CAJ | 학술논문

采用RT-PCR方法扩增黄瓜绿斑驳花叶病毒的外壳蛋白基因与3,非编码区,并将其构建到马铃薯X病毒(PVX)载体中.重组质粒经线性化及体外转录后接种烟草,获得含有病毒外壳蛋白基因的感病植株.感病组织可用于分子检测的质控,也可作为毒源来繁殖阳性参照物质.基于PVX载体制备的参照物质可降低检疫性病毒的生物安全风险,尤其对稳定性强、可造成严重经济损失的高风险病毒的检疫更具实用价值.
채용RT-PCR방법확증황과록반박화협병독적외각단백기인여3,비편마구,병장기구건도마령서X병독(PVX)재체중.중조질립경선성화급체외전록후접충연초,획득함유병독외각단백기인적감병식주.감병조직가용우분자검측적질공,야가작위독원래번식양성삼조물질.기우PVX재체제비적삼조물질가강저검역성병독적생물안전풍험,우기대은정성강、가조성엄중경제손실적고풍험병독적검역경구실용개치.
The CP gene and 3'noncoding region of Cucumber green mottle mosaic virus (CGMMV) were construc-ted into the PVX based virus vector p45P46. In vitro RNA transcripts from recombinant plasmid pPVXCCMMV -CP were mechanically inoculated onto Nicotiana benthamiana. The diseased plants could be utilized as vires source and the reference material for molecular detection. Preparation of the reference material of CGMMV based on PVX vector can reduce biosafety risk effectively. The method in this study provides a feasible protocol for pro-ducing reference materials of plant viruses, especially for those which were highly stable and could cause great e-conomic losses.