中华结核和呼吸杂志
中華結覈和呼吸雜誌
중화결핵화호흡잡지
Chinese Journal of Tuberculosis and Respiratory Diseases
2012年
8期
596-600
,共5页
丁光贵%张国良%陈心春%张明霞%杨林%王正
丁光貴%張國良%陳心春%張明霞%楊林%王正
정광귀%장국량%진심춘%장명하%양림%왕정
白细胞介素类%多态性,单核苷酸%结核,肺%疾病遗传易感性
白細胞介素類%多態性,單覈苷痠%結覈,肺%疾病遺傳易感性
백세포개소류%다태성,단핵감산%결핵,폐%질병유전역감성
Interleukins%Polymorphism,single nucleotide%Tuberculosis,pulmonary%Genetic predisposition to disease
目的 探讨IL-22基因单核苷酸多态性(SNP)与肺结核易感性的关系.方法 选取2009年1月至2010年12月深圳市第三人民医院收治并确诊的肺结核患者479例为病例组,其中男212例,女267例；年龄18～ 69岁,平均(36±17)岁；对照组为同期在该医院体检者358名,其中男162名,女196名,年龄18～60岁,平均(34±13)岁.应用MassARRAY时间飞行质谱生物芯片技术,检测IL-22基因6个SNP位点(rs1182844、rs2227473、rs2227476、rs2227480、rs2227485和rs2227508)基因型,对SNP位点进行Hardy-Weiberg平衡检验,并计算各SNP位点等位基因频率及比值比.选取rs2227473不同基因型肺结核患者外周血单个核细胞(PBMC),使用抗人CD3和CD28抗体联合刺激后,采用ELISA法测定不同基因型患者细胞上清液中IL-22浓度.分别采用x2检验和t检验对两组SNP等位基因频率和IL-22浓度进行统计学分析.结果 病例组和对照组6个IL-22基因SNP位点的基因型分布均处于遗传平衡状态.其中rs2227473位点存在GG、GA和AA基因型,病例组仅有等位基因G频率(90.0％,862/958)显著高于对照组(85.6％,613/716),差异有统计学意义(x2=7.448,P ＜0.01,OR=1.509,95％CI-1.121～2.030).rs2227473位点GG基因型的PBMC培养上清液中IL-22浓度为(19 ±5) ng/L,显著低于AA/GA基因型的(39±6)ng/L,差异有统计学意义(t=2.686,P＜0.01).结论 IL22基因rs2227473多态性与肺结核易感性有显著相关性,其等位基因G是肺结核的易感基因.rs2227473多态性影响宿主PBMC的IL-22表达水平,在调节肺结核保护性免疫过程起重要作用.
目的 探討IL-22基因單覈苷痠多態性(SNP)與肺結覈易感性的關繫.方法 選取2009年1月至2010年12月深圳市第三人民醫院收治併確診的肺結覈患者479例為病例組,其中男212例,女267例；年齡18～ 69歲,平均(36±17)歲；對照組為同期在該醫院體檢者358名,其中男162名,女196名,年齡18～60歲,平均(34±13)歲.應用MassARRAY時間飛行質譜生物芯片技術,檢測IL-22基因6箇SNP位點(rs1182844、rs2227473、rs2227476、rs2227480、rs2227485和rs2227508)基因型,對SNP位點進行Hardy-Weiberg平衡檢驗,併計算各SNP位點等位基因頻率及比值比.選取rs2227473不同基因型肺結覈患者外週血單箇覈細胞(PBMC),使用抗人CD3和CD28抗體聯閤刺激後,採用ELISA法測定不同基因型患者細胞上清液中IL-22濃度.分彆採用x2檢驗和t檢驗對兩組SNP等位基因頻率和IL-22濃度進行統計學分析.結果 病例組和對照組6箇IL-22基因SNP位點的基因型分佈均處于遺傳平衡狀態.其中rs2227473位點存在GG、GA和AA基因型,病例組僅有等位基因G頻率(90.0％,862/958)顯著高于對照組(85.6％,613/716),差異有統計學意義(x2=7.448,P ＜0.01,OR=1.509,95％CI-1.121～2.030).rs2227473位點GG基因型的PBMC培養上清液中IL-22濃度為(19 ±5) ng/L,顯著低于AA/GA基因型的(39±6)ng/L,差異有統計學意義(t=2.686,P＜0.01).結論 IL22基因rs2227473多態性與肺結覈易感性有顯著相關性,其等位基因G是肺結覈的易感基因.rs2227473多態性影響宿主PBMC的IL-22錶達水平,在調節肺結覈保護性免疫過程起重要作用.
