国际输血及血液学杂志
國際輸血及血液學雜誌
국제수혈급혈액학잡지
INTERNATIONAL JOURNAL OF BLOOD TRANSFUSION AND HEMATOLOGY
2012年
5期
406-408
,共3页
鲍自谦%刘怡%王飞%陈云龙
鮑自謙%劉怡%王飛%陳雲龍
포자겸%류이%왕비%진운룡
全血%成份血%细菌内毒素%检测
全血%成份血%細菌內毒素%檢測
전혈%성빈혈%세균내독소%검측
whole blood%blood component%bacterial endotoxin%monitoring
目的 通过监测全血及成分血的内毒素含量动态变化,探讨内毒素监控的意义,为评估血液安全提供依据,保证安全输血.方法 选择2009年3月至4月本血液中心献血者中,符合国家《献血者健康检查要求》的自愿献血者血液样品300份为研究对象.按血液成分分为3组:全血组(n=50)、悬浮RBC成分血组(n=150)及PLT成分血组(n=100).采用动态浊度法内毒素定量检测技术分别于各组血液保存前期、中期及后期检测各个血液样品内毒素含量,并采用方差分析比较各个检测时间点血样内毒素变化情况.结果 全血保存期前中后期中,内毒素含量在部分时段差异有统计学意义(W=0.860,P<0.05);而PLT悬液、悬浮RBC在3个时间段的内毒素含量差异无统计学意义(P>0.05).结论 对全血及成分血的内毒素含量监测,结果显示血液保存期的内毒素含量稳定与其细菌培养阴性相吻合,表明现行的采供血流程的质量控制是有效的,可有效保障血液的安全.
目的 通過鑑測全血及成分血的內毒素含量動態變化,探討內毒素鑑控的意義,為評估血液安全提供依據,保證安全輸血.方法 選擇2009年3月至4月本血液中心獻血者中,符閤國傢《獻血者健康檢查要求》的自願獻血者血液樣品300份為研究對象.按血液成分分為3組:全血組(n=50)、懸浮RBC成分血組(n=150)及PLT成分血組(n=100).採用動態濁度法內毒素定量檢測技術分彆于各組血液保存前期、中期及後期檢測各箇血液樣品內毒素含量,併採用方差分析比較各箇檢測時間點血樣內毒素變化情況.結果 全血保存期前中後期中,內毒素含量在部分時段差異有統計學意義(W=0.860,P<0.05);而PLT懸液、懸浮RBC在3箇時間段的內毒素含量差異無統計學意義(P>0.05).結論 對全血及成分血的內毒素含量鑑測,結果顯示血液保存期的內毒素含量穩定與其細菌培養陰性相吻閤,錶明現行的採供血流程的質量控製是有效的,可有效保障血液的安全.
목적 통과감측전혈급성분혈적내독소함량동태변화,탐토내독소감공적의의,위평고혈액안전제공의거,보증안전수혈.방법 선택2009년3월지4월본혈액중심헌혈자중,부합국가《헌혈자건강검사요구》적자원헌혈자혈액양품300빈위연구대상.안혈액성분분위3조:전혈조(n=50)、현부RBC성분혈조(n=150)급PLT성분혈조(n=100).채용동태탁도법내독소정량검측기술분별우각조혈액보존전기、중기급후기검측각개혈액양품내독소함량,병채용방차분석비교각개검측시간점혈양내독소변화정황.결과 전혈보존기전중후기중,내독소함량재부분시단차이유통계학의의(W=0.860,P<0.05);이PLT현액、현부RBC재3개시간단적내독소함량차이무통계학의의(P>0.05).결론 대전혈급성분혈적내독소함량감측,결과현시혈액보존기적내독소함량은정여기세균배양음성상문합,표명현행적채공혈류정적질량공제시유효적,가유효보장혈액적안전.
Objective To explore the importance of dynamic monitoring on the bacterial endotoxin and assess the blood safety to guarantee the safety of blood transfusion,through dynamically monitoring the bacterial endotoxin concentration in whole blood and blood component. Methods From March to April 2009,300 donors' samples,which had met the requirements of donors' health check,were included in this study.According to the blood component,all these samples were performed into three groups:whole blood group (n=50),red blood cells (RBC) suspension group (n=150),platelet (PLT) components group (n=100).Dynamic turbidity in blood which were respectively stored at early stage,metaphase and later stage,were used as the detection quantitative method of bacterial endotoxin.And the results were further analyzed by variance of repeated measurement data. Results There was no difference of the bacterial endotoxin concentration during the preservation in PLT suspension and RBC suspension(P>0.05).Meanwhile,there was significant difference of the bacterial endotoxin concentration between preservation periods of the whole blood(P<0.05).Conclusions Through dynamically monitoring the bacterial endotoxin concentration in whole blood and blood component,the stability of bacterial endotoxin concentration during the preservation period coincides with a negative result of the bacterial culture.It could be shown that the quality control of the blood collection and supply procedure is effective to guarantee the safety of blood.