotes NADH oxidase activity and superoxide production.