목적 탐토IL-22기인단핵감산다태성(SNP)여폐결핵역감성적관계.방법 선취2009년1월지2010년12월심수시제삼인민의원수치병학진적폐결핵환자479례위병례조,기중남212례,녀267례；년령18～ 69세,평균(36±17)세；대조조위동기재해의원체검자358명,기중남162명,녀196명,년령18～60세,평균(34±13)세.응용MassARRAY시간비행질보생물심편기술,검측IL-22기인6개SNP위점(rs1182844、rs2227473、rs2227476、rs2227480、rs2227485화rs2227508)기인형,대SNP위점진행Hardy-Weiberg평형검험,병계산각SNP위점등위기인빈솔급비치비.선취rs2227473불동기인형폐결핵환자외주혈단개핵세포(PBMC),사용항인CD3화CD28항체연합자격후,채용ELISA법측정불동기인형환자세포상청액중IL-22농도.분별채용x2검험화t검험대량조SNP등위기인빈솔화IL-22농도진행통계학분석.결과 병례조화대조조6개IL-22기인SNP위점적기인형분포균처우유전평형상태.기중rs2227473위점존재GG、GA화AA기인형,병례조부유등위기인G빈솔(90.0％,862/958)현저고우대조조(85.6％,613/716),차이유통계학의의(x2=7.448,P ＜0.01,OR=1.509,95％CI-1.121～2.030).rs2227473위점GG기인형적PBMC배양상청액중IL-22농도위(19 ±5) ng/L,현저저우AA/GA기인형적(39±6)ng/L,차이유통계학의의(t=2.686,P＜0.01).결론 IL22기인rs2227473다태성여폐결핵역감성유현저상관성,기등위기인G시폐결핵적역감기인.rs2227473다태성영향숙주PBMC적IL-22표체수평,재조절폐결핵보호성면역과정기중요작용.
Objective To study the association between single nucleotide polymorphisms (SNPs) of interleukin-22 (IL-22) gene and pulmonary tuberculosis.Methods From January 2009 to December 2010,clinical data of 479 patients with pulmonary tuberculosis in Shenzhen Third People' s Hospital were collected.There were 212 males,and 267 females,aging from 18 to 69 (mean 36 ± 17) years,as well as 358 healthy controls (162 males and 196 females),aging from 18 to 60 (mean 34 ± 13 ) years.The genotype of SNPs (rs1182844,rs2227473,rs2227476,rs2227480,rs2227485,rs2227508) in IL-22 gene were determined with MassARRAY assay,after Hardy-Weinberg equilibrium test,and the allelic frequency and odds ratio were calculated. Peripheral blood mononuclear cells (PBMCs) from patients with different rs2227473 genotypes were stimulated with anti-CD3 and CD28, and then the IL-22 concentration in supernatant was determined with ELSIA.The SNP allelic frequencies between 2 groups were analyzed by chisquare and IL-22 concentration by t-test. Results The frequency of allele G of rs2227473 SNP was significantly higher in tuberculosis group than that in the control group ( x2 ＝ 7.448,P ＜ 0.01,OR =1.509,95％CI =1.121 -2.030). The other 5 SNPs allele frequency were not statistically siguificant between the 2 groups ( x2 =O.528 - 3.571,all P ＞ 0.05 ).The secretion of IL-22 was significantly lower in PBMCs with genotype GG of rs2227473 SNP as compared to that in the others (GA/AA) (t =2.686,P ＜0.01 ).Conclusions The results suggested that the rs2227473 SNP in IL-22 was associated with the risk of pulmonary tuberculosis.The allele G was the risk factor of pulmonary tuberculosis.The SNP ( rs2227473 )may play an important role in the protective immune process against tuberculosis by affecting the IL-22 expression of PBMCs.
