CAJ | 학술논문

目的:研究血管紧张素转化酶抑制剂(ACE-I)联合奈达铂(NDP)在体外对鼻咽癌细胞生长的影响.方法:利用MTT比色法、流式细胞仪、RT-PCR、免疫印迹及酶联免疫等方法测定不同组(对照组、NDP组、ACE-I组、NDP+ACE-I组)对人鼻咽癌细胞CNE2生长、细胞周期及对血管内皮生长因子(VEGF)表达的影响.结果:单独NDP或ACE-I不能抑制CNE2细胞生长,联合应用能明显抑制CNE2细胞生长和VEGF的表达.结论:ACE-I和NDP联合抑制CNE2细胞增殖是通过抑制VEGF的表达实现的.
목적:연구혈관긴장소전화매억제제(ACE-I)연합내체박(NDP)재체외대비인암세포생장적영향.방법:이용MTT비색법、류식세포의、RT-PCR、면역인적급매련면역등방법측정불동조(대조조、NDP조、ACE-I조、NDP+ACE-I조)대인비인암세포CNE2생장、세포주기급대혈관내피생장인자(VEGF)표체적영향.결과:단독NDP혹ACE-I불능억제CNE2세포생장,연합응용능명현억제CNE2세포생장화VEGF적표체.결론:ACE-I화NDP연합억제CNE2세포증식시통과억제VEGF적표체실현적.
Objective To investigate the effect of angiotensin--converting enzyme inhibitor combined with nedaplatin on the growth of nasoph,taryngeal carcinoma CNE2 cells in vitro.Methods The effects of ACEI combined with nedaplatin on cell proliferation,cell cycle and VEGF expression of CNE2 were detected with MTr assay,flow cytometry,RT-PCR,Western blotting and ELISA,respectively.Results Neither NDP nor ACE-I had significant inhibitory effect on the CNE2 growth,while the combined application had significant inhibitory effect on the CNE2 growth VEGF expression.Conclusion The proliferation of CNE2 CoLdd be inhibited with the combination of ACE-I and NDP by inhibiting VEGF expression.