中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2011年
28期
17-20
,共4页
呼吸道合胞病毒%基质金属蛋白酶9%转化生长因子β1%氢化可的松
呼吸道閤胞病毒%基質金屬蛋白酶9%轉化生長因子β1%氫化可的鬆
호흡도합포병독%기질금속단백매9%전화생장인자β1%경화가적송
Respiratory syncytial virus%Matrix metalloproteinase 9%Transforming growth factorbeta 1%Hydrocortisone
目的 通过测定急性呼吸道合胞病毒( RSV)感染时内源性血浆皮质醇浓度和气道重塑相关因子,揭示RSV感染时内源性血浆皮质醇和气道重塑相关因子之间的关系.方法 选择42例急性RSV感染患儿,其中重症21例(重症组),轻症21例(轻症组),另选择21例同期健康婴儿作为对照组.ELISA方法检测血清基质金属蛋白酶-9(MMP-9)、金属蛋白酶组织抑制物-1(TIMP-1)及转化生长因子-β1(TGF-β1)水平,放射免疫分析法检测血浆皮质醇水平.结果 重症组血浆皮质醇水平为(236.25±119.87)μg/L,与轻症组的(130.62±73.04)μg/L和对照组的(56.35±34.52)μg/L比较差异有统计学意义(P＜0.05),轻症组与对照组比较差异有统计学意义(P＜0.05).重症组血清TIMP-1和TGF-β1水平高于轻症组(P＜0.05),轻症组高于对照组(P＜0.05)；重症组MMP-9/TIMP-1低于对照组(P＜0.05)；重症组血清MMP-9水平高于轻症组(P＜0.05).重症组淋巴细胞、嗜酸粒细胞、CD4+、CD8+均较轻症组和对照组降低(P＜0.05)；轻症组嗜酸粒细胞、CD8+均较对照组降低(P＜0.05).结论 RSV感染可导致患儿气道重塑相关因子失衡,存在气道重塑的高危因素,可能与血浆皮质醇水平增高有关,从而导致难治性喘息性疾病的存在.
目的 通過測定急性呼吸道閤胞病毒( RSV)感染時內源性血漿皮質醇濃度和氣道重塑相關因子,揭示RSV感染時內源性血漿皮質醇和氣道重塑相關因子之間的關繫.方法 選擇42例急性RSV感染患兒,其中重癥21例(重癥組),輕癥21例(輕癥組),另選擇21例同期健康嬰兒作為對照組.ELISA方法檢測血清基質金屬蛋白酶-9(MMP-9)、金屬蛋白酶組織抑製物-1(TIMP-1)及轉化生長因子-β1(TGF-β1)水平,放射免疫分析法檢測血漿皮質醇水平.結果 重癥組血漿皮質醇水平為(236.25±119.87)μg/L,與輕癥組的(130.62±73.04)μg/L和對照組的(56.35±34.52)μg/L比較差異有統計學意義(P＜0.05),輕癥組與對照組比較差異有統計學意義(P＜0.05).重癥組血清TIMP-1和TGF-β1水平高于輕癥組(P＜0.05),輕癥組高于對照組(P＜0.05)；重癥組MMP-9/TIMP-1低于對照組(P＜0.05)；重癥組血清MMP-9水平高于輕癥組(P＜0.05).重癥組淋巴細胞、嗜痠粒細胞、CD4+、CD8+均較輕癥組和對照組降低(P＜0.05)；輕癥組嗜痠粒細胞、CD8+均較對照組降低(P＜0.05).結論 RSV感染可導緻患兒氣道重塑相關因子失衡,存在氣道重塑的高危因素,可能與血漿皮質醇水平增高有關,從而導緻難治性喘息性疾病的存在.
목적 통과측정급성호흡도합포병독( RSV)감염시내원성혈장피질순농도화기도중소상관인자,게시RSV감염시내원성혈장피질순화기도중소상관인자지간적관계.방법 선택42례급성RSV감염환인,기중중증21례(중증조),경증21례(경증조),령선택21례동기건강영인작위대조조.ELISA방법검측혈청기질금속단백매-9(MMP-9)、금속단백매조직억제물-1(TIMP-1)급전화생장인자-β1(TGF-β1)수평,방사면역분석법검측혈장피질순수평.결과 중증조혈장피질순수평위(236.25±119.87)μg/L,여경증조적(130.62±73.04)μg/L화대조조적(56.35±34.52)μg/L비교차이유통계학의의(P＜0.05),경증조여대조조비교차이유통계학의의(P＜0.05).중증조혈청TIMP-1화TGF-β1수평고우경증조(P＜0.05),경증조고우대조조(P＜0.05)；중증조MMP-9/TIMP-1저우대조조(P＜0.05)；중증조혈청MMP-9수평고우경증조(P＜0.05).중증조림파세포、기산립세포、CD4+、CD8+균교경증조화대조조강저(P＜0.05)；경증조기산립세포、CD8+균교대조조강저(P＜0.05).결론 RSV감염가도치환인기도중소상관인자실형,존재기도중소적고위인소,가능여혈장피질순수평증고유관,종이도치난치성천식성질병적존재.
Objective To explore the relationship between the endogenous plasma cortisol and the airway remodeling associated cytokines which was infected respiratory syncytial virus (RSV) through measuring the levels of them.Methods Forty-two infants diagnosed as acute RSV infection were divided into two groups,severe group(21 cases) and mild group(21 cases),as well as 21 healthy infants for control group.The levels of matrix metalloproteinase-9 (MMP-9),tissue inhibitor of metalloproteinase-1 (TIMP-1) and transforming growth factor- β1 (TGF- β 1) were determined by ELISA,the levels of plasma cortisol were determined by radio immunoaasay.Results The levels of plasma cortisol in severe group [(236.25 ± 119.87)μg/L] were significantly higher than those in mild group [(130.62 ±73.04)μg/L] and control group [(56.35 ±34.52)μg/L],there were significant differences (P ＜0.05),and the mild group was significandy higher than the control group(P＜ 0.05).The levels of TIMP-1 and TGF- β 1 in severe group were significantly higher than those in mild group(P＜ 0.05),and the mild group was significantly higher than the control group (P ＜ 0.05 ); the ratio of MMP-9 and TIMP-1 in severe group was significantly lower than that in control group (P ＜0.05);the levels of MMP-9 in severe group were significantly higher than those in mild group (P ＜0.05).The levels of lymphocytes,eosinophilic cells and CD4+,CD8+ in severe group were significantly lower than those in mild group (P＜0.05),and significantly lowerthan those in control group (P ＜ 0.05 ).The levels of eosinophilic cells and CD8+ in mild group were significantly lower than those in control group(P ＜ 0.05 ).Conclusion The infection of RSV results in the imbalance of the airway remodeling associated cytokines,which is a danger factor,and the imbalance may be related to the increased level of the plasma cortisol.
