中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2010年
12期
1086-1089
,共4页
孔朝红%刘煜敏%朱江%周绍霞
孔朝紅%劉煜敏%硃江%週紹霞
공조홍%류욱민%주강%주소하
内皮祖细胞%慢性脑缺血%水迷宫%血管再生%血管内皮生长因子
內皮祖細胞%慢性腦缺血%水迷宮%血管再生%血管內皮生長因子
내피조세포%만성뇌결혈%수미궁%혈관재생%혈관내피생장인자
Endothelial progenitor cells%Chronic cerebral ischemic%Morris water maze%angiogenesis%Vascular endothelial growth factor
目的 探讨自体移植内皮祖细胞对慢性脑缺血大鼠行为学习功能的影响.方法 制作慢性脑缺血大鼠模型,静脉自体移植内皮祖细胞,进行水迷宫检测学习记忆功能,并从脑缺血区新生细胞、脑部血管、血管内皮生长因子(VEGF)等方面探讨可能的机制.结果 ①Morris水迷宫实验中,训练第2～5天的逃避潜伏期实验组(EPC组)[分别为(44.45 ±9.44)s,(38.32±1.51)s,(34.95±6.76)s,(24.46±5.47)s明显短于对照组(PBS组)[对应为(52.79 ±6.47)s,(43.15±11.21)s,(50.29±11.41)s,(53.75±7.35)s](P＜0.01);第一象限游泳时间EPC组(26.76±3.79)s明显长于PBS组[(14.28±2.40)s],(P＜0.0).②缺血脑组织内的BrdU阳性细胞数PBS组为(12.17±3.49)个,实验组为(26.8±5.76)个,差异有显著性(P＜0.05).③脑血管共聚焦检测结果显示:EPC组较PBS组比较毛细血管内径显著性变小(分别为(2.8±0.2)μm与(3.4±0.24)μm,同源组织缺血边界地区的分支点数目显著增加[(分别为:(210.1±13.80)与(164.2±12.3)],EPC组可以显著增加微血管总面积(分别为:(84365±3897)与(74568±4626)μm2/0.002mm3),P＜0.05].④大鼠血浆VEGF检测[分别为(63.91±6.71)pg/ml;(21.81 ±4.25)pg/ml,P＜0.05]显示干预组明显增加(P＜0.05).结论 内皮祖细胞可以显著改善慢性脑缺血大鼠的学习记忆行为能力,其可能的机制是VEGF相关联的神经保护和血管再生,在治疗慢性脑缺血疾病中有着很大的应用前景.
目的 探討自體移植內皮祖細胞對慢性腦缺血大鼠行為學習功能的影響.方法 製作慢性腦缺血大鼠模型,靜脈自體移植內皮祖細胞,進行水迷宮檢測學習記憶功能,併從腦缺血區新生細胞、腦部血管、血管內皮生長因子(VEGF)等方麵探討可能的機製.結果 ①Morris水迷宮實驗中,訓練第2～5天的逃避潛伏期實驗組(EPC組)[分彆為(44.45 ±9.44)s,(38.32±1.51)s,(34.95±6.76)s,(24.46±5.47)s明顯短于對照組(PBS組)[對應為(52.79 ±6.47)s,(43.15±11.21)s,(50.29±11.41)s,(53.75±7.35)s](P＜0.01);第一象限遊泳時間EPC組(26.76±3.79)s明顯長于PBS組[(14.28±2.40)s],(P＜0.0).②缺血腦組織內的BrdU暘性細胞數PBS組為(12.17±3.49)箇,實驗組為(26.8±5.76)箇,差異有顯著性(P＜0.05).③腦血管共聚焦檢測結果顯示:EPC組較PBS組比較毛細血管內徑顯著性變小(分彆為(2.8±0.2)μm與(3.4±0.24)μm,同源組織缺血邊界地區的分支點數目顯著增加[(分彆為:(210.1±13.80)與(164.2±12.3)],EPC組可以顯著增加微血管總麵積(分彆為:(84365±3897)與(74568±4626)μm2/0.002mm3),P＜0.05].④大鼠血漿VEGF檢測[分彆為(63.91±6.71)pg/ml;(21.81 ±4.25)pg/ml,P＜0.05]顯示榦預組明顯增加(P＜0.05).結論 內皮祖細胞可以顯著改善慢性腦缺血大鼠的學習記憶行為能力,其可能的機製是VEGF相關聯的神經保護和血管再生,在治療慢性腦缺血疾病中有著很大的應用前景.
