CAJ | 학술논문

目的观察七氟烷对小鼠短期内记忆能力及其对海马突触传递效能的影响.方法昆明小鼠吸入七氟烷后12h、24h、72h,分别用Y型迷宫测试进行自发交替和活动能力,离体脑片细胞外记录技术观测海马短时程增强(STP)的变化.结果与对照组比较,吸入1.5％七氟烷后12h可使小鼠的自发交替的百分率明显降低[(54.7±1.7)％,P＜0.01],活动能力明显下降(16.4±1.3,P＜0.01),在海马脑片上STP诱发后群峰电位(PS)幅值明显降低[(122.3±13.9)％,P＜0.05],但在吸入七氟烷后24h和72 h均无影响.在离体海马脑片直接应用七氟烷后,可使基础[(83.5±8.9)％,P＜0.01)]和强直后[(116.5±14.9)％,P＜0.01)]PS幅值降低,但冲洗后PS幅度可恢复.结论七氟烷在短期内可明显抑制突触传递效能,损害记忆功能,但无远期作用.
목적 관찰칠불완대소서단기내기억능력급기대해마돌촉전체효능적영향.방법 곤명소서흡입칠불완후12h、24h、72h,분별용Y형미궁측시진행자발교체화활동능력,리체뇌편세포외기록기술관측해마단시정증강(STP)적변화.결과 여대조조비교,흡입1.5％칠불완후12h가사소서적자발교체적백분솔명현강저[(54.7±1.7)％,P＜0.01],활동능력명현하강(16.4±1.3,P＜0.01),재해마뇌편상STP유발후군봉전위(PS)폭치명현강저[(122.3±13.9)％,P＜0.05],단재흡입칠불완후24h화72 h균무영향.재리체해마뇌편직접응용칠불완후,가사기출[(83.5±8.9)％,P＜0.01)]화강직후[(116.5±14.9)％,P＜0.01)]PS폭치강저,단충세후PS폭도가회복.결론 칠불완재단기내가명현억제돌촉전체효능,손해기억공능,단무원기작용.
Objective To investigate the effects of sevoflurane on short-term memory impairment and the relative synaptic mechanism.Methods At 12 h,24 h and 72 h after the mice were exposed to 1.5％ sevoflurane for 1h,the spontaneous alternation and locomotor activity was assessed by Y maze,the short term potentiation (STP) were measured with extracellular recording technique in hippocampal slices.Results At 12h after administration with sevoflurane in vivo,the spontaneous alternation ( (54.7 ± 1.7) ％,P＜0.01 ) and locomotor activity (16.4 ± 1.3,P ＜ 0.01 ) decreased significantly compared with that of control group,and the population spike amplitude after induction of STP ( ( 122.3 ± 13.9) ％,P ＜ 0.05 ) decreased significantly in hippocampal slices,but there was no different at 24h and 72h.After administration with sevoflurane in vitro,the basic ( ( 83.5 ± 8.9) ％,P ＜ 0.01 ) or titanic ( ( 116.5 ± 14.9) ％,P ＜ 0.01 ) population spike amplitude decreased significantly in hippocampal slices,but the amplitude could recovery after wash-out.Conclusion Sevoflurane can impair the shortterm memory by suppressing synaptic transmission in the near future but not the long future.