CAJ | 학술논문

目的通过分析RH血型检测方法,探讨影响RH检测结果的原因,确保实验室血型检测快捷、准确.方法对106 852例本血站献血员标本进行RH (D)血型抗原检测,通过盐水法、聚凝胺法、间接抗人球蛋白法对RH阴性血进行初筛、复查.结果受检者中检出RH (D)阴性316例,RH (D)阴性率为0.3％(316/106 852),其中检出RH Du亚型的3例.结论盐水法对RH阴性血型进行筛查,间接抗人球蛋白法和聚凝胺法结合确证其阴性的检测,能有效保证RH血型检测的准确性,对输血安全有重要意义.
목적 통과분석RH혈형검측방법,탐토영향RH검측결과적원인,학보실험실혈형검측쾌첩、준학.방법 대106 852례본혈참헌혈원표본진행RH (D)혈형항원검측,통과염수법、취응알법、간접항인구단백법대RH음성혈진행초사、복사.결과 수검자중검출RH (D)음성316례,RH (D)음성솔위0.3％(316/106 852),기중검출RH Du아형적3례.결론 염수법대RH음성혈형진행사사,간접항인구단백법화취응알법결합학증기음성적검측,능유효보증RH혈형검측적준학성,대수혈안전유중요의의.
Objective To explore the affecting factors for the detection of RH blood type and to ensure the labs to detect blood type rapidly and accurately by analyzing the methods of detecting RH blood type.Methods RH antigen was detected in the blood samples from 106 852 donators; the samples with negative RH ( D )were screened by normal saline and then re-tested by polybrene and indirect antihuman globulin.Results 316 of 106 852 ( 0.3％ ) donators were RH ( D ) negative,3 of whom had Du subtype.Conclusions Normal saline for screening RH ( D ) negative blood samples,and indirect antihuman globulin and polybrene for confirming RH ( D ) negative can effectively ensure the accuracy of detection of RH blood type and has important significance of blood transfusion safety.