中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2010年
5期
359-362
,共4页
黄鹏%王春友%吴河水%熊炯炘
黃鵬%王春友%吳河水%熊炯炘
황붕%왕춘우%오하수%웅형흔
胰腺肿瘤%Notch信号%肿瘤干细胞%增殖%分化
胰腺腫瘤%Notch信號%腫瘤榦細胞%增殖%分化
이선종류%Notch신호%종류간세포%증식%분화
Pancreatic neoplasms%Notch signaling%Tumor stem cells%Proliferation%Differentiation
目的 探讨激活的Notch1信号系统对PANC-1胰腺癌干细胞增殖与分化的调控.方法 向体外培养的胰腺癌干细胞中分别加入Notch1激活剂rhNF-κB和抑制剂MW167,空白对照加PBS缓冲液.RT-PCR、Western印迹和流式细胞术等方法 检测Notch1信号系统的表达,及加入激活剂rhNF-κB和抑制剂MW167后,CD44和CD24的表达,以及细胞周期的变化.结果 胰腺癌干细胞中Notch信号系统的4个受体只有Notch1表达,配体只有JAG1表达;对照组的S期和G2期为21.5%和12.7%,使用MW167诱导时,细胞周期的S期和G2期所占的百分比降为17.2%和10.5%,干细胞所表达的CD44和CD24亦显著降低(P<0.05或P<0.01);而使用rhNF-κB诱导,则S期和G2期所占的百分比显著增高为29.3%和15.2%,同时CD44和CD24表达也增高(P<0.05或P<0.01).结论 当Notch信号系统被激活时,胰腺癌干细胞进行增殖;当Notch信号系统被抑制时,胰腺癌干细胞进行分化.
目的 探討激活的Notch1信號繫統對PANC-1胰腺癌榦細胞增殖與分化的調控.方法 嚮體外培養的胰腺癌榦細胞中分彆加入Notch1激活劑rhNF-κB和抑製劑MW167,空白對照加PBS緩遲液.RT-PCR、Western印跡和流式細胞術等方法 檢測Notch1信號繫統的錶達,及加入激活劑rhNF-κB和抑製劑MW167後,CD44和CD24的錶達,以及細胞週期的變化.結果 胰腺癌榦細胞中Notch信號繫統的4箇受體隻有Notch1錶達,配體隻有JAG1錶達;對照組的S期和G2期為21.5%和12.7%,使用MW167誘導時,細胞週期的S期和G2期所佔的百分比降為17.2%和10.5%,榦細胞所錶達的CD44和CD24亦顯著降低(P<0.05或P<0.01);而使用rhNF-κB誘導,則S期和G2期所佔的百分比顯著增高為29.3%和15.2%,同時CD44和CD24錶達也增高(P<0.05或P<0.01).結論 噹Notch信號繫統被激活時,胰腺癌榦細胞進行增殖;噹Notch信號繫統被抑製時,胰腺癌榦細胞進行分化.
목적 탐토격활적Notch1신호계통대PANC-1이선암간세포증식여분화적조공.방법 향체외배양적이선암간세포중분별가입Notch1격활제rhNF-κB화억제제MW167,공백대조가PBS완충액.RT-PCR、Western인적화류식세포술등방법 검측Notch1신호계통적표체,급가입격활제rhNF-κB화억제제MW167후,CD44화CD24적표체,이급세포주기적변화.결과 이선암간세포중Notch신호계통적4개수체지유Notch1표체,배체지유JAG1표체;대조조적S기화G2기위21.5%화12.7%,사용MW167유도시,세포주기적S기화G2기소점적백분비강위17.2%화10.5%,간세포소표체적CD44화CD24역현저강저(P<0.05혹P<0.01);이사용rhNF-κB유도,칙S기화G2기소점적백분비현저증고위29.3%화15.2%,동시CD44화CD24표체야증고(P<0.05혹P<0.01).결론 당Notch신호계통피격활시,이선암간세포진행증식;당Notch신호계통피억제시,이선암간세포진행분화.
Objective To study regulation of the differentiation and proliferation of stem cells of pancreatic adenocarcinoma by activated Notch 1 signaling.Methods The rhNF-kB,an activator of Notch signaling,and the γ-secretase inhibitor Ⅱ(MW167)were added into the mediums of tumor stem cells of pancreatic adenocarcinoma respectively,and the control was added with PBS buffer.Then notch signaling was measured by RT-PCR.After intervention with rhNF-kB and MW 167,cell cycle,CD44 and CD24 were detected by Western blot and flow cytometry.Results Notch 1 and JAG 1 were expressed in stem cells of pancreatic adenocarcinoma.In the control group,21.5%and 12.7%of cells stayed at S and G2 phase.However,it decreased to 17.2%and 10.5%in MW167 group,29.3%and 15.2%in rhNF-κB group(P<0.05 or P<0.01).The expression of CD44 and CD24 of rhNF-κB group was higher than that of control group,and the effect of promoting proliferation was obvious.In contrast,the expression of CD44 and CD24 of MW167 group was decreased apparently (P<0.05 or P<0.01).Conclusion When Notch signaling is activated,the stem cells of pancreatic adenocarcinoma go on proliferating.On the contrary,the cells go on differentiating when Notch signaling is suppressed.