목적 탐토자체이식내피조세포대만성뇌결혈대서행위학습공능적영향.방법 제작만성뇌결혈대서모형,정맥자체이식내피조세포,진행수미궁검측학습기억공능,병종뇌결혈구신생세포、뇌부혈관、혈관내피생장인자(VEGF)등방면탐토가능적궤제.결과 ①Morris수미궁실험중,훈련제2～5천적도피잠복기실험조(EPC조)[분별위(44.45 ±9.44)s,(38.32±1.51)s,(34.95±6.76)s,(24.46±5.47)s명현단우대조조(PBS조)[대응위(52.79 ±6.47)s,(43.15±11.21)s,(50.29±11.41)s,(53.75±7.35)s](P＜0.01);제일상한유영시간EPC조(26.76±3.79)s명현장우PBS조[(14.28±2.40)s],(P＜0.0).②결혈뇌조직내적BrdU양성세포수PBS조위(12.17±3.49)개,실험조위(26.8±5.76)개,차이유현저성(P＜0.05).③뇌혈관공취초검측결과현시:EPC조교PBS조비교모세혈관내경현저성변소(분별위(2.8±0.2)μm여(3.4±0.24)μm,동원조직결혈변계지구적분지점수목현저증가[(분별위:(210.1±13.80)여(164.2±12.3)],EPC조가이현저증가미혈관총면적(분별위:(84365±3897)여(74568±4626)μm2/0.002mm3),P＜0.05].④대서혈장VEGF검측[분별위(63.91±6.71)pg/ml;(21.81 ±4.25)pg/ml,P＜0.05]현시간예조명현증가(P＜0.05).결론 내피조세포가이현저개선만성뇌결혈대서적학습기억행위능력,기가능적궤제시VEGF상관련적신경보호화혈관재생,재치료만성뇌결혈질병중유착흔대적응용전경.
Objective To study the effect of endothelial progenitor cells on the behavior of chronic cerebral ischemic rats. Methods Adult rats were treated using the protocol of chronic cerebral ischemic model. Then translated the endothelial progenitor cells in vein to them, and Morris water maze was carried out to test the learning and merrory ability of the rats. The cell proliferation, vascular distribution and the plasma VEGF levels were day of 2nd to 5th of experimental group ( EPC group ) were significantly shorter than the control group ( PBS group), which were(44.45 ±9.44)s,(38.32±1.51)s,(34.95 ±6.76)s,(24.46 ±5.47)s and (52.79±6.47 ) s, ( 43.15 ± 11.21 ) s, ( 50.29 ± 11.41 ) s, ( 53.75 ± 7.35 ) s, (P ＜ 0.01 ) respectively. The time of EPC group spend in the first quadrant were significantly longer than that of the PBS group, which were (26. 76 ±of the EPC group( 26.8 ± 5.76 ) was higher than that of the conrespondering areas in the control group( 12.17 ±ments of capillaries were (P＜0.05) shorter in the PBS groups( (3.4 ±0.24) μm) than in the EPC groups( (2.8± 0.2 )μm) significantly, EPC group could significantly (P ＜ 0.05 ) increased the number of branch points in the boundary regions of ischemia compared with the number in the PBS group (respectively (210. 1 ± 13.80 ) and (164.2 ± 12.3 )). Three-dimensional cerebral vessel surface area in the ipsilateral hemisphere significantly increased in the EPC group compared with the PBS group (respectively (84365 ± 3897 )μm2/0. 002mm3 and group in the plasma VEGF levels ( ( 63.91 ± 6.71 ) pg/ml; ( 21. 81 ± 4.25 ) pg/ml, respectively (P ＜ 0.05, P ＜0.01 ). Conclusion There are positive behavioral effects of endotbelial progenitor cells in chronic cerebral ischemic rats. The possible mechanisns mavbe involve the nerve protection and regeneration of the vascular associated with the VEGF. The endothelial progenitor cells maybe have a great prospect in the therapy of chronic cerebral ischemic disease.
