CAJ | 학술논문

目的探讨抑癌基因FBXW7在Notch1诱导的小鼠白血病发展中的表达变化规律.方法采用Notch1过表达小鼠急性T淋巴细胞白血病移植模型,在发病不同阶段分离骨髓单个核细胞,并在发病晚期用流式细胞术分选CD45.2+GFP+白血病细胞.实时定量PCR方法检测FBXW7的表达变化.结果对照组和白血病组小鼠骨髓细胞均表达FBXW7.Notch1过表达导致的小鼠白血病发展过程中,FBXW7在对照小鼠中表达水平逐渐升高；而在白血病小鼠中,随着白血病发展,表达水平逐渐下降,第12天降至对照组的1/6.分选后的CD45.2+GFP+白血病细胞低表达FBXW7.结论 FBXW7在小鼠白血病模型中低表达,提示FBXW7介导的泛素化降解途径异常可能与Notch1诱导小鼠白血病发生、发展相关.
목적 탐토억암기인FBXW7재Notch1유도적소서백혈병발전중적표체변화규률.방법 채용Notch1과표체소서급성T림파세포백혈병이식모형,재발병불동계단분리골수단개핵세포,병재발병만기용류식세포술분선CD45.2+GFP+백혈병세포.실시정량PCR방법검측FBXW7적표체변화.결과 대조조화백혈병조소서골수세포균표체FBXW7.Notch1과표체도치적소서백혈병발전과정중,FBXW7재대조소서중표체수평축점승고；이재백혈병소서중,수착백혈병발전,표체수평축점하강,제12천강지대조조적1/6.분선후적CD45.2+GFP+백혈병세포저표체FBXW7.결론 FBXW7재소서백혈병모형중저표체,제시FBXW7개도적범소화강해도경이상가능여Notch1유도소서백혈병발생、발전상관.
Objective To investigate the expression of FBXW7 during the development of Notch1induced murine leukemia.Methods Notch1 over-expressing murine model of T-cell acute lymphoblastic leukemia was used to study the expression of FBXW7 by real-time PCR methods.Bone marrow mononuclear cells (BMNC) were isolated on different days after transplantation and CD45.2+ GFP+ leukemia cells were sorted by flow cytometry at late stage.The expression changes of FBXW7 were tested by real-time PCR.Results The mouse bone marrow cells both from leukemia and control groups expressed FBXW7.Different expression patterns of FBXW7 were observed during the development of leukemia. The expression of FBXW7 was gradually increased in control group, whereas the expression level of FBXW7 in leukemia group was decreased steadily and reached one-sixth of that in control group on 12th day.Furthermore,lower expression level of FBXW7 was observed in CD45+.2 GFP+ leukemia cells.Conclusion Decreased expression of FBXW7 is observed in Notch1-induced mouse leukemia model,suggesting that the abnormal ubiquitin degradation pathway mediated by FBXW7 might contribute to the leukemogenesis in Notch1-induced murine leukemia model.