中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2010年
5期
397-400
,共4页
姜晓丹%于立坚%廖铭能%于廷曦
薑曉丹%于立堅%廖銘能%于廷晞
강효단%우립견%료명능%우정희
川芎嗪%抗抑郁样作用%强迫游泳%慢性应激抑郁模型%大鼠
川芎嗪%抗抑鬱樣作用%彊迫遊泳%慢性應激抑鬱模型%大鼠
천궁진%항억욱양작용%강박유영%만성응격억욱모형%대서
Tetramethylpyrazine (TMP)%Antidepressive-like activity%Forced-swimming test%Chronic mild stress (CMS) animal model of depression%Rats
目的 探讨川芎嗪的抗抑郁作用.方法 采用强迫游泳实验制备急性应激大鼠抑郁模型,长期轻度应激+孤养制备大鼠慢性抑郁模型.慢性应激实验采用SD雄性大鼠,基于糖水摄入实验结果随机分为对照组、模型(慢性应激)组、阳性对照(慢性应激+氟西汀)组和川芎嗪(慢性应激+不同剂量川芎嗪)组,每组13或14只动物.氟西汀(2.0mg·kg-1·d-1)和川芎嗪灌胃(10,20,40mg·kg-1·d-1),持续28d.对照组不予任何刺激.慢性应激期间测定动物的体质量和摄食量,旷场行为、液体消耗试验检查动物行为和液体消耗(糖水偏爱)的变化,实验末行强迫游泳实验.结果 短期应用川芎嗪组大鼠强迫游泳(急性抑郁)不动时间[(89.0±37.0)s]明显缩短[对照组为(117.1±32.1)s,P<0.05].川芎嗪部分逆转慢性应激引起的动物的抑郁表现.实验第4周末,与模型组比,川芎嗪(40mg/kg×28)组液体总消耗和糖水消耗量显著增加(P<0.05),糖水偏爱百分比升高,水平运动得分增加,慢性抑郁期强迫游泳不动时间[(91.9±31.5) s]显著缩短[模型组为(124.4±27.0)s,P<0.05].结论 川芎嗪有明显的抗抑郁样作用.
目的 探討川芎嗪的抗抑鬱作用.方法 採用彊迫遊泳實驗製備急性應激大鼠抑鬱模型,長期輕度應激+孤養製備大鼠慢性抑鬱模型.慢性應激實驗採用SD雄性大鼠,基于糖水攝入實驗結果隨機分為對照組、模型(慢性應激)組、暘性對照(慢性應激+氟西汀)組和川芎嗪(慢性應激+不同劑量川芎嗪)組,每組13或14隻動物.氟西汀(2.0mg·kg-1·d-1)和川芎嗪灌胃(10,20,40mg·kg-1·d-1),持續28d.對照組不予任何刺激.慢性應激期間測定動物的體質量和攝食量,曠場行為、液體消耗試驗檢查動物行為和液體消耗(糖水偏愛)的變化,實驗末行彊迫遊泳實驗.結果 短期應用川芎嗪組大鼠彊迫遊泳(急性抑鬱)不動時間[(89.0±37.0)s]明顯縮短[對照組為(117.1±32.1)s,P<0.05].川芎嗪部分逆轉慢性應激引起的動物的抑鬱錶現.實驗第4週末,與模型組比,川芎嗪(40mg/kg×28)組液體總消耗和糖水消耗量顯著增加(P<0.05),糖水偏愛百分比升高,水平運動得分增加,慢性抑鬱期彊迫遊泳不動時間[(91.9±31.5) s]顯著縮短[模型組為(124.4±27.0)s,P<0.05].結論 川芎嗪有明顯的抗抑鬱樣作用.
목적 탐토천궁진적항억욱작용.방법 채용강박유영실험제비급성응격대서억욱모형,장기경도응격+고양제비대서만성억욱모형.만성응격실험채용SD웅성대서,기우당수섭입실험결과수궤분위대조조、모형(만성응격)조、양성대조(만성응격+불서정)조화천궁진(만성응격+불동제량천궁진)조,매조13혹14지동물.불서정(2.0mg·kg-1·d-1)화천궁진관위(10,20,40mg·kg-1·d-1),지속28d.대조조불여임하자격.만성응격기간측정동물적체질량화섭식량,광장행위、액체소모시험검사동물행위화액체소모(당수편애)적변화,실험말행강박유영실험.결과 단기응용천궁진조대서강박유영(급성억욱)불동시간[(89.0±37.0)s]명현축단[대조조위(117.1±32.1)s,P<0.05].천궁진부분역전만성응격인기적동물적억욱표현.실험제4주말,여모형조비,천궁진(40mg/kg×28)조액체총소모화당수소모량현저증가(P<0.05),당수편애백분비승고,수평운동득분증가,만성억욱기강박유영불동시간[(91.9±31.5) s]현저축단[모형조위(124.4±27.0)s,P<0.05].결론 천궁진유명현적항억욱양작용.
Objective To study the potential antidepressive-like effect of tetramethylpyrazine (TMP). Methods Forced-swimming and chronic mild stress (CMS) tests were performed to assess the antidepressant-like activity of TMP. Male Sprague-Dawley (SD) strain rats were divided into six matched groups (n= 13 or 14 in each group) based on their sucrose consumption:control, CMS, CMS + fluoxetine, and CMS + TMP groups. The rats except control were housed separately in different rooms, and the rat model of depression was established by exposing to an unpredictable sequence of stressors for 28 days; the rats in CMS + fluoxetine were exposed to CMS and received administration of FLU (2.0 mg· kg-1·d-1 ,ig) for 28 days; the rats in CMS + TMP groups were exposed to CMS and received administration of TMP (10,20,40 mg·kg-1·d-1 ,ig) , respectively, for 28 days. The rats in control group were given ordinary daily care and received ig administration of normal saline simultaneously. The body weight, food intake and fluid consumption were measured, and the behaviors were examined by open field during the duration of the stress procedure, and forced-swimming test was performed 1 day after last unpredictable stressor. Results Acute administration of TMP markedly decreased the duration of immobility during forced-swimming test((89.0 ±37.0)s vs (117.1 ±32. 1)s, P<0.05) . Chronic administration of TMP partially countered the effects of CMS on consumption of sucrose solution and locomotion and exploration behavior, and potently shortened the immobility time during forced-swimming test following CMS in rats. The results showed that long-term administration of TMP partially reversed the effects of CMS on the body weight gain,the consumption of sucrose solution,the squares crossing in open field test and the immobility time during forced-swimming test in rats ((91.9 ±31.5) vs (124.4±27.0)s,P<0.05).Conclusion TMP shows obvious antidepressant-like activity.
