CAJ | 학술논문

目的探讨不同海拔地区不同年龄组人外周血白细胞端粒DNA长度变化.方法分别选取高原组(花石峡海拔4380 m)与平原组(北京海拔43 m)健康人外周血标本172份.其中高原组95名(男性47名,女性48名),平原组77名(男性39名,女性38名).两组年龄分布基本相同.实验采用Southern杂交方法检测其端粒限制性片段长度(terminal restriction fragment,TRF),邻苯三酚氧化法测定超氧化物歧化酶(SOD),硫代巴比妥(TBA)比色法测定丙二醛(MDA)并做统计学分析.结果高原组与平原组健康人平均TRF检测结果,高原组男性TRF值为10.45±1.35,女性为10.50±1.45;平原组男性TRF值为11.29±1.10,女性为11.31±1.13;高原组与平原组男性与女性TRF.值均与年龄呈负相关性;对高原组与平原组分别进行男性与女性4个不同年龄段间方差分析,4个年龄段均数间进行比较发现无论男性或女性,高原组与平原组25及55岁组比较,差异有统计学意义.25～44岁和45～65岁高原组和平原组相同年龄段组SOD,MDA测定值相比较,差异有统计学意义.结论高原组人FRF均随年龄增长而逐渐缩短,TRF与年龄间存在负相关关系;高原组与平原组不同性别TRF不同,高原组TRF水平低于平原组;随着年龄的增长高原组TRF缩短更明显.
목적 탐토불동해발지구불동년령조인외주혈백세포단립DNA장도변화.방법 분별선취고원조(화석협해발4380 m)여평원조(북경해발43 m)건강인외주혈표본172빈.기중고원조95명(남성47명,녀성48명),평원조77명(남성39명,녀성38명).량조년령분포기본상동.실험채용Southern잡교방법검측기단립한제성편단장도(terminal restriction fragment,TRF),린분삼분양화법측정초양화물기화매(SOD),류대파비타(TBA)비색법측정병이철(MDA)병주통계학분석.결과 고원조여평원조건강인평균TRF검측결과,고원조남성TRF치위10.45±1.35,녀성위10.50±1.45;평원조남성TRF치위11.29±1.10,녀성위11.31±1.13;고원조여평원조남성여녀성TRF.치균여년령정부상관성;대고원조여평원조분별진행남성여녀성4개불동년령단간방차분석,4개년령단균수간진행비교발현무론남성혹녀성,고원조여평원조25급55세조비교,차이유통계학의의.25～44세화45～65세고원조화평원조상동년령단조SOD,MDA측정치상비교,차이유통계학의의.결론 고원조인FRF균수년령증장이축점축단,TRF여년령간존재부상관관계;고원조여평원조불동성별TRF불동,고원조TRF수평저우평원조;수착년령적증장고원조TRF축단경명현.
Objective To investigate the relationship between the length of telomere DNA and ageat different altitude areas. Methods A1l 172 peripheral blood samples were randomly selected from healthy individuals of different ages from 25 to 65 years old. High altitude group (47 males, 48 females) living at 8.11altitude of4380 m (HA group), sea level group (39 males, 38 females) living at all altitude of43 m (SL group). r11le terminal restriction fragment (TRF) length of telomere DNA was measured by Southern blotting analysis. The plasma levels of malondialdehyde(MDA) and superoxide dismutase (SOD) wereassayed. Results Average TRF lengths of males and females in HA groups were 10.45 4-1.35 and 10.50 4-1.45.Average TRF lengths of males and females in SL groups were 11.29±1.10 and 11.31 4-1.13.Anegative correlation Was shown between the average TRF length and age of males in two groups(P<0.01).This was also the case forfemale8.ANOVA test Was used to analyze the difference between TRF length and gender at different ages(P<0.001).It was shown that there Was significant difference in TRF length between the male(25 years old and 55 years old)and female(25 years old and 55 years 0ld)in two groups at different ages(P<0.05).The plasma levels of SOD and MDA were significant different between HA groups and SL groups(25-44 years old groups /45-65 years old groups)(P<0.05). Conclusion Obviously shortening of telomere was observed by increasing of ages in high altitude groups. There wasa negative correlation between the length of telomere DNA and ages. Telomere shortening became more obviously in high altitude group than in sea level group in keeping with the age increases.