中华围产医学杂志
中華圍產醫學雜誌
중화위산의학잡지
CHINESE JOURNAL OF PERINATAL MEDICINE
2011年
6期
354-358
,共5页
张玉真%王娟%乔立兴%蒋犁
張玉真%王娟%喬立興%蔣犛
장옥진%왕연%교립흥%장리
视网膜新生血管化%缺氧诱导因子-1α%酸中毒%动物,新生%大鼠
視網膜新生血管化%缺氧誘導因子-1α%痠中毒%動物,新生%大鼠
시망막신생혈관화%결양유도인자-1α%산중독%동물,신생%대서
Retinal neovascularization%Hypoxia-inducible factor 1,alpha subunit%Acidosis%Animals,newborn%Rats
目的 探讨缺氧诱导因子-lα(hypoxia-inducible factor-lα,HIF-lα)在代谢性酸中毒诱导视网膜新生血管中的表达和意义.方法 将120只新生SD大鼠随机分为酸中毒模型组和正常对照组,各60只.酸中毒模型组出生后第2天开始按535 mg/(kg·d)的剂量管饲氯化铵溶液 (质量浓度为50 mg/ml),2次/d,连续6 d,停药进入恢复期.2组大鼠分别于出生后第3、5、8、10、13、20天处死.二磷酸腺苷酶组织化学染色法观察新生大鼠视网膜血管的形态改变;HE染色观察视网膜血管增生情况,判断建模成功与否;免疫组织化学方法检测视网膜HIF-1α蛋白的表达变化.结果 二磷酸腺苷酶组织化学染色法显示酸中毒模型组大鼠10日龄可见视网膜大量新生血管异常形成,周边可见大片无灌注区.HE染色可见酸中毒模型组新生大鼠10日龄视网膜突破内界膜血管内皮细胞核显著多于同龄对照组,分别为(28.78±7.53)个与(1.22±1.48)个,差异有统计学意义(t=11.169,P<0.01).免疫组织化学结果显示,酸中毒模型组新生大鼠8、10、13日龄时视网膜HIF-1α的表达强度分别为108.87±15.21、183.68±26.58和129.42±9.85,明显高于同龄对照组,分别为74.98±4.50、76.38±3.38和74.78±1.86,差异均有统计学意义(t=4.625、9.023和9.672,P均<0.05).结论 HIF-1α在酸中毒诱导视网膜新生血管的发生、发展过程中可能发挥重要作用.
目的 探討缺氧誘導因子-lα(hypoxia-inducible factor-lα,HIF-lα)在代謝性痠中毒誘導視網膜新生血管中的錶達和意義.方法 將120隻新生SD大鼠隨機分為痠中毒模型組和正常對照組,各60隻.痠中毒模型組齣生後第2天開始按535 mg/(kg·d)的劑量管飼氯化銨溶液 (質量濃度為50 mg/ml),2次/d,連續6 d,停藥進入恢複期.2組大鼠分彆于齣生後第3、5、8、10、13、20天處死.二燐痠腺苷酶組織化學染色法觀察新生大鼠視網膜血管的形態改變;HE染色觀察視網膜血管增生情況,判斷建模成功與否;免疫組織化學方法檢測視網膜HIF-1α蛋白的錶達變化.結果 二燐痠腺苷酶組織化學染色法顯示痠中毒模型組大鼠10日齡可見視網膜大量新生血管異常形成,週邊可見大片無灌註區.HE染色可見痠中毒模型組新生大鼠10日齡視網膜突破內界膜血管內皮細胞覈顯著多于同齡對照組,分彆為(28.78±7.53)箇與(1.22±1.48)箇,差異有統計學意義(t=11.169,P<0.01).免疫組織化學結果顯示,痠中毒模型組新生大鼠8、10、13日齡時視網膜HIF-1α的錶達彊度分彆為108.87±15.21、183.68±26.58和129.42±9.85,明顯高于同齡對照組,分彆為74.98±4.50、76.38±3.38和74.78±1.86,差異均有統計學意義(t=4.625、9.023和9.672,P均<0.05).結論 HIF-1α在痠中毒誘導視網膜新生血管的髮生、髮展過程中可能髮揮重要作用.
목적 탐토결양유도인자-lα(hypoxia-inducible factor-lα,HIF-lα)재대사성산중독유도시망막신생혈관중적표체화의의.방법 장120지신생SD대서수궤분위산중독모형조화정상대조조,각60지.산중독모형조출생후제2천개시안535 mg/(kg·d)적제량관사록화안용액 (질량농도위50 mg/ml),2차/d,련속6 d,정약진입회복기.2조대서분별우출생후제3、5、8、10、13、20천처사.이린산선감매조직화학염색법관찰신생대서시망막혈관적형태개변;HE염색관찰시망막혈관증생정황,판단건모성공여부;면역조직화학방법검측시망막HIF-1α단백적표체변화.결과 이린산선감매조직화학염색법현시산중독모형조대서10일령가견시망막대량신생혈관이상형성,주변가견대편무관주구.HE염색가견산중독모형조신생대서10일령시망막돌파내계막혈관내피세포핵현저다우동령대조조,분별위(28.78±7.53)개여(1.22±1.48)개,차이유통계학의의(t=11.169,P<0.01).면역조직화학결과현시,산중독모형조신생대서8、10、13일령시시망막HIF-1α적표체강도분별위108.87±15.21、183.68±26.58화129.42±9.85,명현고우동령대조조,분별위74.98±4.50、76.38±3.38화74.78±1.86,차이균유통계학의의(t=4.625、9.023화9.672,P균<0.05).결론 HIF-1α재산중독유도시망막신생혈관적발생、발전과정중가능발휘중요작용.
Objective To investigate the expression and significance of hypoxia-inducible factor-1α (HIF-1α) in the retinal neovascularization by metabolic acidosis in newborn rats. Methods One hundred and twenty newborn SD rats were randomly divided into acidosis (experiment) and normoxia (control) groups. A total of 60 newborn rats in experiment group underwent tubal feeding day for 6 days and followed by a period of recovery. The rats in the two groups were sacrificed at the 3rd, 5th, 8th, 10th, 13th and 20th day after birth, respectively. The morphologic changes of retinal vessels were estimated by observing the vascular pattern in adenosine diphosphatase stained retina flat mounts. The newborn vessels were quantified by HE staining. Immunohistochemical method was used to detect HIF-1α expression. Results In experiment group, numerous neovascularization and un-perfused area at the periphery of vessels occurred on the 10th day. The result of HE staining showed that in experiment group of 10-day old,the number of neovascular nuclei extending into the vireo was 28.78±7.53, and that of the control group was 1.22±1.48 (t=11.169,P<0.01). The results of immunohistochemistry revealed that the expression of HIF-1α protein were stronger in the experiment group than in the control group on the 8th, 10th and 13th day, and there were significant differences between the two groups (108.87±15.21, 183.68±26.58 and 129.42±9.85 vs 74.98±4.50, 76.38± 3.38 and 74.78±1.86, t=4.625, 9.023 and 9.672,P<0.05). Conclusions HIF-lα might play an important role in retinal neovascularization.
