CAJ | 학술논문

以猪喉管为原料提取硫酸软骨素,使用超声波辅助手段进行碱处理,浸出液再用木瓜蛋白酶进一步去除蛋白质.结果表明:最佳工艺条件为超声波处理时间139min、超声功率491W、木瓜蛋白酶酶解pH6.8、酶加入量1.3:1000(mg/mL)、酶解温度45℃、酶解时间1h,该工艺制备得到硫酸软骨素为白色粉末得率达23.15%,澄清度0.038,蛋白质含量2.48%,氨基己糖含量30.52%,硫酸软骨素纯度86.06%,水分6.32%,pH6.7,各项指标完全符合国家相关标准要求.
이저후관위원료제취류산연골소,사용초성파보조수단진행감처리,침출액재용목과단백매진일보거제단백질.결과표명:최가공예조건위초성파처리시간139min、초성공솔491W、목과단백매매해pH6.8、매가입량1.3:1000(mg/mL)、매해온도45℃、매해시간1h,해공예제비득도류산연골소위백색분말득솔체23.15%,징청도0.038,단백질함량2.48%,안기기당함량30.52%,류산연골소순도86.06%,수분6.32%,pH6.7,각항지표완전부합국가상관표준요구.
Porcine pipes were used as the raw material to extract chondroitin sulfate based on ultrasonic-assisted alkaline treaunent followed by papain hydrolysis for removing residual protein.The optimal ulWasonic-assisted alkaline treatment and papain hydrolysis processes were explored using response surface methodology and orthogonal array design,respectively.Results indicated that the optimal extraction process of chondroitin sulfate was based on ultrasonic-assistecl treatment for 139 rain at 491W power followed by papnin hydrolysis for 1 h at pH 6.8 and 45 ℃ with an enzyme dose of 1.3:1000(mg/mL).After drying in an oven set at 55-60 ℃,chondroitin sulfate obtained using the optimal process was characterized by white color,23.15% extraction yield,0.038 clarity,2.48% protein content,86.06% purity,6.32% moisture content and pH 6.7.All quality indicators completely reach the requirements of relevant national standards for chondroitin sulfate.