CAJ | 학술논문

最近的一些研究结果显示,视皮层内抑制性递质系统作用减弱可能是导致老年性视觉功能衰退的重要因素.是否皮层内兴奋性递质系统亦伴随衰老而发牛改变并影响皮层内神经兴奋与抑制的平衡尚不清楚.为此,利用Nissl染色和免疫组织化学染色方法以及Image-Pro Express图像分析软件对青、老年猫初级视皮层(17区)内各层神经元密度、兴奋性递质谷氨酸免疫反应阳性(Glu-immunoreactive,Glu-IR)神经元密度以及抑制性递质γ-氨基丁酸免疫反应阳性(γ-aminobutyric acid.immunoreactive,GABA-IR)神经元密度进行了统汁分析.结果显示,青、老年猫初级视皮层各层神经元密度均没有明显的年龄性差异(P>0.05);与青年猫相比,老年猫初级视皮层Glu-IR、GABA-IR神经元密度均显著减少(P<0.01),而Glu.IR/GABA.IR神经元密度比率去却显著增大(P<0.01).结果提示,老年猫初级视皮层内兴奋性递质系统作用相对增强,而抑制性递质系统的作用相对减弱,导致皮层内兴奋-抑制平衡关系失调,这可能是引起老年个体视觉功能衰退的重要原因之一.
최근적일사연구결과현시,시피층내억제성체질계통작용감약가능시도치노년성시각공능쇠퇴적중요인소.시부피층내흥강성체질계통역반수쇠로이발우개변병영향피층내신경흥강여억제적평형상불청초.위차,이용Nissl염색화면역조직화학염색방법이급Image-Pro Express도상분석연건대청、노년묘초급시피층(17구)내각층신경원밀도、흥강성체질곡안산면역반응양성(Glu-immunoreactive,Glu-IR)신경원밀도이급억제성체질γ-안기정산면역반응양성(γ-aminobutyric acid.immunoreactive,GABA-IR)신경원밀도진행료통즙분석.결과현시,청、노년묘초급시피층각층신경원밀도균몰유명현적년령성차이(P>0.05);여청년묘상비,노년묘초급시피층Glu-IR、GABA-IR신경원밀도균현저감소(P<0.01),이Glu.IR/GABA.IR신경원밀도비솔거각현저증대(P<0.01).결과제시,노년묘초급시피층내흥강성체질계통작용상대증강,이억제성체질계통적작용상대감약,도치피층내흥강-억제평형관계실조,저가능시인기노년개체시각공능쇠퇴적중요원인지일.
Recent studies show that a reduced effect of inhibitory transmitter system in the visual cortex may underlie aged visual function degradation. Whether excitatory transmitter system changes with age and hence affects intracortical excitation-inhibition balance is not clear. To explore this issue, we used Nissl staining and immunohistochemical methods as well as Image-Pro Express software to examine the density of Nissl-stained neurons, Glutamic acid-immunoreactive (Glu-IR) neurons and γ-Aminobutyric acid-immunoreactive (GABA-IR) neurons in the primary visual cortex of young adult and aged cats. The results showed that there was no significant difference in the density of Nissl-stained neurons between young and old cats (P>0.05). However, the density of Giu-IR neurons and GABA-IR neurons in the primary visual cortex of aged cats was significantly lower than that of young ones (P<0.01). The ratio between Glu-IR neurons and GABA-IR neurons was significantly increased in old cats compared to that in young adult ones (P<0.01). These results indicated that the effect of excitatory transmitter system in the old visual cortex was increased relative to the inhibitory transmitter system, which might cause an imbalance between cortical excitation and inhibition and might be an important factor mediating the visual function decline during aging.